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Background: Visceral leishmaniasis (VL) known as Kala-Azar is a serious systemic disease caused by Leishmania donovani parasites (Trypanosomatidae: Kinetoplastida). The disease is prevalent in the Indian Sub-continent, East Africa and Brazil. In Africa, the worst affected regions are in Sudan, with an estimated 15,000-20,000 cases annually and Ethiopia with 5,000-7,000 cases a year. The main vector of VL in Sudan and Northern Ethiopia is Phlebotomus orientalis, a sand fly frequently found in association with Acacia spp and Balanite spp woodlands., Methods: To optimize sampling of sand flies for epidemiological studies in remote areas we tested different means of attraction. Miniature suction traps employing 2AA batteries (3 V) were deployed in the up-draft orientation and baited with chemical light-sticks (Red, Yellow and Green), or bakers' yeast in sugar solution (emitting CO2 and perhaps other attractants). These traps were compared with standard CDC incandescent light traps employing 6 V batteries. Trials were conducted during two consecutive years at different localities around Sheraro, a town in West Tigray, Northern Ethiopia., Results: The sand fly species composition was similar but not identical in the different locations tested with different Sergentomyia spp. predominating. Phlebotomus spp. comprised less than 1% of the catches during the first year trials (November - December 2011) but increased markedly during the second year trials performed later in the dry season at the height of the sand fly season in February 2012. Although there did not appear to be a species bias towards different light wave-lengths, fermenting yeast in sugar solution attracted relatively more Phlebotomus spp. and Sergentomyia schwetzi., Conclusions: Although the standard 6 V CDC incandescent light traps captured more sand flies, light-weight (~350 g) 3 V suction traps baited with chemical light-sticks were shown to be effective means of monitoring sand flies. Such traps operated without light and baited with yeast in sugar solution caught relatively more Phlebotomus spp.

Female phlebotomine sand flies (Diptera: Psychodidae) transmit leishmaniasis as they engorge on vertebrate blood required for egg production. Phlebotomus (Phlebotomus) papatasi (Scopoli, 1786), the vector of Leishmania major (Yakimoff & Schokhor, 1914), the causative agent of cutaneous leishmaniasis (CL) were not attracted to large horizontal sticky traps (LHSTs) unless these were baited with CO2 derived from dry ice or from fermenting sugar/yeast mixture (SYM). Attraction of P. papatasi males by CO2 may indicate their tendency to mate on or near the blood-host. Male P. (Larroussius) orientalis (Parrot, 1936), the vector of visceral leishmaniasis (VL) in Ethiopia, were collected on LHSTs in large numbers. Although the number of females remained low, augmentation with SYM, increased the number of females by 800% while the number of males increased by only about 40%. Apparently, male P. orientalis utilize the horizontal surfaces for forming mating swarms. P. (Paraphlebotomus) sergenti (Parrot, 1917), is the vector of CL caused by Leishmania tropica. Although approximately twice as many P. sergenti males were caught on LHSTs as females, it appears that LHSTs were attractive to both sexes. Use of SYM baits is potentially useful for monitoring phlebotomine sand flies in places where dry ice is unobtainable or prohibitively expensive. LHSTs can provide an inexpensive alternative to CDC traps for monitoring some species of sand flies. Unfortunately, the numbers of female sand flies, crucial for estimating transmission of Leishmania, is usually low on LHSTs., (Copyright © 2013 Elsevier B.V. All rights reserved.)

Phlebotomine sand flies transmit Leishmania, phlebo-viruses and Bartonella to humans. A prominent gap in our knowledge of sand fly biology remains the ecology of their immature stages. Sand flies, unlike mosquitoes do not breed in water and only small numbers of larvae have been recovered from diverse habitats that provide stable temperatures, high humidity and decaying organic matter. We describe studies designed to identify and characterize sand fly breeding habitats in a Judean Desert focus of cutaneous leishmaniasis. To detect breeding habitats we constructed emergence traps comprising sand fly-proof netting covering defined areas or cave openings. Large size horizontal sticky traps within the confined spaces were used to trap the sand flies. Newly eclosed male sand flies were identified based on their un-rotated genitalia. Cumulative results show that Phlebotomus sergenti the vector of Leishmania tropica rests and breeds inside caves that are also home to rock hyraxes (the reservoir hosts of L. tropica) and several rodent species. Emerging sand flies were also trapped outside covered caves, probably arriving from other caves or from smaller, concealed cracks in the rocky ledges close by. Man-made support walls constructed with large boulders were also identified as breeding habitats for Ph. sergenti albeit less important than caves. Soil samples obtained from caves and burrows were rich in organic matter and salt content. In this study we developed and put into practice a generalized experimental scheme for identifying sand fly breeding habitats and for assessing the quantities of flies that emerge from them. An improved understanding of sand fly larval ecology should facilitate the implementation of effective control strategies of sand fly vectors of Leishmania.

