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As part of the multifaceted strategies developed to shape the common environmental policy, considerable attention is now being paid to assessing the degree of environmental degradation in soil under xenobiotic pressure. Bisphenol A (BPA) has only been marginally investigated in this ecosystem context. Therefore, research was carried out to determine the biochemical properties of soils contaminated with BPA at two levels of contamination: 500 mg and 1000 mg BPA kg -1 d.m. of soil. Reliable biochemical indicators of soil changes, whose activity was determined in the pot experiment conducted, were used: dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase, arylsulfatase, and β -glucosidase. Using the definition of soil health as the ability to promote plant growth, the influence of BPA on the growth and development of Zea mays , a plant used for energy production, was also tested. As well as the biomass of aerial parts and roots, the leaf greenness index (SPAD) of Zea mays was also assessed. A key aspect of the research was to identify those of the six remediating substances-molecular sieve, zeolite, sepiolite, starch, grass compost, and fermented bark-whose use could become common practice in both environmental protection and agriculture. Exposure to BPA revealed the highest sensitivity of dehydrogenases, urease, and acid phosphatase and the lowest sensitivity of alkaline phosphatase and catalase to this phenolic compound. The enzyme response generated a reduction in the biochemical fertility index (BA 21 ) of 64% (500 mg BPA) and 70% (1000 mg BPA kg -1 d.m. of soil). The toxicity of BPA led to a drastic reduction in root biomass and consequently in the aerial parts of Zea mays . Compost and molecular sieve proved to be the most effective in mitigating the negative effect of the xenobiotic on the parameters discussed. The results obtained are the first research step in the search for further substances with bioremediation potential against both soil and plants under BPA pressure.

Bisphenol A (BPA), with its wide array of products and applications, is currently one of the most commonly produced chemicals in the world. A narrow pool of data on BPA-microorganism-plant interaction mechanisms has stimulated the following research, the aim of which has been to determine the response of the soil microbiome and crop plants, as well as the activity of soil enzymes exposed to BPA pressure. A range of disturbances was assessed, based on the activity of seven soil enzymes, an abundance of five groups of microorganisms, and the structural diversity of the soil microbiome. The condition of the soil was verified by determining the values of the indices: colony development (CD), ecophysiological diversity (EP), the Shannon-Weaver index, and the Simpson index, tolerance of soil enzymes, microorganisms and plants (TI BPA ), biochemical soil fertility (BA 21 ), the ratio of the mass of aerial parts to the mass of plant roots (PR), and the leaf greenness index: Soil and Plant Analysis Development (SPAD). The data brought into sharp focus the adverse effects of BPA on the abundance and ecophysiological diversity of fungi. A change in the structural composition of bacteria was noted. Bisphenol A had a more beneficial effect on the Proteobacteria than on bacteria from the phyla Actinobacteria or Bacteroidetes . The microbiome of the soil exposed to BPA was numerously represented by bacteria from the genus Sphingomonas . In this object pool, the highest fungal OTU richness was achieved by the genus Penicillium , a representative of the phylum Ascomycota . A dose of 1000 mg BPA kg -1 d.m. of soil depressed the activity of dehydrogenases, urease, acid phosphatase and β -glucosidase, while increasing that of alkaline phosphatase and arylsulfatase. Spring oilseed rape and maize responded significantly negatively to the soil contamination with BPA.

