1. Antioxidative, anti-inflammatory and hepatoprotective effects of resveratrol on oxidative stress-induced liver damage in tilapia (Oreochromis niloticus).
- Author
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Jia R, Li Y, Cao L, Du J, Zheng T, Qian H, Gu Z, Jeney G, Xu P, and Yin G
- Subjects
- Animals, Anti-Inflammatory Agents, Non-Steroidal administration & dosage, Antioxidants administration & dosage, Aquaculture, Biomarkers blood, Biomarkers metabolism, Cytokines agonists, Cytokines antagonists & inhibitors, Cytokines genetics, Cytokines metabolism, Dose-Response Relationship, Drug, Fish Proteins agonists, Fish Proteins antagonists & inhibitors, Fish Proteins genetics, Fish Proteins metabolism, Gene Expression Regulation, Developmental drug effects, Hydrogen Peroxide toxicity, Lipid Peroxidation drug effects, Liver cytology, Liver immunology, Liver metabolism, NF-E2-Related Factor 2 agonists, NF-E2-Related Factor 2 metabolism, Protective Agents administration & dosage, Random Allocation, Resveratrol administration & dosage, Signal Transduction drug effects, Tilapia growth & development, Toll-Like Receptor 2 agonists, Toll-Like Receptor 2 metabolism, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Antioxidants pharmacology, Liver drug effects, Oxidative Stress drug effects, Protective Agents pharmacology, Resveratrol pharmacology, Tilapia physiology
- Abstract
Resveratrol, a dietary polyphenol, has been shown to exert antioxidation, hepatoprotection, anti-inflammation and immunostimulation. However, the effects and underlying mechanism of resveratrol on liver injury in fish are still unclear. In the present study, we investigated the potential protective effects and mechanism of resveratrol on oxidative stress-induced liver damage in tilapia. Fish were fed diet containing four doses of resveratrol (0, 0.1, 0.3, and 0.6 g/kg diet) for 60 days, and then given an intraperitoneal injection of H
2 O2 or saline. The results showed that administration of resveratrol significantly ameliorated H2 O2 -induced liver injury. In serum and liver, resveratrol treatment suppressed the oxidative stress, as evidenced by the decline of lipid peroxidation level and increase of antioxidant activity. Resveratrol also activated erythroid 2-related factor 2 (Nrf2) signaling pathway and enhanced the heme oxygenase 1 (HO-1), NAD(P) H:quinone oxidoreductase 1 (NQO-1), glutathione S-transferase (GST) mRNA levels. Meanwhile, resveratrol treatment repressed TLR2-Myd88-NF-κB signaling pathway to decrease the inflammatory response in H2 O2 -induced liver injury as evidenced by the lower interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and IL-8 mRNA levels and higher IL-10 mRNA level. Moreover, resveratrol treatment attenuated immunotoxicity in liver of H2 O2 -treated fish, accompanied by upregulation of hepcidin (HEP), complement 3 (C3) and lysozyme (LZM) mRNA levels. Overall results suggested that the protection of resveratrol on H2 O2 -induced liver injury, inflammation and immunotoxicity was due to its antioxidant property and its ability to modulate the Nrf2 and TLR2-Myd88-NF-κB signaling pathways., (Copyright © 2018 Elsevier Inc. All rights reserved.) more...- Published
- 2019
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