Search

The Langmuir monolayers of L-α-phosphatidylcholine from egg yolk were studied by Wilhelmy method. The surface pressure versus molecular area isotherm of lipid on pure water and different subphase (with a presence of divalent ions: Sr2+, Cd2+, Ba2+, Pb2+) was obtained. The limiting area of the isotherms depends on the presence of subphase ions. The addition of divalent ions to the subphase stabilized the monolayers and increased the limiting areas of the monolayer. During the compression in monolayer complexes of 1:1 and 2:1 stoichiometry between L-α-phosphatidylcholine from egg yolk and divalent ions are formed. We used the equilibrium theory to describe the behavior of monolayer components at the air/water interface. An equilibrium theory to describe the behavior of monolayer components at the air/water interface was developed in order to obtain the stability constants and area occupied by one molecule of LMe2+ or L2Me2+ complexes, and complex formation energy (Gibbs free energy) values. These mathematically derived and experimentally confirmed values are of great importance for the interpretation of phenomena occurring in lipid monolayers and bilayers.

Influence of sodium salts of selected chaotropic anions from the Hofmeister series (NaCl, NaBr, NaNO3, NaI) on the surface charge density of phosphatidylcholine membranes was studied. Small unilamellar lipid vesicles were used as a model system in the investigations. The theoretical and experimental approach to the interactions between inorganic anions and phosphatidylcholine membranes is presented. Experimental membrane surface charge densities data were determined as a function of pH of the aqueous electrolytes using microelectrophoresis method. The quantitative description of the interactions between zwitterionic phosphatidylcholine membrane and monovalent anions is presented. The equilibria constants of the binding of solution ions onto phospholipid surface were calculated. Knowledge of these parameters was essential to determine the theoretical membrane surface charge density values. The theoretical data were compared to the experimental ones in order to verify the mathematical model. Both approaches indicate that the anion-phosphatidylcholine membrane interaction increases with the size of the anion. The adsorption of chaotropic anions to membranes was found to follow the Hofmeister series I&minus, &gt, NO3&minus, Br&minus, Cl&minus

Quercetin is a naturally-occurring flavonoid claimed to exert many beneficial health effects. In this report, the influence of quercetin on the surface charge of phosphatidylcholine liposomes and human glioblastoma LN-229 and LN-18 cells was studied using microelectrophoretic mobility measurements. The effect of quercetin on the electrical resistance and capacitance of bilayer lipid membranes was analyzed via electrochemical impedance spectroscopy. The results showed that after flavonoid treatment, the cell lines demonstrated changes in surface charge only in alkaline pH solutions, whereas there were no significant alterations in quercetin-treated vs. control cells in acidic pH solutions. The same tendency was found for liposomal membranes proving that quercetin insertion into membranes is strongly pH-dependent. Capacitance and resistance measurements conducted in acidic electrolyte solutions demonstrated an increase in both electrical parameters, indicating an increased amount of quercetin inserted into the bilayers. Moreover, the cytotoxic effect of quercetin confirms that the flavonoid enters the cells and perturbs the proliferation of LN-229 and LN-18 glioblastoma cell lines. As such, our results indicate that the specific localization of quercetin, membrane-bound or cell-entering, might be crucial for its pharmacological activity. However, further studies are necessary prior to applying these physicochemical measurements as standard methods of evaluating drug activity.

Bilayer lipid membranes composed of phosphatidylcholine and isoleucine or phosphatidylcholine and tyrosine were investigated using electrochemical impedance spectroscopy. Interaction between membrane components causes significant deviations from the additivity rule which can be explained by formation of the domain structures. The surface area of domains was calculated based on derived equations. We suggest that the stoichiometry of phosphatidylcholine–isoleucine domain is equal 3:1. In the case of tyrosine-modified phosphatidylcholine membranes, domain with stoichiometry 7:1 should be taken into consideration.

We have monitored the effect of ergosterol on electrical capacitance and electrical resistance of the phosphatidylcholine bilayer membranes using chronopotentiometry method. The chronopotentiometric characteristic of the bilayers depends on constant-current flow through the membranes. For low current values, no electroporation takes place and the membrane voltage rises exponentially to a constant value described by the Ohm's law. Based on these kinds of chronopotentiometric curves, a method of the membrane capacitance and the membrane resistance calculations is presented.

The interfacial tension of lipid membranes composed of phosphatidylcholine (lecithin, PC)-valine (Val), phosphatidylcholine-isoleucine (Ile), phosphatidylcholine-tyrosine (Tyr), and phosphatidylcholine-phenylalanine (Phe) has been studied. The membrane components formed 1:1 complexes. The interfacial tension measurements were used to determine the membrane surface concentration A (3)(-1), the membrane interfacial tension γ(3), and the stability constant K.

