1. Derepression in Saccharomyces cerevisiae can be dissociated from cellular proliferation and deoxyribonucleic acid synthesis.
- Author
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Mahler HR, Assimos K, and Lin CC
- Subjects
- Bacterial Proteins biosynthesis, Cell Membrane enzymology, Cytochrome Reductases biosynthesis, DNA, Mitochondrial biosynthesis, Enzyme Repression, Glutamate Dehydrogenase biosynthesis, Kinetics, Malate Dehydrogenase biosynthesis, Mitochondria enzymology, Mutation, NADH, NADPH Oxidoreductases biosynthesis, Saccharomyces cerevisiae growth & development, DNA, Bacterial biosynthesis, Genes, Oxidoreductases biosynthesis, Saccharomyces cerevisiae enzymology
- Abstract
A method has been developed that permits precise control of release from catabolite repression in Saccharomyces cerevisiae. It consists of transferring cells growing exponentially on 5% glucose to derepression medium at high cell density. Derepression then proceeds with reproducible kinetics and is complete within 6 to 7.5 h for various intra- and extramitochondrial markers, in the absence of any substantial increase in cellular dry weight or protein. Nuclear (and mitochondrial) deoxyribonucleic acid synthesis can be interrupted in certain thermosensitive (cdc) mutants at the nonpermissive temperature; a shift to this temperature before the onset of derepression has no effect on its outcome.
- Published
- 1975
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