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The present study was performed to determine the acute toxicity of sodium laureth sulfate (SLES) and its sublethal effects on oxidative stress enzymes in benthic oligochaete worm Tubifex tubifex. The results showed that 96 h median lethal concentration (LC 50 ) value of SLES for Tubifex tubifex is 21.68 mg/l. Moreover exposed worms showed abnormal behaviours including incremented erratic movement, mucus secretion, and decreased clumping tendency at acute level. Percentage of autotomy additionally increased significantly (P < 0.05) with the increasing dose of toxicant at 96 h exposure. Sublethal concentrations of SLES (10% and 30% of 96 h LC 50 value) caused paramount alterations in the oxidative stress enzymes. Superoxide dismutase (SOD), reduced glutathione (GSH), glutathione S-transferase (GST), and glutathione peroxidase (GPx) exhibited a striking initiatory increment followed by a resulting descending pattern. Moreover, during exposure times, catalase (CAT) activity and malondialdehyde (MDA) level increased markedly with incrementing concentrations of SLES. However, the effects of sodium laureth sulfate on Tubifex tubifex were characterized and portrayed by the development of a correlation matrix and an integrated biomarker response (IBR) assessment. These results indicate that exposure to this anionic surfactant alters the survivability and behavioral response at acute level and modifies changes in oxidative stress enzymes at sublethal level in Tubifex tubifex., (Copyright © 2021. Published by Elsevier Inc.)

The present study was aimed to assess the acute toxicity of organophosphate pesticide, profenofos; synthetic pyrethroid pesticide, λ cyhalothrin and biopesticide, azadirachtin and their sublethal effects on growth rate and oxidative stress biomarkers in Tubifex tubifex in vivo. The results showed that 96 h LC 50 value of profenofos, λ cyhalothrin and azadirachtin to Tubifex tubifex are 0.59, 0.13 and 82.15 mg L -1 respectively. Pesticide treated worms showed several behavioral abnormalities including increased mucus secretion, erratic movements, wrinkling activity and decreased clumping tendency during acute exposure. The percentage of autotomy increased significantly (p < 0.05) with the increasing concentration of the pesticides at 96 h of exposure. Sublethal concentrations of profenofos (0.059 and 0.118 mg L -1 ), λ cyhalothrin (0.013 and 0.026 mg L -1 ) and azadirachtin (8.2 and 16.4 mg L -1 ) caused significant alterations in growth rate and oxidative stress enzymes in T. tubifex during 14 days exposure period. The growth rate of the pesticide exposed worms decreased significantly (P < 0.05) in a concentration and duration-dependent manner. Superoxide dismutase (SOD), reduced glutathione (GSH), glutathione-s-transferase (GST) and glutathione peroxidase (GPx) demonstrated a noteworthy (p < 0.05) initial induction followed by a subsequent reduction, while catalase (CAT) and malondialdehyde (MDA) exhibited noteworthy induction (p < 0.05) all through the exposure time. Through principal component analysis, correlation matrix, and integrated biomarker response, the effects of profenofos, λ cyhalothrin and azadirachtin on T. tubifex were distinguished. These results indicate that exposure to profenofos, λ cyhalothrin and azadirachtin affect survivability, change the behavioral responses, reduce the growth rate and induce oxidative stress enzymes in T. tubifex., (Copyright © 2020 Elsevier Inc. All rights reserved.)

The present study was assessed to determine the in vivo toxic effects of a cationic surfactant, cetylpyridinium chloride (CPC), and an anionic surfactant, sodium dodecyl sulfate (SDS) in terms of oxidative stress biomarkers in benthic oligochaete worm Tubifex tubifex for 14 days. The investigation demonstrated that sublethal concentrations of CPC (0.0213, and 0.0639 mg L -1 ) and SDS (1.094 and 3.092 mg L -1 )induced paramount alterations in the oxidative stress enzymes in Tubifex tubifex. Superoxide dismutase (SOD), glutathione S-transferase (GST), reduced glutathione (GSH), and glutathione peroxidase (GPx) exhibited an initial notable increase in their activities in the surfactants exposed worms at 1d and 7d of exposure period followed by consequential reduction at 14d exposure period with respect to control, while catalase (CAT) and malondialdehyde (MDA) activities markedly incremented gradually throughout the exposure periods. Through the construction of the correlation matrix and integrated biomarker response (IBR), the effects of CPC and SDS on Tubifex tubifex were distinguished. These results indicate that exposure to these cationic and anionic surfactants modulates the levels of oxidative stress enzymes in Tubifex tubifex., (Copyright © 2020 Elsevier Inc. All rights reserved.)

The present invivo study was designed to elucidate the toxic effect of lead on oxidative stress, Na(+)K(+)ATPase and mitochondrial electron transport chain activity of the brain of Clarias batrachus. The fish were exposed to 10 and 20% of the derived 96 h LC(50) value, 37.8 and 75.6 mg L(-1), respectively, and sampled on 20, 40 and 60 days. Exposure of fish brain to lead demonstrated an increased production of reactive oxygen species, increased lipid peroxidation, loss of protein thiol groups in synaptosomal fraction with the decreased activity of Na(+)K(+)ATPase, partial inactivation of mitochondrial electron transport chain activity and energy depletion. However, no change in protein carbonyl content in synaptosomal fraction was observed due to lead exposure. Concluding the results of our investigation we suggest that lead exposure induces oxidative stress in the brain of Clarias batrachus and the decline in Na(+)K(+)ATPase activity was presumeably mediated by the combined action of lipid peroxidation and deficient mitochondrial electron transport chain activity.

