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Start Over Author "Bailleul, Benjamin" Topic phaeodactylum tricornutum
4 results on '"Bailleul, Benjamin"'

1. Impaired photoprotection in Phaeodactylum tricornutum KEA3 mutants reveals the proton regulatory circuit of diatoms light acclimation.

Author

Seydoux, Claire, Storti, Mattia, Giovagnetti, Vasco, Matuszyńska, Anna, Guglielmino, Erika, Zhao, Xue, Giustini, Cécile, Pan, Yufang, Blommaert, Lander, Angulo, Jhoanell, Ruban, Alexander V., Hu, Hanhua, Bailleul, Benjamin, Courtois, Florence, Allorent, Guillaume, and Finazzi, Giovanni

Subjects
PHAEODACTYLUM tricornutum, DIATOMS, ACCLIMATIZATION, PROTONS, CALCIUM ions, CHARGE exchange
Abstract

Summary: Diatoms are successful phytoplankton clades able to acclimate to changing environmental conditions, including e.g. variable light intensity. Diatoms are outstanding at dissipating light energy exceeding the maximum photosynthetic electron transfer (PET) capacity via the nonphotochemical quenching (NPQ) process. While the molecular effectors of NPQ as well as the involvement of the proton motive force (PMF) in its regulation are known, the regulators of the PET/PMF relationship remain unidentified in diatoms.We generated mutants of the H+/K+ antiporter KEA3 in the model diatom Phaeodactylum tricornutum.Loss of KEA3 activity affects the PET/PMF coupling and NPQ responses at the onset of illumination, during transients and in steady‐state conditions. Thus, this antiporter is a main regulator of the PET/PMF coupling. Consistent with this conclusion, a parsimonious model including only two free components, KEA3 and the diadinoxanthin de‐epoxidase, describes most of the feedback loops between PET and NPQ.This simple regulatory system allows for efficient responses to fast (minutes) or slow (e.g. diel) changes in light environment, thanks to the presence of a regulatory calcium ion (Ca2+)‐binding domain in KEA3 modulating its activity. This circuit is likely tuned by the NPQ‐effector proteins, LHCXs, providing diatoms with the required flexibility to thrive in different ocean provinces. [ABSTRACT FROM AUTHOR]

Published
2022
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2. Multisignal control of expression of the LHCX protein family in the marine diatom Phaeodactylum tricornutum

Author

Taddei, Lucilla, Stella, Giulio Rocco, Rogato, Alessandra, Bailleul, Benjamin, Fortunato, Antonio Emidio, Annunziata, Rossella, Sanges, Remo, Thaler, Michael, Lepetit, Bernard, Lavaud, Johann, Jaubert, Marianne, Finazzi, Giovanni, Bouly, Jean Pierre, Falciatore, Angela, Biologie Computationnelle et Quantitative = Laboratory of Computational and Quantitative Biology (LCQB), Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Biotechnology [Verona] (UNIVR | DBT), University of Verona (UNIVR), Stazione Zoologica Anton Dohrn (SZN), Institute of Biosciences and Bioresources, CNR, Physiologie membranaire et moléculaire du chloroplaste (PMMC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Department of Plant Ecophysiology, University of Konstanz, Takuvik Joint International Laboratory ULAVAL-CNRS, Université Laval [Québec] (ULaval)-Centre National de la Recherche Scientifique (CNRS), Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Marie-Curie ITNs CALIPSO (ITN 2013 GA607607), AccliPhot (ITN 2012 GA 316427), Gordon and Betty Moore Foundation grant (GBMF 4966), Markus Teige, ANR-12-BIME-0005,DiaDomOil,Domestication des diatomées pour la production de biocarburants(2012), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Università degli studi di Verona = University of Verona (UNIVR), Université Laval [Québec] (ULaval)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Department of Biotechnology, University of Verona, Stazione Zoologica di Napoli (SZN), Stazione Zoologica di Napoli, Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC), Takuvik Joint ULaval/CNRS Laboratory, Centre National de la Recherche Scientifique (CNRS)-Laval University [Québec], Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0301 basic medicine, Physiology, [SDV]Life Sciences [q-bio], Light-Harvesting Protein Complexes, Plant Science, 01 natural sciences, Phaeodactylum tricornutum, Settore BIO/13 - Biologia Applicata, Photosynthesis, ComputingMilieux_MISCELLANEOUS, Gene expression regulation, biology, Non-photochemical quenching, marine diatom, Light acclimation, light, non-photochemical quenching, Research Paper, Signal Transduction, Protein family, Dark, gene expression, iron starvation, LHCX, nitrogen starvation, Algal Proteins, Diatoms, Phytoplankton, Gene Expression Regulation, 03 medical and health sciences, ddc:570, Botany, Gene family, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, 14. Life underwater, Marine diatoms, Nitrogen deficiency, Abiotic stress, Photoprotection, Protein, Iron deficiency, fungi, Non photochemical quenching, biology.organism_classification, 030104 developmental biology, Diatom, Light harvesting complex, Light stress, 010606 plant biology & botany
Abstract

Highlight Multiple stress signalling pathways regulate LHCX family gene expression in the diatom Phaeodactylum tricornutum to attune acclimation responses efficiently in highly variable ocean environments., Diatoms are phytoplanktonic organisms that grow successfully in the ocean where light conditions are highly variable. Studies of the molecular mechanisms of light acclimation in the marine diatom Phaeodactylum tricornutum show that carotenoid de-epoxidation enzymes and LHCX1, a member of the light-harvesting protein family, both contribute to dissipate excess light energy through non-photochemical quenching (NPQ). In this study, we investigate the role of the other members of the LHCX family in diatom stress responses. Our analysis of available genomic data shows that the presence of multiple LHCX genes is a conserved feature of diatom species living in different ecological niches. Moreover, an analysis of the levels of four P. tricornutum LHCX transcripts in relation to protein expression and photosynthetic activity indicates that LHCXs are differentially regulated under different light intensities and nutrient starvation, mostly modulating NPQ capacity. We conclude that multiple abiotic stress signals converge to regulate the LHCX content of cells, providing a way to fine-tune light harvesting and photoprotection. Moreover, our data indicate that the expansion of the LHCX gene family reflects functional diversification of its members which could benefit cells responding to highly variable ocean environments.

Published
2016

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