7 results on '"Criado, Maria"'
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2. Phloem transport of assimilates in relation to flowering time and senescence in barley grown with different availabilities of nitrogen and phosphorus.
- Author
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Criado, Maria V., Roberts, Irma N., Veliz, Cintia G., Echeverria, Mariela, Gutierrez Boem, Flavio H., and Caputo, Carla
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BARLEY yields , *PHLOEM , *FLOWERING time , *CELLULAR aging , *BARLEY , *EFFECT of nitrogen on plants , *EFFECT of phosphorus on plants , *PHYSIOLOGY - Abstract
Understanding the way in which N and P availability affects the transport of sugar and amino acids is crucial to improve grain quality and yield. Thus, in the present study, two greenhouse and field experiments were conducted with barley plants grown with different N and P availabilities to assess the dynamics of the phloem transport of assimilates in relation to the beginning of flowering and senescence. The phloem transport of assimilates decreased before the beginning of protein degradation in all treatments, but the onset of flowering and senescence varied according to the N and P availability, as evidenced by the concentrations of proteins, amino acids, and sugar and the gene expression of senescence-related proteases and all glutamine synthetase isoforms. In N-deficient plants, the phloem transport decreased before flowering, but only when P was not limiting; in N- and P-sufficient plants it decreased at flowering; and in P-deficient plants it decreased after flowering. Therefore, only N-deficient but P-sufficient plants have a post-anthesis period with high export rate of assimilates. This alteration of phenology in relation to phloem leads to important consequences in assimilate utilization, as shown by the higher yield and N content of the former compared to P-deficient plants. [ABSTRACT FROM AUTHOR]
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- 2018
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3. Phloem sugars and amino acids as potential regulators of hordein expression in field grown malting barley (Hordeum vulgare L.).
- Author
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Veliz, Cintia G., Criado, Maria Victoria, Roberts, Irma N., Echeverria, Mariela, Prystupa, Pablo, Prieto, Paula, Gutierrez Boem, Flavio H., and Caputo, Carla
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PHLOEM , *SUGARS , *AMINO acids , *HORDEIN , *BARLEY , *CEREAL products - Abstract
The commercial quality of malting barley is dependent on the content and composition of grain proteins which are subjected to nitrogen (N) and sulfur (S) control. In nutrient deficient soils, grain protein content is mainly a consequence of the remobilization efficiency. In order to evaluate the effect of N and S soil availability on phloem amino acid and sugar export rates and B- and C-hordein gene expression during grain filling, a factorial combination of N and S fertilization assay was carried out under field conditions. Besides, several carbon (C) and N metabolites were analyzed in leaves and spikes. We observed that, even under soil N and S availabilities that do not limit yield, N and S fertilization induced significant changes in N and C metabolism. N phloem remobilization was promoted by S fertilization independently of N fertilization and C remobilization was also promoted by S fertilization but only in N fertilized plants. The B- and C-hordein gene expression correlated positively with sugar and amino acid exudation rate, respectively. Our results suggest an important role of the export rate of sugars and amino acids in the regulation of grain prolamine expression. [ABSTRACT FROM AUTHOR]
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- 2014
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4. Sulphur deficiency inhibits nitrogen assimilation and recycling in barley plants
- Author
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Cintia G. Veliz, Carla Caputo, Maria Victoria Criado, and Irma N. Roberts
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HORDEUM VULGARE ,0106 biological sciences ,0301 basic medicine ,Otras Ciencias Biológicas ,Nitrogen assimilation ,Plant Science ,Horticulture ,Biology ,01 natural sciences ,Ciencias Biológicas ,03 medical and health sciences ,Glutamine synthetase ,Botany ,Gene expression ,Gene ,chemistry.chemical_classification ,AMINO ACID TRANSPORTERS ,PHLOEM AMINO ACID EXUDATION ,fungi ,food and beverages ,Assimilation (biology) ,Amino acid ,030104 developmental biology ,Biochemistry ,chemistry ,Phloem ,Hordeum vulgare ,GLUTAMINE SYNTHETASE ,CIENCIAS NATURALES Y EXACTAS ,010606 plant biology & botany - Abstract
