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4 results on '"retinol isotope dilution"'

1. Filipino Children with High Usual Vitamin A Intakes and Exposure to Multiple Sources of Vitamin A Have Elevated Total Body Stores of Vitamin A But Do Not Show Clear Evidence of Vitamin A Toxicity

Author

Engle-Stone, Reina, Miller, Jody C, Reario, Maria Fatima Dolly, Arnold, Charles D, Stormer, Ame, Lafuente, Eleanore, Oxley, Anthony, Capanzana, Mario V, Cabanilla, Carl Vincent D, Ford, Jennifer Lynn, Clark, Adam, Velavan, Thirumalaisamy P, Brown, Kenneth H, Lietz, Georg, and Haskell, Marjorie J

Subjects
Prevention, Nutrition, Liver Disease, Digestive Diseases, Clinical Trials and Supportive Activities, Clinical Research, 3.3 Nutrition and chemoprevention, Prevention of disease and conditions, and promotion of well-being, Oral and gastrointestinal, vitamin A, dietary intake, retinol isotope dilution, total body stores, toxicity, supplements, fortified foods, children, Philippines
Abstract

BackgroundYoung children exposed to high-dose vitamin A supplements (VAS) and vitamin A (VA)-fortified foods may be at risk of high VA intake and high VA total body stores (TBS).ObjectivesTBS and estimated liver VA concentration were compared among children with adequate or high VA intake and different timing of exposure to VAS, and associations between estimated liver VA concentrations and biomarkers of VA toxicity were examined.MethodsChildren 12-18 mo of age (n = 123) were selected for 3 groups: 1) retinol intake >600 µg/d and VAS within the past mo, 2) retinol intake >600 µg/d and VAS in the past 3-6 mo, and 3) VA intake 200-500 µg retinol activity equivalents (RAE)/d and VAS in the past 3-6 mo. Dietary intake data were collected to measure VA intakes from complementary foods, breast milk, and low-dose, over-the-counter supplements. TBS were assessed by retinol isotope dilution, and VA toxicity biomarkers were measured. Main outcomes were compared by group.ResultsMean (95% CI) VA intakes excluding VAS were 1184 (942, 1426), 980 (772, 1187), and 627 (530, 724) µg RAE/d, in groups 1-3, respectively; mean VA intake was higher in groups 1 and 2 compared with group 3 (P 1 µmol/g liver). There was no evidence of chronic VA toxicity, suggesting that the liver VA cutoff value should be re-evaluated. This trial was registered at www.clinicaltrials.gov as NCT03030339.

Published
2022

2. Determination of Vitamin A Total Body Stores in Children from Dried Serum Spots: Application in a Low- and Middle-Income Country Community Setting

Author

Oxley, Anthony, Engle-Stone, Reina, Miller, Jody C, Reario, MF Dolly, Stormer, Ame, Capanzana, Mario V, Cabanilla, Carl VD, Haskell, Marjorie J, and Lietz, Georg

Subjects
Clinical Research, Clinical Trials and Supportive Activities, Pediatric, Prevention, Nutrition, Digestive Diseases, Chromatography, Liquid, Developing Countries, Diterpenes, Female, Humans, Indicator Dilution Techniques, Infant, Isotopes, Liver, Male, Nutrition Assessment, Nutritional Status, Philippines, Plasma, Refrigeration, Reproducibility of Results, Retinyl Esters, Serum, Tandem Mass Spectrometry, Temperature, Vitamin A, Vitamin A Deficiency, vitamin A status, total body stores, retinol isotope dilution, dried serum spots, dried blood spots, stable isotopes, liquid chromatography tandem mass spectrometry, children, Animal Production, Food Sciences, Nutrition and Dietetics, Nutrition & Dietetics
Abstract

BackgroundThe retinol isotope dilution (RID) method has been used to evaluate vitamin A (VA) status in healthy adults and children in low- and middle-income countries (LMIC) and to assess the efficacy of various VA interventions.ObjectiveThe study was designed to examine whether dried serum spots (DSS) can be applied to RID when conducting VA total body store (TBS) assessments in community settings.MethodsFour days after an oral dose of 0.4 mg [13C10]retinyl acetate was administered to Filipino children (12-18 mo), a single blood draw was divided to isolate both serum and plasma. Serum (40 μL) was spotted and dried on Whatman 903 cards and shipped at ambient temperature whereas liquid plasma (LP) was frozen at -80°C and shipped on dry ice. The VA tracer to tracee ratio from DSS and LP was quantified by LC-MS/MS. Comparisons between DSS and LP paired samples (n = 72) were made for [13C10]retinol specific activity (SAp) by Pearson's correlation and for VA TBS by Bland-Altman analysis.ResultsThe sum of 3 coextracted DSS were required to consistently detect [13C10]retinol above the LC-MS/MS limit of quantitation (LOQ). [13C10]retinol SAp from DSS was highly correlated with SAp from LP (r = 0.945; P

Published
2021

3. Filipino Children with High Usual Vitamin A Intakes and Exposure to Multiple Sources of Vitamin A Have Elevated Total Body Stores of Vitamin A But Do Not Show Clear Evidence of Vitamin A Toxicity

Author

Reina Engle-Stone, Jody C Miller, Maria Fatima Dolly Reario, Charles D Arnold, Ame Stormer, Eleanore Lafuente, Anthony Oxley, Mario V Capanzana, Carl Vincent D Cabanilla, Jennifer Lynn Ford, Adam Clark, Thirumalaisamy P Velavan, Kenneth H Brown, Georg Lietz, and Marjorie J Haskell

