1. Cultures and co-cultures of human blood mononuclear cells and endothelial cells for the biocompatibility assessment of surface modified AISI 316L austenitic stainless steel
- Author
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Maria Stio, Cristina Treves, Francesca Borgioli, and Maria Martinesi
- Subjects
0301 basic medicine ,Materials science ,Biocompatibility ,Nitrogen ,Surface Properties ,Biocompatible Materials ,Enzyme-Linked Immunosorbent Assay ,Bioengineering ,02 engineering and technology ,engineering.material ,Matrix metalloproteinase ,Peripheral blood mononuclear cell ,Collagen Type I ,Umbilical vein ,Biomaterials ,03 medical and health sciences ,X-Ray Diffraction ,Human Umbilical Vein Endothelial Cells ,Humans ,Austenitic stainless steel ,Cells, Cultured ,Cell Proliferation ,Chromatography ,Metallurgy ,Intercellular Adhesion Molecule-1 ,Stainless Steel ,021001 nanoscience & nanotechnology ,Coculture Techniques ,Matrix Metalloproteinases ,In vitro ,Corrosion ,030104 developmental biology ,Mechanics of Materials ,Cell culture ,Culture Media, Conditioned ,Dielectric Spectroscopy ,Leukocytes, Mononuclear ,engineering ,Cytokines ,0210 nano-technology ,Nitriding - Abstract
Samples of AISI 316L austenitic stainless steel were subjected either to grinding and polishing procedure, or to grinding and then low temperature glow-discharge nitriding treatment, or to grinding, nitriding and subsequently coating with collagen-I. Nitrided samples, even if only ground, show a higher corrosion resistance in PBS solution, in comparison with ground and polished AISI 316L. Biocompatibility was evaluated in vitro by incubating the samples with either peripheral blood mononuclear cells (PBMC) or human umbilical vein endothelial cells (HUVEC), tested separately or in co-culture. HUVEC-PBMC co-culture and co-incubation of HUVEC with PBMC culture medium, after the previous incubation of PBMC with metallic samples, allowed to determine whether the incubation of PBMC with the different samples might affect HUVEC behaviour. Many biological parameters were considered: cell proliferation, release of cytokines, matrix metalloproteinases (MMPs) and sICAM-1, gelatinolytic activity of MMPs, and ICAM-1 protein expression. Nitriding treatment, with or without collagen coating of the samples, is able to ameliorate some of the biological parameters taken into account. The obtained results point out that biocompatibility may be successfully tested in vitro, using cultures of normal human cells, as blood and endothelial cells, but more than one cell line should be used, separately or in co-culture, and different parameters should be determined, in particular those correlated with inflammatory phenomena.
- Published
- 2016
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