1. The common lavender (Lavandula angustifolia Mill.) pectic polysaccharides modulate phagocytic leukocytes and intestinal Peyer's patch cells.
- Author
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Georgiev YN, Paulsen BS, Kiyohara H, Ciz M, Ognyanov MH, Vasicek O, Rise F, Denev PN, Yamada H, Lojek A, Kussovski V, Barsett H, Krastanov AI, Yanakieva IZ, and Kratchanova MG
- Subjects
- Animals, Anti-Inflammatory Agents chemistry, Female, Humans, Mice, Mice, Inbred C3H, Nitric Oxide metabolism, Polysaccharides, RAW 264.7 Cells, Rabbits, Reactive Oxygen Species metabolism, Sheep, Flowers chemistry, Lavandula chemistry, Macrophages drug effects, Pectins pharmacology, Peyer's Patches cytology
- Abstract
Two pectic (chPS-L1, chPS-L2) and one polyphenolic (chPP-L) fractions were obtained from lavender flowers after boiling water extraction, exhaustive removing of alcohol-soluble molecules and SEC. chPS-L1 (52.4kDa) contains mainly low-acetylated and high-methoxylated homogalacturonans (HG), and smaller rhamnogalacturonan (RG) I backbone fragments rich in 1,3,5-branched arabinan and arabinogalactan (AG) II side chains. chPS-L2 (21.8kDa) contains predominantly similarly esterified HG, followed by RGI with AGII structures and RGII. The prevalence of catechin and epicatechin in chPP-L indicates that they form weak interactions with pectins. chPS-L1 and chPS-L2 enhanced ß
2 -integrin expression on neutrophils, inducing ROS generation and macrophage NO production. Both the effects on ß2 -integrin and high complement fixation activity of chPS-L1 were proposed for its inhibitory action against PMA- and OZP-activated ROS formation. This, together with suppression of NO generation after co-stimulation with chPS-L1 and LPS, suggested anti-inflammatory activity of studied pectins. Lavender polysaccharides expressed intestinal Peyer's patch immunomodulating activity., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2017
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