1. Membrane-based preparative methods and bioactivities mapping of tempe-based peptides.
- Author
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Sitanggang AB, Lesmana M, and Budijanto S
- Subjects
- Amino Acid Sequence, Angiotensin-Converting Enzyme Inhibitors chemistry, Angiotensin-Converting Enzyme Inhibitors metabolism, Antioxidants chemistry, Chromatography, High Pressure Liquid, Databases, Protein, Electrophoresis, Polyacrylamide Gel, Fermentation, Filtration, Flour analysis, Hydrolysis, Mass Spectrometry, Papain metabolism, Peptides metabolism, Peptidyl-Dipeptidase A chemistry, Peptidyl-Dipeptidase A metabolism, Solubility, Glycine max metabolism, Subtilisins metabolism, Peptides chemistry, Soy Foods analysis
- Abstract
This study was aimed to produce bioactive peptides from optimally fermented tempe, and map their overall bioactivities. There were three preparative methods utilized for producing tempe-based peptides, such as water-facilitated extraction, alcalase, and papain hydrolysis, and in combination with membrane filtration. Fermenting soybean at 144 h was selected as the optimum time based on protein content and degree of hydrolysis. Through SDS-PAGE analysis, an increased degree of hydrolysis with longer fermentation time was confirmed. The best preparative method for producing bioactive peptides was through papain hydrolysis and followed by 5 kDa membrane filtration. By this, the enhancement was distinct for antioxidant activity, ACE-, α-glucosidase-, and Kunitz trypsin-inhibitory activity. The annotated peptide sequences resulting from Nano LC Ultimate 3000 Series System tandem Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer were matched with the BIOPEP database. The major bioactivities of tempe peptides obtained were as an ACE inhibitor, antioxidant, and antithrombotic., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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