The choice of the study objective was affected by numerous controversies and concerns around bisphenol F (BPF) and bisphenol S (BPS)-analogues of bisphenol A (BPA). The study focused on the determination and comparison of the scale of the BPA, BPF, and BPS impact on the soil microbiome and its enzymatic activity. The following parameters were determined in soil uncontaminated and contaminated with BPA, BPF, and BPS: the count of eleven groups of microorganisms, colony development (CD) index, microorganism ecophysiological diversity (EP) index, genetic diversity of bacteria and activity of dehydrogenases (Deh), urease (Ure), catalase (Cat), acid phosphatase (Pac), alkaline phosphatase (Pal), arylsulphatase (Aryl) and β -glucosidase (Glu). Bisphenols A, S and F significantly disrupted the soil homeostasis. BPF is regarded as the most toxic, followed by BPS and BPA. BPF and BPS reduced the abundance of Proteobacteria and Acidobacteria and increased that of Actinobacteria . Unique types of bacteria were identified as well as the characteristics of each bisphenol: Lysobacter, Steroidobacter, Variovorax, Mycoplana, for BPA, Caldilinea, Arthrobacter, Cellulosimicrobium and Promicromonospora for BPF and Dactylosporangium Geodermatophilus, Sphingopyxis for BPS. Considering the strength of a negative impact of bisphenols on the soil biochemical activity, they can be arranged as follows: BPS > BPF > BPA. Urease and arylsulphatase proved to be the most susceptible and dehydrogenases the least susceptible to bisphenols pressure, regardless of the study duration.

The concept of the study resulted from the lack of accurate data on the toxicity of bisphenol F (BPF) coinciding with the need for immediate changes in the global economic policy eliminating the effects of environmental contamination with bisphenol A (BPA). The aim of the experiment was to determine the scale of the previously unstudied inhibitory effect of BPF on soil biochemical activity. To this end, in a soil subjected to increasing BPF pressure at three contamination levels of 0, 5, 50 and 500 mg BPF kg -1 DM, responses of soil enzymes, dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase, arylsulphatase and β-glucosidase, were examined. Moreover, the study suggested a potentially effective way of biostimulating the soil by means of bioaugmentation with a consortium of four bacterial species: Pseudomonas umsongensis, Bacillus mycoides, Bacillus weihenstephanensis and Bacillus subtilis, and the following fungal species: Mucor circinelloides, Penicillium daleae, Penicillium chrysogenum and Aspergillus niger. It was found that BPF was a controversial BPA analogue due to the fact that it contributed to the inhibition of all the enzyme activities. Dehydrogenases proved to be the most sensitive to bisphenol contamination of the soil. The addition of 5 mg BPF kg -1 DM of soil triggered an escalation of the inhibition comparable to that for the other enzymes only after exposing them to the effects of 50 and 500 mg BPF kg -1 DM of soil. Moreover, BPF generated low activity of urease, acid phosphatase, alkaline phosphatase and β-glucosidase. Bacterial inoculum increased the activity of urease, β-glucosidase, catalase and alkaline phosphatase. Seventy-six percent of BPF underwent biodegradation during the 5 days of the study.

This study analysed the scale of bisphenol S (BPS) toxicity to the soil biochemical activity and is part of a wider effort to find solutions to restore the global soil environment balance, including elimination of the effects of ecosystem pollution with BPA, of which BPS is a significant analogue. However, since there has been no research on the effect of BPS on soil health, the objective of the study was pursued based on increasing the levels of soil contamination with the bisphenol 0, 5, 50 and 500 mg BPS kg -1 DM of soil and by observing the response of seven soil enzymes: dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase, arylsulphatase and β-glucosidase to the growing BPS pressure. The potential negative effect of bisphenol S was offset by bioaugmentation with a bacteria consortium-Pseudomonas umsongensis, Bacillus mycoides, Bacillus weihenstephanensis and Bacillus subtilis-and a fungi consortium Mucor circinelloides, Penicillium daleae, Penicillium chrysogenum and Aspergillus niger. BPS was found to be a significant inhibitor of the soil enzymatic activity and, in consequence, its fertility. Dehydrogenases and acid phosphatase proved to be the most susceptible to BPS pressure. Bioaugmentation with a bacteria consortium offset the negative effect of 500 mg BPS kg -1 DM of soil by inducing an increase in the activity of acid phosphatase and alkaline phosphatase, whereas the fungi consortium stimulated the activity of β-glucosidase and acid phosphatase. A spectacular dimension of bisphenol S inhibition corresponded with both the spring rape above-ground parts and root development disorders and the content of Ca and K in them. The BPS level in soil on day 5 of the experiment decreased by 61% and by another 19% on day 60.