1. Polyphenolic profiling of Ipomoea carnea Jacq. by HPLC-DAD and its implications in oxidative stress and cancer.
- Author
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Khan MZI, Zahra SS, Ahmed M, Fatima H, Mirza B, Haq IU, and Khan SU
- Subjects
- Animals, Antioxidants analysis, Artemia drug effects, Cell Line, Tumor, Chromatography, High Pressure Liquid, Drug Screening Assays, Antitumor, Flavonoids analysis, Humans, Plant Extracts analysis, Plant Extracts pharmacology, Plants, Medicinal chemistry, Protein Kinase Inhibitors chemistry, Protein Kinase Inhibitors pharmacology, Antineoplastic Agents, Phytogenic pharmacology, Antioxidants pharmacology, Ipomoea chemistry, Oxidative Stress drug effects, Polyphenols analysis
- Abstract
Ipomoea carnea Jacq . is an important folklore medicinal plant, assessed for its underexplored biological potential. Antioxidant, cytotoxic, antiproliferative and polyphenolic profile of whole plant was evaluated using various techniques. Maximum extract recovery (29% w/w), phenolic [13.54 ± 0.27 μg GAE/mg dry weight (DW)] and flavonoid (2.11 ± 0.10 μg QE /mg DW) content were recorded in methanol-distilled water (1:1) flower extract. HPLC-DAD analysis quantified substantial amount of six different polyphenols ranging from 0.081 to 37.95 μg/mg extract. Maximum total antioxidant and reducing potential were documented in methanol-distilled water and acetone-distilled water flower extracts (42.62 ± 0.47 and 24.38 ± 0.39 μg AAE/mg DW) respectively. Ethanol-chloroform root extract manifested highest free radical scavenging (IC
50 of 61.22 μg/mL) while 94.64% of the extracts showed cytotoxicity against brine shrimps. Ethanol leaf extract exhibited remarkable activity against THP-1 cell line (IC50 = 8 ± 0.05 μg/mL) and protein kinases (31 mm phenotype bald zone).- Published
- 2019
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