1. Bio-catalytic nanocompartments for in situ production of glucose-6-phosphate.
- Author
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Lomora M, Gunkel-Grabole G, Mantri S, and Palivan CG
- Subjects
- Glucose-6-Phosphate chemistry, Nanoparticles chemistry, Particle Size, Phosphoglucomutase chemistry, Biocatalysis, Glucose-6-Phosphate biosynthesis, Nanoparticles metabolism, Phosphoglucomutase metabolism
- Abstract
Cells are sophisticated biocatalytic systems driving a complex network of biochemical reactions. A bioinspired strategy to create advanced functional systems is to design confined spaces for complex enzymatic reactions by using a combination of synthetic polymer assemblies and natural cell components. Here, we developed bio-catalytic nanocompartments that contain phosphoglucomutase protected by a biomimetic polymer membrane, which was permeabilized for reactants through insertion of an engineered α-hemolysin pore protein. These bio-catalytic nanocompartments serve for production of glucose-6-phosphate, and thus possess great potential for applications in an incomplete glycolysis, pentose phosphate pathway, or in plant biological reactions.
- Published
- 2017
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