Our current study examines the significant impact of aniline on the fish feed freshwater benthic annelid, Tubifex tubifex, including mortality, changes in total protein concentration and antioxidant enzyme activity (catalase, superoxide dismutase, glutathione-s transferase and glutathione peroxidase), as well as the generation of oxidative stress (by assessing malondialdehyde concentration). The 96-hour LC50 value of aniline is 276.940 mg/L. The General Unified Threshold Model of Survival (GUTS) was used to validate the survival rate pattern of the test organism in response to aniline at the acute level, with the GUTS-SD model better correctly predicting the expected course of population death. During long-term bioassay antioxidant enzymes activities (superoxide dismutase, glutathione-s transferase and glutathione peroxidase) and oxidative stress biomarkers concentration (malondialdehyde concentration) is substantially enhanced while protein concentration and Catalase activity are markedly reduced during the bioassay. The stress and detrimental effects of aniline on Tubifex tubifex were examined in the current study, taking into account all biomarkers, by utilising the Integrated Biomarker Response index. The results show that exposure to toxicants like aniline has a detrimental effect on Tubifex tubifex's survival rate and general health. [ABSTRACT FROM AUTHOR]

This study aims to evaluate the toxic effects of organophosphate insecticide triazophos, biopesticide spinosad, and a pyrethroid insecticide cypermethrin on benthic Oligochaete worm, Branchiura sowerbyi during 96 h acute exposure. B. sowerbyi were exposed to two different sub-lethal concentrations (10% and 50% of 96h LC50) of triazophos, spinosad, and cypermethrin for 96 h in laboratory conditions. Catalase (CAT) activity of the control and treated worms were evaluated after 24 and 96 h of exposure. Integrated biomarker response (IBR) was applied for comparison between these three toxicants. For all sub-lethal doses i.e. 2.25 mg.L-1 and 0.5 mg.L-1 of test chemical triazophos, 3.07 mg.L-1 and 0.6 mg.L-1 of test chemical spinosad, and 0.38 mg.L-1 and 0.08 mg.L-1 of test chemical cypermethrin, catalase (CAT) activity raised significantly (p<0.05) in the treated worms in compare to the control worms. This study shows that toxicants including Triazophos, spinosad, and cypermethrin cause a large increase in catalase (CAT) activity in Branchiura sowerbyi, which is likely due to the toxicant's increased ROS creation neutralizing the negative effects. IBR analysis aids in the differentiation of these three compounds' harmful effects. As per IBR analysis, the rank of the toxicants is Cypermethrin > Triazophos > Spinosad. [ABSTRACT FROM AUTHOR]

The current study aimed to determine the detrimental effects of sodium dodecyl sulphate (SDS), cetylpyridinium chloride (CPC), and sodium laureth sulphate (SLES) on survival rate and oxidative stress enzymes in Branchiura sowerbyi (Beddard, 1892). The 96 h LC50 values of SDS, CPC and SLES to Branchiura sowerbyi were estimated to be 8.27, 0.12 and 19.58 mg/L, respectively. Moreover, surfactant toxicity was projected using general threshold survival models (GUTS) in terms of required data sets and fit performance. The result suggested that GUTS-T (individual tolerance) model can better predict the survival rate observed in Branchiura sowerbyi for surfactant exposure than the GUTS-SD (stochastic death) model. Sublethal levels of SDS, CPC and SLES (10% and 30% of the 96 h LC50 value) elicited substantial changes in oxidative stress enzymes over the 14-day exposure period. Superoxide dismutase (SOD), reduced glutathione (GSH), glutathione S-transferase (GST) and glutathione peroxidase (GPx) demonstrated a significant increasing trend initially followed by a subsequent declining trend, but the levels of catalase (CAT), as well as malondialdehyde (MDA), increased significantly throughout the periods of exposure. The consequences of SDS, CPC and SLES on Branchiura sowerbyi were evaluated using a correlation matrix, principal component analysis, and integrated biomarker response assessment. These findings suggest that SDS, CPC and SLES exposure detrimentally affect survival rate and alter the levels of oxidative stress enzymes in Branchiura sowerbyi. [ABSTRACT FROM AUTHOR]