The control of the sand fly vectors of leishmaniasis is problematic because their larvae develop in largely unknown terrestrial habitats making them impervious to available control measures. Furthermore, the behavior patterns of adults of different sand fly species are highly diverse, requiring tailor-made control solutions based upon a profound knowledge of their biology. In this short review, we describe possible lines of research that hold promise for improving our munitions in the battle against the diseases they transmit. The suggested approaches are not necessarily presented in order of importance, but rather in a logical sequence starting in the larval breeding areas where the sand flies originate and culminating with the human environments. Some examples are offered to illustrate the potential efficacy., (© 2011 The Society for Vector Ecology.)

In 2006/7, 18 cases of cutaneous leishmaniasis (CL) were reported for the first time from Sde Eliyahu (pop. 650), a village in the Beit She'an valley of Israel. Between 2007–2011, a further 88 CL cases were diagnosed bringing the total to 106 (16.3% of the population of Sde Eliyahu). The majority of cases resided in the south-western part of the village along the perimeter fence. The causative parasite was identified as Leishmania major Yakimoff & Schokhor, 1914 (Kinetoplastida: Trypanosomatidae). Phlebotomus papatasi (Scopoli), 1786 (Diptera: Psychodidae) was found to be the most abundant phlebotomine species comprising 97% of the sand flies trapped inside the village, and an average of 7.9% of the females were positive for Leishmania ITS1 DNA. Parasite isolates from CL cases and a sand fly were characterized using several methods and shown to be L. major. During a comprehensive survey of rodents 164 Levant voles Microtus guentheri Danford & Alston, 1880 (Rodentia: Cricetidae) were captured in alfalfa fields bordering the village. Of these 27 (16.5%) tested positive for Leishmania ITS1 DNA and shown to be L. major by reverse line blotting. A very high percentage (58.3% - 21/36) of Tristram's jirds Meriones tristrami Thomas, 1892 (Rodentia: Muridae), found further away from the village also tested positive for ITS1 by PCR. Isolates of L. major were successfully cultured from the ear of a wild jird found positive by ITS1 PCR. Although none of the wild PCR-positive voles exhibited external pathology, laboratory-reared voles that were infected by intradermal L. major inoculation, developed patent lesions and sand flies became infected by feeding on the ears of these laboratory-infected voles. This is the first report implicating M. guentheri and M. tristrami as reservoirs of Leishmania. The widespread co-distribution of M. guentheri and P. papatasi, suggests a significant threat from the spread of CL caused by L. major in the Middle East, central Asia and southern Europe. Author Summary: This study dealt with the ecological and epidemiological characterization of a newly emerged dermal disease caused by Leishmania parasites transmitted by the bite of infected sand flies. In the first five years of its emergence, the human infection rate in Kibbutz Sde Eliyahu, Israel reached 16%. The majority of cases resided along the periphery of the Kibbutz. To identify the vector species, we performed systematic trapping of sand flies in different habitats inside and outside the inhabited areas. Sand flies were identified in the laboratory and shown to comprise mainly Phlebotomus papatasi. Since the causative agent of human cases was shown to be L. major, we examined sand flies for Leishmania infections by PCR and found a high infection rate amongst sand flies trapped near houses in late summer (11.8%). To confirm the Leishmania species, we isolated live parasites from the gut of a wild-caught sand fly female. The cultured isolate was identified as L. major by several methods. The Levant vole, abundant in the surrounding fields and Tristram's jird found further away from the Kibbutz were tested for infection rate and their capacity for hosting the parasite and were both incriminated as new possible hosts. [ABSTRACT FROM AUTHOR]