Peatland ecosystems in the eastern Georgian Bay, Ontario, region often provide overwintering habitat for the eastern massasauga rattlesnake (Sistrurus c. catenatus), a species considered at-risk across its range. Suitable overwintering habitat requires a resilience zone with peat temperatures above 0°C and a water table position sufficient to provide moisture without risk of flooding and these ecohydrological conditions commonly occur in raised peatland microforms (hummocks). Due to a changing climate, these peatlands are at risk of increased wildfire frequency and burn severity which may threaten overwintering habitat availability and suitability. In 2018, a wildfire burned over 11,000 ha of the eastern Georgian Bay landscape which serves as critical habitat for the massasauga. We monitored water table position, snow depth, rainfall, and peat thermal dynamics in hummocks in three burned and three unburned peatlands to assess the potential impacts of wildfire on massasauga overwintering habitat. We found that hummocks were able to provide unfrozen and unflooded habitat regardless of peat burn severity and that surface complexity and peatland-scale characteristics provided the greatest control on microhabitat suitability. This research highlights the importance of conserving peatland ecosystems that provide resilient species at risk habitat. Thesis Master of Science (MSc) The eastern massasauga rattlesnake is a species at risk native to Ontario and parts of the USA. In the eastern Georgian Bay region, massasaugas overwinter in wetlands for up to half the year. This is a sensitive period because flooding or freezing within the hibernacula can be fatal. Due in part to climate change, wetlands in this region are at increased wildfire risk which may threaten the quality of massasauga overwintering habitat. In 2018, a wildfire burned over 11,000 ha of land along eastern Georgian Bay, some of which was massasauga habitat. We monitored the water table position and soil temperature in potential massasauga overwintering habitat to assess its quality after wildfire. We found that wetlands provide unflooded and unfrozen habitat even when burned, and that wetland surface complexity is likely an important regulator of overwintering habitat quality. This research highlights the importance of identifying and protecting wetland ecosystems that provide resilient habitat in the face of a disturbance.