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In this study, we conducted a meta-analysis of soil microbial communities at three, pilot-scale field sites simulating shallow infiltration for managed aquifer recharge (MAR). We evaluated shifts in microbial communities after infiltration across site location, through different soils, with and without carbon-rich amendments added to test plots. Our meta-analysis aims to enable more effective MAR basin design by identifying potentially important interactions between soil physical–geochemical parameters and microbial communities across several geographically separate MAR basins. We hypothesized infiltration and carbon amendments would lead to common changes in subsurface microbial communities at multiple field sites but instead found distinct differences. Sites with coarser (mainly sandy) soil had large changes in diversity and taxa abundance, while sites with finer soils had fewer significant changes in genera, despite having the greatest increase in nitrogen cycling. Below test plots amended with a carbon-rich permeable reactive barrier, we observed more nitrate removal and a decrease in genera capable of nitrification. Multivariate statistics determined that the soil texture (a proxy for numerous soil characteristics) was the main determinant of whether the microbial community composition changed because of infiltration. These results suggest that microbial communities in sandy soil with carbon-rich amendments are most impacted by infiltration. Soil composition is a critical parameter that links between microbial communities and nutrient cycling during infiltration and could influence the citing and operation of MAR to benefit water quality and supply. [ABSTRACT FROM AUTHOR]

Abstract We present results from field experiments linking hydrology, geochemistry, and microbiology during infiltration at a field site that is used for managed aquifer recharge (MAR). These experiments measured how a horizontal permeable reactive barrier (PRB) made of woodchips impacted subsurface nitrate removal and microbial ecology. Concentrations of dissolved organic carbon consistently increased in infiltrating water below the PRB, but not in un-amended native soil. The average nitrate removal rate in soils below the PRB was 1.5 g/m2/day NO 3 -N, despite rapid infiltration (up to 1.9 m/d) and a short fluid residence time within the woodchips (≤6 h). In contrast, 0.09 g/m2/day NO 3 -N was removed on average in native soil. Residual nitrate in infiltrating water below the PRB was enriched in δ15N and δ18O, with low and variable isotopic enrichment factors that are consistent with denitrification during rapid infiltration. Many putative denitrifying bacteria were significantly enhanced in the soil below a PRB; Methylotenera mobilis and genera Microbacterium , Polaromonas , and Novosphingobium had log 2 fold-changes of +4.9, +5.6, +7.2, and +11.8, respectively. These bacteria were present before infiltration and were not enhanced in native soil. It appears that the woodchip PRB contributed to favorable conditions in the underlying soil for enhanced nitrate removal, quantitatively shifting soil microbial ecology. These results suggest that using a horizontal PRB could improve water quality during rapid infiltration for MAR. Graphical abstract Image 1 Highlights • A horizontal permeable reactive barrier (PRB) was tested for impacts on N-cycling. • The PRB was associated with enhanced nitrate removal during rapid infiltration. • Putative denitrifying microbial clades were enhanced in soils below a PRB. • Geochemical, isotopic, and microbial data are consistent with denitrification. • Using a horizontal woodchip PRB could improve water quality during managed recharge. [ABSTRACT FROM AUTHOR]

• Soil carbon amendments decreased nitrate loads during infiltration. • Almond shells promoted more nitrate removal than wood mulch or native soil. • Almond shells mobilized more geogenic trace metals than wood mulch or soil. • Higher infiltration rates were associated with less nitrate removal. • Infiltration through carbon amendments changed microbial ecology in soil. We present results from a series of laboratory column studies investigating the impacts of infiltration dynamics and the addition of a soil-carbon amendment (wood mulch or almond shells) on water quality during infiltration for flood-managed aquifer recharge (flood-MAR). Recent studies suggest that nitrate removal could be enhanced during infiltration for MAR through the application of a wood chip permeable reactive barrier (PRB). However, less is understood about how other readily available carbon sources, such as almond shells, could be used as a PRB material, and how carbon amendments could impact other solutes, such as trace metals. Here we show that the presence of a carbon amendment increases nitrate removal relative to native soil, and that there is greater nitrate removal in association with longer fluid retention times (slower infiltration rates). Almond shells promoted more efficient nitrate removal than wood mulch or native soil, but also promoted the mobilization of geogenic trace metals (Mn, Fe, and As) during experiments. Almond shells in a PRB likely enhanced nitrate removal and trace metal cycling by releasing labile carbon, promoting reducing conditions, and providing habitat for microbial communities, the composition of which shifted in response. These results suggest that limiting the amount of bioavailable carbon released by a carbon-rich PRB may be preferred where geogenic trace metals are common in soils. Given the dual threats to groundwater supplies and quality worldwide, incorporating a suitable carbon source into the soil for managed infiltration projects could help to generate co-benefits and avoid undesirable results. [Display omitted] [ABSTRACT FROM AUTHOR]