1. Oxygen saturation and perfusion changes during dermatological methylaminolaevulinate photodynamic therapy.
- Author
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Tyrrell J, Thorn C, Shore A, Campbell S, and Curnow A
- Subjects
- Aged, 80 and over, Aminolevulinic Acid therapeutic use, Female, Humans, Male, Microcirculation, Skin blood supply, Skin Diseases blood, Skin Temperature physiology, Aminolevulinic Acid analogs & derivatives, Oxygen blood, Photochemotherapy methods, Photosensitizing Agents therapeutic use, Skin Diseases drug therapy
- Abstract
Background: Methylaminolaevulinate (MAL)-photodynamic therapy (PDT) is a successful topical treatment for a number of (pre)cancerous dermatological conditions. In combination, light of the appropriate wavelength, the photosensitizer protoporphyrin IX (PpIX) and tissue oxygen result in the production of singlet oxygen and reactive oxygen species inducing cell death., Objectives: This study investigates real-time changes in localized tissue blood oxygen saturation and perfusion in conjunction with PpIX fluorescence monitoring for the first time during dermatological MAL-PDT., Methods: Oxygen saturation, perfusion and PpIX fluorescence were monitored noninvasively utilizing optical reflectance spectroscopy, laser Doppler perfusion imaging and a fluorescence imaging system, respectively. Patients attending for standard dermatological MAL-PDT were recruited to this ethically approved study and monitored prior to, during and after light irradiation., Results: Significant reductions in mean blood oxygen saturation (P < 0·005) and PpIX fluorescence (P < 0·001) were observed within the first minute of irradiation (4·75 J cm(-2) ) while, in contrast, perfusion was observed to increase significantly (P < 0·01) during treatment. The changes in oxygen saturation and PpIX fluorescence were positively correlated during the initial phase of treatment (r(2) = 0·766)., Conclusions: Rapid reductions in the localized blood oxygen saturation have been observed for the first time to occur clinically within the initial minutes of light irradiation and positively correlate with the concurrent PpIX photobleaching. Furthermore, perfusion increases, suggesting that the microvasculature compensates for the PDT-induced oxygen depletion., (© 2011 The Authors. BJD © 2011 British Association of Dermatologists 2011.)
- Published
- 2011
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