1. Quantification of oxidative stress markers in the blood sera following subacute administration of different oximes in rats.
- Author
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Jaćević V, Grujić-Milanović J, Milovanović Z, Nežić L, Amidžić L, Vojinović N, Marković B, Dobričić V, Milosavljević P, Nepovimova E, and Kuča K
- Subjects
- Animals, Rats, Male, Superoxide Dismutase metabolism, Superoxide Dismutase blood, Lipid Peroxidation drug effects, Catalase metabolism, Catalase blood, Malondialdehyde blood, Malondialdehyde metabolism, Cholinesterase Reactivators pharmacology, Advanced Oxidation Protein Products blood, Antioxidants metabolism, Antioxidants pharmacology, Oxidative Stress drug effects, Oximes pharmacology, Rats, Wistar, Biomarkers blood, Glutathione blood, Glutathione metabolism
- Abstract
Oxidative stress status, as a disruption of redox homeostasis, in the blood sera of Wistar rats caused by repeated application of selected acetylcholinesterase reactivators - asoxime, obidoxime, K027, K048, K074, and K075 were evaluated. Throughout this study, each oxime in a dose of 0.1 of LD
50 /kg im was given 2x/week for 4 weeks. Then, seven days after the last oximes' application, markers of lipid peroxidation (malondialdehyde, MDA), and protein oxidation (advanced oxidation protein products, AOPP), as well as the activity of antioxidant enzymes (catalase, CAT, superoxide dismutase, SOD, reduced glutathione, GSH, and oxidized glutathione, GSSG), were determined. Oxidative stress parameters, MDA and AOPP were significantly highest in the K048-, K074- and K075-treated groups (p < 0.001). The activity of CAT was significantly elevated in the obidoxime-treated group (p < 0.05), while treatment with K027, K048, and K074 induced high elevation in SOD levels (p < 0.01, p < 0.001). Interestingly, the activity of GSH in each oxime-treated group was significantly elevated. Unlike, treatment with obidoxime caused elevation in GSSG levels (p < 0.01). As a continuation of our previously published data, these results assure that applied oximes following subacute treatment ameliorated the oxidative status and further adverse systemic toxic effects in rats., Competing Interests: Declaration of competing interest The authors declare that they have no known competing for financial interests or personal relationships that could have appeared to influence the work reported in this paper. None., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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