1. Revealing mechanism of phenol-amine reaction to form humus in compost based on high-resolution liquid chromatography mass spectrometry and spectroscopy.
- Author
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Zhou J, Gao W, Xie L, Zhang R, Zhang Y, and Wei Z
- Subjects
- Soil chemistry, Phenols, Chromatography, Liquid methods, Liquid Chromatography-Mass Spectrometry, Humic Substances analysis, Amines chemistry, Composting methods, Mass Spectrometry methods, Phenol chemistry
- Abstract
Humus is the stable form of carbon storage in straw compost. The phenol-amine reaction is a pathway for humus formation in straw compost. In this study, two reaction systems, GP group (pyrogallol and glycine) and GCP group (catechol, pyrogallol, and glycine), were constructed in a simulated composting environment and revealed the molecular binding mechanism of the phenol-amine reaction through spectroscopy and mass spectrometry. The results showed that phenolic self-polymerization was faster than phenol-amine reaction. Therefore, the aromatization degree of GP was 27.14 % higher than that of GCP. The phenol-amine reaction first produced fulvic acid, and then formed humus units rich in active functional group structures (i.e., phenolic hydroxyl and carboxyl groups). These units further captured small molecule compounds to form humic acid eventually. This study would provide theoretical support for exploring the humus formation process and the promotion of straw humification by adding phenol or amino acids to compost., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Published
- 2024
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