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The experiment was conducted to investigate the effect of shikimic acid on growth performance, diarrhea index, serum immunity and antioxidant indicators of piglets infected with porcine epidemic diarrhea virus (PEDV). A total of 120 clinically confirmed PEDV infected seven-day-old piglets (Duroc × Landrace × Yorkshire) were randomly divided into six groups, with five replicates in each group and four pigs in each replicate. Treatment group 1, group 2 and group 3 were administered 800, 400 and 200 mg/kg of shikimic acid daily to piglets infected with PEDV, treatment group 4 were treated with daily administration of 400 mg/kg chlorogenic acid to piglets infected with PEDV, treatment group 5 were treated with 20 000 units of gentamicin per day to piglets infected with PEDV, treatment group 6 were treated as positive control groups, and another 20 healthy piglets were selected as the blank control group. The experiment lasted for 5 d, and then observed for 3 d. The results showed that compared with control group with positive PEDV infection, the ADG of piglets with treatment group 1, group 2 and group 3 was significantly increased (P<0.05). The cure rate of PEDV infection in treatment group 1 and group 2 reached 85%, and the cure rate in treatment group 5 was 65%. The IgG content in the serum of piglets in group 1, group 2 and group 3 was significantly increased (P<0.05). The activity of SOD, CAT, NO, MPO, GSH-Px and T-AOC in the serum of piglets in group 1, group 2 and group 3 were significantly increased (P<0.05), and the content of MDA and XOD was significantly reduced (P<0.05). The experiment indicates that shikimic acid can improve the growth performance of piglets, reduce diarrhea index, enhance immunity and serum antioxidant capacity, and have a disease resistance and growth promoting effect on PEDV infected piglets. [ABSTRACT FROM AUTHOR]

In order to establish a rapid multiplex RT-PCR assay for detection and differentiation of variant，classical and attenuated vaccine strains of porcine epidemic diarrhea virus (PEDV). Two pairs of specific primers were designed based on S gene sequences and 0RF3 gene sequences of variant，classical and attenuated vaccine strains PEDV published in GenBank，to amplify the partial S gene fragment and ORF3 gene fragment of different PEDV strains，the type of PEDV strain could be differentiated according to the number of the fragment and the size of the fragment. Results showed that the multiplex RT-PCR could detect and differentiate variant， classical and attenuated vaccine strains PEDV. Variant PEDV strain generated two fragments，they were 826 bp of the S gene and 234 bp of the ORF3 gene，respectively. Classical PEDV strain only amplified 234 bp of the ORF3 gene，while attenuated vaccine strain produced 185 bp of the ORF3 gene. The multiplex RT-PCR did not cross-react with TGEV，PRoVA，PKV，PRRSV，PRV，CSFV，PCV-2，JEV，TTV and PPV used in the study. Testing of the sensitivity of RT-PCR indicated as low as 2. 3 x 10 -4 ng/μl nuclear acids could be detected accurately and rapidly. Ninety-one stool specimens collected from different farms in Guangxi Province were detected by the established multiplex RT-PCR，64 samples were positive for PEDV， of which 89. 06 % (57/64 ) was variant PED V strains，4. 69 % (3/64) was classical PEDV strain and 6. 25 % (4/64) was attenuated vaccine PEDV strain. Therefore the multiplex RT-PCR could be used as an effective tool for differentiating diagnosis of PEDV in epidemiological investigations. [ABSTRACT FROM AUTHOR]