Sulphur (S) is incorporated into diverse primary and secondary metabolites that play important roles in proper growth and development of plants. In cereals, a fraction of the nitrogen (N) accumulated in developing grains is guaranteed by amino acid remobilization from vegetative tissues, a contribution that becomes critical when soil nutrients are deficient. Glutamine synthetase (GS) and amino acid transporters (AAT) are key components involved in N assimilation and recycling. The aim of the present study was to evaluate the effect of S availability on the expressions of HvGS and several selected HvAAT genes in barley plants and on the phloem exudation rate of amino acids. To this end, two independent experiments were designed to impose low S availability conditions to barley plants. Low S availability caused a decrease in the phloem exudation rate of amino acids as well as in the gene expression of all the HvGS genes and five of the six HvAAT genes analyzed. The strong correlation found between the phloem amino acid exudation rate and HvGS1-1, HvGS1-2, HvAAP7, and HvProT1 gene expression may indicate the participation of these genes in the regulation of amino acid remobilization through the phloem. Fil: Veliz, Cintia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Roberts, Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Criado, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Caputo Suarez, Carla Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
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- 2017
- Full Text
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5. Phloem transport of amino acids is differentially altered by phosphorus deficiency according to the nitrogen availability in young barley plants
- Author
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Maria Victoria Criado, Carla Caputo, Irma N. Roberts, and Cintia G. Veliz
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0106 biological sciences ,0301 basic medicine ,Physiology ,Nitrogen assimilation ,BARLEY ,Dehydrogenase ,PHLOEM TRANSPORT ,Plant Science ,Biology ,01 natural sciences ,Ciencias Biológicas ,03 medical and health sciences ,AMINO ACIDS ,Glutamine synthetase ,Botany ,Phloem transport ,SINK-SOURCE TRANSITION ,Phosphorus deficiency ,Amino acid export ,Ciencias de las Plantas, Botánica ,chemistry.chemical_classification ,fungi ,food and beverages ,Amino acid ,030104 developmental biology ,Biochemistry ,chemistry ,Phloem ,LOW PHOSPHORUS AND NITROGEN SUPPLY ,Agronomy and Crop Science ,CIENCIAS NATURALES Y EXACTAS ,010606 plant biology & botany - Abstract
The phloem transport of amino acids is a key step in the efficient use of nitrogen (N). Despite the importance of this issue, little information is known about the regulation of phloem transport of amino acids in plants with low phosphorus (P) supply and even less in relation to N availability. To this end, we studied not only the assimilate partitioning in young barley plants grown with low N or/and P supply, but also we analyzed the implications of the different isoforms of glutamine synthetase, cytokinin oxidase/dehydrogenase 2 and several senescence-related proteases. Our results demonstrated that low P supply causes an accumulation of different nitrogenous compounds in expanded leaves depending on N availability and an inhibition of the phloem exudation rate of amino acids only in high-N plants, indicating an interaction between N and P in the establishment of N-partitioning. The accumulation of nitrogenous compounds in leaves of low-P plants without the accompaniment in amino acid export to the phloem was not related to an increase in nitrate assimilation pathway neither with the modulation of glutamine synthetase 1_1 expression, as it had been observed for N availability. But, these results could be explained as a consequence of a delay in the transition from sink to source of leaves, thus keeping the older leaves as sink organs, as indicated by the increase in cytokinin oxidase/dehydrogenase 2 expression and the repression of several senescence-related proteases in low-P plants with good availability of N. Fil: Criado, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Veliz, Cintia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Roberts, Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina Fil: Caputo Suarez, Carla Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina
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- 2017
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6. Cytokinin-induced changes of nitrogen remobilization and chloroplast ultrastructure in wheat (Triticum aestivum)
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Atilio J. Barneix, Irma N. Roberts, Carla Caputo, Maria Victoria Criado, and María A. Castro
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Chloroplasts ,Cytokinins ,Nitrogen ,Physiology ,NITROGEN STARVATION ,Plant Science ,Protein degradation ,TRITICUM AESTIVUM ,Nitrate reductase ,Polymerase Chain Reaction ,chemistry.chemical_compound ,BENZYLAMINOPURINE ,Botany ,Sugar ,Triticum ,DNA Primers ,Base Sequence ,biology ,Agricultura ,fungi ,RuBisCO ,CHLOROPLAST ,food and beverages ,NITROGEN REMOBILIZATION ,Chloroplast ,Microscopy, Electron ,chemistry ,CIENCIAS AGRÍCOLAS ,Chlorophyll ,Cytokinin ,biology.protein ,Phloem ,Agricultura, Silvicultura y Pesca ,Agronomy and Crop Science - Abstract
