Search

Your search keyword '"Shen, Yuting"' showing total 4 results
Start Over Author "Shen, Yuting" Topic oxygen
4 results on '"Shen, Yuting"'

3. Kinetic analysis of sodium gluconate production by Aspergillus niger with different inlet oxygen concentrations

Author

Ju Chu, Haifeng Hang, Shen Yuting, Yingping Zhuang, Xiwei Tian, and Wei Zhao

Subjects
0106 biological sciences, 0301 basic medicine, 030106 microbiology, Kinetics, Analytical chemistry, chemistry.chemical_element, Bioengineering, Gluconates, Models, Biological, 01 natural sciences, Oxygen, Industrial Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Bioreactors, Exponential growth, 010608 biotechnology, Biomass, Sodium gluconate, biology, Aspergillus niger, Substrate (chemistry), General Medicine, biology.organism_classification, Glucose, Logistic Models, chemistry, Yield (chemistry), Fermentation, Limiting oxygen concentration, Biotechnology
Abstract

To further understand fermentation kinetics of sodium gluconate (SG) production by Aspergillus niger with different inlet oxygen concentrations, logistic model for cell growth and two-step models for SG production and glucose consumption were established. The results demonstrated that the maximum specific growth rate (µm) presented exponential relationship with inlet oxygen concentration and the maximum biomass (Xm) exhibited linear increase. In terms of SG production, two-step model with Luedeking-Piret equation during growth phase and oxygen-dependent equation during stationary phase could well fit the experimental data. Notably, high inlet oxygen concentration exponentially improved SG yield (YP/S), whereas biomass yield to glucose (YX/S) and cell maintenance coefficient (m) were almost independent on inlet oxygen concentration, indicating that high oxygen supply enhancing SG synthesis not only functioning as a substrate directly, but also regulating glucose metabolism towards SG formation. Finally, the applicability and predictability of the proposed models were further validated by additional experiments.

Published
2018

4. Oxygen-enriched fermentation of sodium gluconate by Aspergillus niger and its impact on intracellular metabolic flux distributions.

Author

Shen, Yuting, Tian, Xiwei, Zhao, Wei, Hang, Haifeng, and Chu, Ju

Abstract

Different concentrations of oxygen-enriched air were utilized for sodium gluconate (SG) fermentation by Aspergillus niger. The fermentation time shortened from 20 to 15.5 h due to the increase of volumetric oxygen transfer coefficient (Ka) and the formation of more dispersed mycelia when inlet oxygen concentration ascended from 21 to 32%. According to metabolic flux analysis, during the growth phase, extracellular glucose for SG synthesis accounted for 79.0 and 85.3% with air and oxygen-enriched air (25%), respectively, whereas the proportions were 89.4 and 93.0% in the stationary phase. Intracellular glucose consumption decreased in oxygen-enriched fermentation, as cell respiration was more high-efficiently performed. Metabolic profiling indicated that most intermediates in TCA cycle and EMP pathway had smaller pool sizes in oxygen-enriched fermentations. Moreover, the main by-product of citric acid dramatically decreased from 1.36 to 0.34 g L in oxygen-enriched fermentation. And the sodium gluconate yield increased from 0.856 to 0.903 mol mol. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results