Subjects
and promotion of well-being, Nutrition and Dietetics, Philippines, Prevention, Liver Disease, Clinical Trials and Supportive Activities, toxicity, Medicine (miscellaneous), total body stores, Prevention of disease and conditions, vitamin A, Oral and gastrointestinal, supplements, children, Clinical Research, dietary intake, Digestive Diseases, 3.3 Nutrition and chemoprevention, retinol isotope dilution, fortified foods, Nutrition, Food Science
Abstract

BackgroundYoung children exposed to high-dose vitamin A supplements (VAS) and vitamin A (VA)-fortified foods may be at risk of high VA intake and high VA total body stores (TBS).ObjectivesTBS and estimated liver VA concentration were compared among children with adequate or high VA intake and different timing of exposure to VAS, and associations between estimated liver VA concentrations and biomarkers of VA toxicity were examined.MethodsChildren 12-18mo of age (n=123) were selected for 3 groups: 1) retinol intake >600µg/d and VAS within the past mo, 2) retinol intake >600µg/dand VAS in the past 3-6mo, and 3) VA intake 200-500µg retinol activity equivalents (RAE)/d and VAS in the past 3-6mo. Dietary intake data were collected to measure VA intakes from complementary foods, breast milk, and low-dose, over-the-counter supplements. TBS were assessed by retinol isotope dilution, and VA toxicity biomarkers were measured. Main outcomes were compared by group.ResultsMean (95% CI) VA intakes excluding VAS were 1184 (942, 1426), 980 (772, 1187), and 627 (530, 724) µg RAE/d, in groups 1-3, respectively; mean VA intake was higher in groups 1 and 2 compared with group 3 (P1µmol/g liver). There was no evidence of chronic VA toxicity, suggesting that the liver VA cutoff value should be re-evaluated. This trial was registered at www.clinicaltrials.gov as NCT03030339.

Published
2022

4. Determination of Vitamin A Total Body Stores in Children from Dried Serum Spots: Application in a Low- and Middle-Income Country Community Setting

Author

Mario Capanzana, M F Dolly Reario, Carl Cabanilla, Ame Stormer, Georg Lietz, Marjorie J Haskell, Anthony Oxley, Reina Engle-Stone, and Jody C Miller

Subjects
0301 basic medicine, Serum, Male, liquid chromatography tandem mass spectrometry, Retinyl Esters, Philippines, Medicine (miscellaneous), Indicator Dilution Techniques, Retinyl acetate, chemistry.chemical_compound, Plasma, 0302 clinical medicine, Isotopes, Refrigeration, Tandem Mass Spectrometry, Medicine, 030212 general & internal medicine, Vitamin A, Methodology and Mathematical Modeling, dried serum spots, Pediatric, Chromatography, Liquid, Nutrition and Dietetics, Spots, Vitamin A Deficiency, Retinol, Temperature, Total body, total body stores, Liver, dried blood spots, Community setting, Female, Diterpenes, retinol isotope dilution, Vitamin, Clinical Trials and Supportive Activities, Nutritional Status, stable isotopes, 03 medical and health sciences, AcademicSubjects/MED00060, Animal science, Food Sciences, Paired samples, children, Animal Production, Clinical Research, Humans, Developing Countries, Nutrition, 030109 nutrition & dietetics, Nutrition & Dietetics, business.industry, Prevention, Reproducibility of Results, Infant, vitamin A status, Nutrition Assessment, chemistry, AcademicSubjects/SCI00960, Low and middle income, business, Digestive Diseases, Chromatography, Liquid
Abstract

Author(s): Oxley, Anthony; Engle-Stone, Reina; Miller, Jody C; Reario, MF Dolly; Stormer, Ame; Capanzana, Mario V; Cabanilla, Carl VD; Haskell, Marjorie J; Lietz, Georg | Abstract: BackgroundThe retinol isotope dilution (RID) method has been used to evaluate vitamin A (VA) status in healthy adults and children in low- and middle-income countries (LMIC) and to assess the efficacy of various VA interventions.ObjectiveThe study was designed to examine whether dried serum spots (DSS) can be applied to RID when conducting VA total body store (TBS) assessments in community settings.MethodsFour days after an oral dose of 0.4nmg [13C10]retinyl acetate was administered to Filipino children (12-18nmo), a single blood draw was divided to isolate both serum and plasma. Serum (40 μL) was spotted and dried on Whatman 903 cards and shipped at ambient temperature whereas liquid plasma (LP) was frozen at -80°C and shipped on dry ice. The VA tracer to tracee ratio from DSS and LP was quantified by LC-MS/MS. Comparisons between DSS and LP paired samples (nn=n72) were made for [13C10]retinol specific activity (SAp) by Pearson's correlation and for VA TBS by Bland-Altman analysis.ResultsThe sum of 3 coextracted DSS were required to consistently detect [13C10]retinol above the LC-MS/MS limit of quantitation (LOQ). [13C10]retinol SAp from DSS was highly correlated with SAp from LP (rn=n0.945; P ln0.01). A comparison of methods for TBS determination using Bland-Altman analysis indicated agreement with an intraindividual difference of 24.7nμmol (4.6%). Mean total liver reserve (TLR) values from DSS and LP were 1.7nμmol/g (± 0.6 SD) and 1.6nμmol/g (± 0.6 SD), respectively.ConclusionsVA TBS can be determined from DSS thereby reducing the logistics and cost of maintaining a cold chain by shipping samples at ambient temperature and, thus, making the RID technique more feasible in LMIC community settings. This trial was registered at https://clinicaltrials.gov as NCT03030339.

Published
2021

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