Nitrogen (N) remobilization in wheat (Triticum aestivum) plants is crucial because it determines the grain protein concentration and the baking quality of flour. In order to evaluate the influence of cytokinins on N remobilization during N starvation, we analyzed various N remobilization parameters in wheat plants that were watered with 6-benzylaminopurine (BAP) either with or without KNO3. Besides, the effects of BAP on protein synthesis were evaluated, and the size and ultrastructure of chloroplasts of BAP-treated plants were studied. BAP supply inhibited N remobilization of plants independently of N supply as shown by the increase in protein, Rubisco, chlorophyll, sugar and starch concentrations in the older leaves, the decrease in amino acid and sugar export to the phloem, and the decrease in protein, Rubisco and chlorophyll concentrations in the younger leaves. Besides, BAP supply increased nitrate reductase activity and decreased nitrate concentration, thus suggesting an increased assimilatory capacity. The increase in protein concentration could be explained mainly by a significant decrease in protein degradation and, to a lesser extent, by an increase in protein synthesis. Finally, an increase both in the size of the chloroplast and in the plastoglobuli and starch contents in BAP-supplied plants was observed. We propose that cytokinins retain the sink activity of the older leaves by inhibiting amino acid and sugar export to the phloem and stimulating assimilate accumulation in the chloroplasts of the older leaves. Besides, BAP may increase protein concentration of the older leaves both by decreasing protein degradation and maintaining protein synthesis even under stress conditions. © 2009 Elsevier GmbH. All rights reserved. Fil: Criado, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina Fil: Caputo Suarez, Carla Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina Fil: Roberts, Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina Fil: Castro, Maria A.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina Fil: Barneix, Atilio José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
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- 2009
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7. Regulation of glutamine synthetase 1 and amino acids transport in the phloem of young wheat plants
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M. Victoria Criado, Carla Caputo, Atilio J. Barneix, M. Alejandra Gelso, and Irma N. Roberts
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Physiology ,Otras Ciencias Biológicas ,PHLOEM TRANSPORT ,Plant Science ,Biology ,Phloem ,TRITICUM AESTIVUM ,Gene Expression Regulation, Enzymologic ,Ciencias Biológicas ,chemistry.chemical_compound ,AMINO ACIDS ,N DEFICIENCY ,Gene Expression Regulation, Plant ,Glutamate-Ammonia Ligase ,Glutamine synthetase ,Methionine Sulfoximine ,Benzyl Compounds ,Genetics ,Phloem transport ,Amino Acids ,Phylogeny ,Triticum ,Plant Proteins ,chemistry.chemical_classification ,Nitrates ,Reverse Transcriptase Polymerase Chain Reaction ,fungi ,food and beverages ,Plant physiology ,Biological Transport ,Kinetin ,BAP ,Enzyme assay ,Amino acid ,Glutamine ,Isoenzymes ,chemistry ,Biochemistry ,Purines ,Cytokinin ,biology.protein ,Electrophoresis, Polyacrylamide Gel ,GLUTAMINE SYNTHETASE ,CIENCIAS NATURALES Y EXACTAS - Abstract
The possible regulation of amino acid remobilization via the phloem in wheat (Triticum aestivum L.) by the primary enzyme in nitrogen (N) assimilation and re-assimilation, glutamine synthetase (GS, E.C. 6.3.1.2) was studied using two conditions known to alter N phloem transport, N deficiency and cytokinins. The plants were grown for 15 days in controlled conditions with optimum N supply and then N was depleted from and/or 6-benzylaminopurine was added to the nutrient solution. Both treatments generated an induction of GS1, monitored at the level of gene expression, protein accumulation and enzyme activity, and a decrease in the exudation of amino acids to the phloem, obtained with EDTA technique, which correlated negatively. GS inhibition by metionine sulfoximide (MSX) produced an increase of amino acids exudation and the inhibitor successfully reversed the effect of N deficiency and cytokinin addition over phloem exudation. Our results point to an important physiological role for GS1 in the modulation of amino acids export levels in wheat plants. © 2009 Elsevier Masson SAS. All rights reserved. Fil: Caputo Suarez, Carla Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina Fil: Criado, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina Fil: Roberts, Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina Fil: Gelso, María Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina Fil: Barneix, Atilio José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Invetigaciones Bioquímicas y Fisiologicas; Argentina
- Published
- 2008
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