Abstract: The contribution presents the application of proton induced X-ray emission (PIXE), as a non-destructive analytical method, to the identification and study of historic paper, parchment and textile objects. The results show the possibilities for identification of inks, pigments, dyes, surface layers and inorganic impurities in historic objects made of organic materials. The measurement of elemental concentrations in these materials provides a sound basis for investigation of their composition and of degradation processes. PIXE has been found useful in evaluating the viability of a range of potential conservation treatments by measuring the elemental changes before and after the process has been carried out. Like any other form of analysis, the PIXE method has its limitations, but its great strength is in its non-destructiveness, high sensitivity and accuracy in determining composition and concentration distribution over a wide range of chemical elements. For objects made of complex organic materials the information obtained should be interpreted in combination with other analytical methods. [Copyright &y& Elsevier]