Electromyography (EMG) signal is a useful signal in various medical and engineering applications. To extract the useful information in the EMG signal, feature extraction method should be performed. The extracted features of the EMG signal are usually calculated based on linear or statistical methods, but the EMG signal exhibits the nonlinear and more complex in the properties. With recent advances in nonlinear analysis we are proposing the study of the EMG signals from upper-limb movements using Detrended Fluctuation Analysis (DFA) method. This study used EMG signals obtained from eight upper-limb movements and five muscle positions as representative EMG signals. The usefulness of the DFA method has been proposed to discriminate the upper-limb movements. Complete comparative studies of an optimal parameter of the DFA method were performed. From the viewpoints of maximum class separability, robustness, and complexity, scaling exponent obtained from the DFA method shows the appropriateness to be used as a feature in the classification of the EMG signal. From the experimental results, an optimal DFA method is obtained under these conditions: the minimum box size is approximately four, the maximum box size is one-tenth of the signal length, the box size increment is based on a power of two, and the quadratic polynomial fits is used in the fitting procedure. Moreover, the classification performance of the DFA method is better than other existing nonlinear methods, including the Higuchi's method. [ABSTRACT FROM AUTHOR]