Interfacial tension has been determined for phosphatidylcholine-cholesterol, phosphatidylcholine-phosphatidylethanolamine, and phosphatidylethanolamine-cholesterol membranes. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), and cholesterol (Ch) were to be investigated, because of their presence in biological membranes. Interfacial tension values of pure components are 0.81×10−3 N/m, 1.67×10−3 N/m, and 2.36×10−3 N/m, respectively. The 1∶1 complexes were formed during formation of the PC-Ch, PC-PE, and PE-Ch lipid membranes. The following parameters describing the complexes were determined: A 3 −1 , the surface concentrations of the lipid membranes formed from these complexes; γ3, the interfacial tensions of such membranes and K, the stability constants of these complexes.

The effect of ergosterol on the electrochemical features of the phosphatidylcholine bilayer membrane was investigated by impedance spectroscopy. The experimental impedance values obtained in the presence of different amounts of ergosterol showed evidence of domain structures within the bilayer containing

The effect of the presence of valinomycin in lecithin membrane on its interfacial tension has been studied. The experiments have been carried out at various forming solution compositions and at various potassium ion concentrations in electrolyte solution. Potassium chloride was used as the electrolyte. A complex was formed between the valinomycin molecule and K + ion. The following parameters describing the complex were determined: K , the stability constant of the valinomycin–K + complex and B , partition coefficient. These values are equal to 3.52 × 10 5 m 3 mol −1 and 6.0, respectively.

The effect of alpha-tocopherol on the electrochemical features of the phosphatidylcholine membrane was investigated by impedance spectroscopy. Phosphatidylcholine and alpha-tocopherol were chosen for the study because they are present in biological membranes and they fulfill essential functions in living organisms. The experimental impedance values obtained in the presence of different amounts of alpha-tocopherol showed evidence of domain structures within the bilayer containing less than 0.048 molar fraction of alpha-tocopherol. Based on derived mathematical equations, the surface area of phospholipid/alpha-tocopherol domain was calculated; it amounts to 832 A(2). This value is consistent, taking into consideration ordering and condensation effects of alpha-tocopherol, with the acknowledged, well documented, stoichiometry of such a domain of 10:1. The result of the investigation is the proposal of a new method for the determination of the surface area and description of the stoichiometry of domains formed in any two-component system.

The effect of the presence of gramicidin D in a lecithin membrane on its interfacial tension has been studied. The studies have been carried out at various forming solution compositions and at various potassium ion concentrations in the electrolyte solution. Potassium chloride was used as the electrolyte. The complex was formed between the gramicidin molecule and K + ion. The following parameters describing the complex were determined: the surface area occupied by GK + complex ( A GK + ), the interfacial tension of the GK + membrane complex ( γ GK + ), and the stability constant of the gramicidin–K + complex ( K ). These values are 156 A 2 , 1.89 mN m −1 and 0.033 m 3 mol −1 , respectively.

This paper reports measurements on the pH dependence of the electrical capacitance of lipid membranes formed by 1:1 phosphatidylcholine-phosphatidylserine mixtures. A theoretical model was developed to describe this dependence, in which the contributions of functional groups (as the active centers of adsorption of the hydrogen and hydroxide ions) to the overall membrane capacitance were assumed to be additive. The proposed model was verified experimentally using electrochemical impedance spectroscopy. The theoretical predictions agreed with the experimental results over the measured pH range. A minimum corresponding to the isoelectric point appeared in both the theoretical equation and the experimental data.

Monolayers of phosphatidylcholine, tyrosine, and phenylalanine and binary mixtures phosphatidylcholine–tyrosine or phosphatidylcholine–phenylalanine were investigated at the air/water interface. Phosphatidylcholine (lecithin, PC), tyrosine (Tyr), and phenylalanine (Phe) were used in the experiment. The surface tension values of pure and mixed monolayers were used to calculate π–A isotherms. The surface tension measurements were carried out at 22°C using an improved Teflon trough and a Nima 9000 tensiometer. The Teflon trough was filled with a subphase of triple-distilled water. Known amounts of lipid dissolved in 1-chloropropane were placed at the surface using a syringe. The interactions between lecithin and amino acid result in significant deviations from the additivity rule. An equilibrium theory to describe the behavior of monolayer components at the air/water interface was developed in order to obtain the stability constants of PC–Tyr as well as PC–Phe complexes. We considered the equilibrium between the individual components and the complex and established that lecithin and amino acid formed highly stable 1:1 complex.

The basic electrical parameters of bilayer lipid membranes are capacitance and resistance. This article describes the application of chronopotentiometry to the research of lipid bilayers. Membranes were made from egg yolk phosphatidylcholine. The chronopotentiometric characteristic of the membranes depends on the current value. For low current values, no electroporation takes place and the voltage rises exponentially to a constant value. Based on these kinds of chronopotentiometric curves, a method of the membrane capacitance and the membrane resistance calculations are presented.

