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ABSTRACT Integrated crop-livestock systems (ICLS) foster synergistic relationships to increase nitrogen (N) cycling and soil organic carbon (SOC) accrual in agricultural setups. This study evaluated how the grazing intensity and N fertilization (rates and timing) affect both SOC and N fractions, and soil organic matter chemical composition in an ICLS managed under no-tillage in an Oxisol, six years after initiation. The ICLS was compared to a nearby pasture (PA) and a native forest (NF). The treatments consisted of two grazing intensities: Low Sward Height (LH) and High Sward Height (HH) were maintained with high and low stocking rates, respectively. The HH varied between 0.20 and 0.60 m, and LH between 0.10 and 0.30 m according to the plant forage species throughout the experiment. Fertilization using 200 kg ha-1 N-urea, not splitting up, was conducted at two timings, either at the winter pasture establishment (autumn), about 35 days after sowing or during the summer cash crop cycle (spring). Total N amount per year, including both phases, pasture and cash crop was the same for all treatments. The SOC and N contents were assessed in soil and particulate organic matter (POM), while carbon (C) and N stocks were specifically determined in the soil. Soil organic matter composition was characterized by FTIR. The combination of HH and N fertilization during the pasture phase increased the content of C from 36.1 to 39.9 ± 0.7 g C kg-1 and of N from 2.7 to 3.2 ± 0.1 g N kg-1. The SOC stocks varied from 37.3 to 41.1 ± 0.7 Mg C ha-1, and the N stocks from 2.1 to 3.3 ± 0.1 Mg N ha-1 at 0.0-0.10 m soil layer. The SOC content of the POM and the soil organic matter chemical composition determined by FTIR were mainly affected by the grazing intensity. The HH led to an increased in C content within the POM fraction, reaching values of 51.6 ± 1 and 49.2 g C kg-1, respectively to N crop fertilization and N pasture fertilization. Land-use changed how organic functional groups were stored in soil organic matter fractions. The NF had a greater abundance of aliphatic and phenol in the MAOM, while pasture and ICLS systems had greater aliphatic in the POM fraction. In ICLS, SOC accrual was positively associated with more recalcitrant organic functional groups of phenol, aromatic, and carbonyl C-O. The HH increases SOC accrual, while N-fertilization on pasture ensures adequate nutrition of plants and animals during the winter ICLS phase, at the same time as providing greater residual N for subsequent cash crops through enhanced catalyzed by ruminants. Therefore, grazing and fertilization management strategies should be considered to promote sustainable agriculture intensification with ICLS.