In response to variation in abiotic factors and metal accumulation pattern in fish, current research tries to draw a chronological relationship between the annual profiles of oxidative stress markers. Rhinomugil corsula were collected twice a month over the course of an annual cycle from two separate locations. Fluctuations in concentrations of certain metals (lead, zinc, copper and cadmium) in water and gill tissues were estimated accordingly. Current study also measured the rhythmic expression of antioxidants (enzymatic and non-enzymatic) and Na+-K+ATPase in gill tissues. Therefore, present study explored rhythmic responses of oxidative stress marker to assess the impact of different metals on selected fish species. Here, we tried to develop a conceptual idea to analyse and predict the consequences of metal pollution on the possible shift in rhythmicity of aforesaid antioxidants in fish gill. Our results indicate that the amplitude of circannual rhythms of all the selected stress markers varied accordingly with an increase in metal accumulation throughout an annual cycle. A shift in the pattern of annual rhythmicity of antioxidant markers is noted, associated with the fluctuation in the accumulation of zinc and copper. Therefore, current information would help to frame responses of fish gill in metal infected aquatic system. [ABSTRACT FROM AUTHOR]

The present invivo study was designed to elucidate the toxic effect of lead on oxidative stress, Na+K+ATPase and mitochondrial electron transport chain activity of the brain of Clarias batrachus. The fish were exposed to 10 and 20% of the derived 96 h LC50 value, 37.8 and 75.6 mg L−1, respectively, and sampled on 20, 40 and 60 days. Exposure of fish brain to lead demonstrated an increased production of reactive oxygen species, increased lipid peroxidation, loss of protein thiol groups in synaptosomal fraction with the decreased activity of Na+K+ATPase, partial inactivation of mitochondrial electron transport chain activity and energy depletion. However, no change in protein carbonyl content in synaptosomal fraction was observed due to lead exposure. Concluding the results of our investigation we suggest that lead exposure induces oxidative stress in the brain of Clarias batrachus and the decline in Na+K+ATPase activity was presumeably mediated by the combined action of lipid peroxidation and deficient mitochondrial electron transport chain activity. [ABSTRACT FROM AUTHOR]

This study aimed to understand the effects of freshwater acidification, driven by industrial runoff, agricultural activities, and atmospheric deposition, on the freshwater mollusk Bellamya bengalensis. By systematically investigating the impact of two common carboxylic acids, acetic acid (AA) and benzoic acid (BA), this research employed diverse toxicological, pathological, and ecological assessments. We explored survival predictions through the generic unified threshold model of survival (GUTS-SD), examined oxidative stress responses, and investigated hepatopancreatic alterations. In the experimental design, Bellamya bengalensis were subjected to environmentally relevant sublethal concentrations (10%, 20% LC50) of AA (39.77 and 79.54 mg/l) and BA (31.41 and 62.82 mg/l) over 28 days. Acute toxicity tests revealed increased LC50 values, indicating heightened toxicity with prolonged exposure, particularly due to the greater potency of benzoic acid compared to acetic acid. The GUTS-SD model provided accurate predictions of time-specific effects on populations, presenting long-term exposure (100 days) LC50 values for AA (263.7 mg/l) and BA (330.9 mg/l). Sequentially, the integrated biomarker response (IBR) analysis across study intervals highlighted the 28-day interval as the most sensitive, with GST emerging as the most responsive enzyme to oxidative stress induced by AA and BA. Histopathological and ultrastructural assessments of the hepatopancreas showed severe alterations, including necrosis, vacuolation and disrupted micro-villi, which were especially pronounced in higher BA exposure concentrations. These findings highlight the health and survival impacts of carboxylic acid toxicity on Bellamya bengalensis, emphasizing the need for proactive measures to mitigate acidification in aquatic ecosystems. The broader ecological implications underscore the importance of effective management and conservation strategies to address ongoing environmental challenges [Display omitted] • Investigated AA and BA effects on Bellamya bengalensis. • Used GUTS-SD model for survival and LC50 prediction. • Examined hepatopancreas for histopathology and ultrastructure. • Assessed antioxidant enzymes and lipid peroxidation. • Identified distinct AA and BA toxicity mechanisms. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the effects of propiconazole on the tubificid segmented worm, Tubifex tubifex. The animals were exposed to various concentrations of propiconazole for 96 h to assess the acute effect of this fungicide and for subacute level animals were exposed for 14 days with 10% and 20% of the 96 h LC 50 value (0.211 and 0.422 mg/l, respectively). The 96 h LC 50 value was determined to be 2.110 mg/l, and sublethal propiconazole concentrations caused significant changes in the oxidative stress enzymes. When compared to control organisms, superoxide dismutase (SOD) and catalase (CAT) activity first decreases and then significantly increases on days 7 and 14. However, GST activity decreases and MDA concentration rises in a concentration- and time-dependent manner throughout the exposure period. In addition, the impacts of propiconazole on Tubifex tubifex were characterized and depicted using a correlation matrix and an integrated biomarker response (IBR) assessment. These findings suggest that exposure to this fungicide distorts the survivability and behavioral response in Tubifex tubifex at the acute level. In addition, it modulates changes in oxidative stress enzymes at the sublethal level. Furthermore, the species sensitivity distribution curve indicates that this tubificid worm has a high risk of survival in the presence of the fungicide propiconazole in aquatic ecosystems. [Display omitted] • The toxicity of the Fungicide Propiconazole in non-lethal levels was investigated in Tubifex tubifex. • Changes in oxidative stress and histopathological alterations are found under propiconazole exposure. • Sublethal Propiconazole exposure can impact physiology of annelids in the wild. [ABSTRACT FROM AUTHOR]