Electrochemical impedance spectroscopy was used for the study of two-component lipid membranes. Phosphatidylcholine and ceramide were to be investigated, since they play an important biochemical role in cell membranes. The research on biolipid interaction was focused on quantitative description of processes that take part in a bilayer. Assumed models of interaction between amphiphilic molecules and the equilibria that take place there were described by mathematical equations for the studied system. The possibility of complex formation for two-component system forming bilayers was assumed that could explain the deviation from additivity rule. Equilibria were described by mathematical equations that were further verified experimentally. The determined values of parameters (stability constant, molecular area of complex, capacitance and conductance of the lipid membranes formed from molecules and complexes) were used for calculation of model curves. The comparison of model curves and experimental points verified the assumed model.

Interfacial tension has been determined for phosphatidylcholine–stearic acid and phosphatidylcholine–stearylamine membranes. Phosphatidylcholine, stearic acid and stearylamine were used in the experimental. The interfacial tension values of the pure components are 1.62 × 10− 3 N/m, − 1.54 × 10− 2 N/m and 4.40 × 10− 3 N/m (hypothetical values), respectively. The 1:1 complexes were formed during formation of phosphatidylcholine–stearic acid and phosphatidylcholine–stearylamine membranes. The following parameters describing the complexes were determined: the surface concentrations of the lipid membranes formed from these complexes, A3− 1, the interfacial tensions of such membranes, γ3 and the stability constants of these complexes, K.

The effect of the ion carrier valinomycin on the electrochemical features of the phosphatidylcholine membrane was investigated by electrochemical impedance spectroscopy. Phosphatidylcholine and valinomycin were chosen for the study because they fulfil essential functions in lively organisms. The experimental impedance values obtained in the presence of different amounts of carrier, studied with several potassium ion concentrations, were used for the research ability of valinomycin to form a 1:1 potassium ion complex on the lipid bilayer/electrolyte solution interface. Based on derived mathematical equations, the heterogeneous equilibrium constant (K (h)), association rate constant of the complex (k (R)) and dissociation rate constant of the complex (k (D)) were calculated. The result of the investigation is the proposal of a new method for the determination of the parameters used to describe the chemical reaction at the interface between a carrier molecule from the membrane and a monovalent ion from the aqueous phase.

Electrochemical impedance spectroscopy (EIS) was used to the study of gramicidin D (GD) dimerization and to transport of monovalent cations across lipid bilayers by the dimers. Phosphatidylcholine (PC) membranes were studied, unmodified and modified with very low GD concentrations in the presence of various potassium ion concentrations. A new method was proposed to determine the parameters used to describe the gramicidin dimer: gramicidin surface concentration ( c GG ), area occupied by individual channel ( A G ) and gramicidin dimerization equilibrium constant ( K GG ). It was shown that electrochemical impedance spectroscopy measurements of lipid bilayer membranes yielded the K GG and A G values of the same order of magnitude as other measurement techniques.

Capacity and electric resistance of lipid membranes composed of lecithin and cholesterol were determined. The components were chosen for the study because they were present in biological membranes. Capacitance of the lecithin and cholesterol membranes amounts to 0.38 and 0.61 microF/cm(2), and resistance to 1.44(10(4)and 2.12(10(6)Omega cm(2), respectively. A 1:1 complex appears as a result of lecithin-cholesterol membrane formation. Parameters of the membrane formed of the lecithin-cholesterol complex were determined: surface concentration (Gamma(3)), capacitance (C(3)), and conductance (R;(3)(-1), as well as the stability constant (K) of the complex. The mean values of those magnitudes are as follows: 4.265(10(-6)mol/m(2), 0.54 microF/cm(2), 1.381(10(-6)Omega(-1)cm(-2)and 3.748(10(7), respectively.

Bilayer lipid membranes composed of phosphatidylcholine and decanoic acid or phosphatidylcholine and decylamine were investigated using electrochemical impedance spectroscopy. Interaction between membrane components causes significant deviations from the additivity rule. Area, capacitance, and stability constant values for the complexes were calculated based on the model assuming 1:1 stoichiometry, and the model was validated by comparison of these values to experimental results. We established that phosphatidylcholine and decylamine form highly stable 1:1 complexes. In the case of decanoic acid-modified phosphatidylcholine membranes, complexes with stoichiometries other than 1:1 should be taken into consideration.

This paper describes the application of chronopotentiometry to lipid bilayer research. The experiments were performed on bilayer lipid membranes composed of phosphatidylcholine and cholesterol and formed using the painting technique. Chronopotentiometric (U = f(t)) measurements were used to determine the membrane capacitance, resistance, and breakdown voltage as well as pore conductance and diameter.