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Start Over Descriptor "anhui white goat" Topic ovary
2 results on '"anhui white goat"'

1. Characterization and differential expression of microRNAs in the ovaries of pregnant and non-pregnant goats (Capra hircus).

Author

Zhang, Xiao-Dong, Zhang, Yun-Hai, Ling, Ying-Hui, Ya Liu, Cao, Hong-Guo, Yin, Zong-Jun, Ding, Jian-Ping, and Zhang, Xiao-Rong

Subjects
*MAMMAL reproduction, *GOATS, *PREGNANCY in animals, *GENE expression, *MICRORNA, *NUCLEOTIDE sequence, *OVARIAN follicle, *TRANSCRIPTION factors, *GENETIC regulation, *SECRETION
Abstract

Background: Ovarian follicular development and hormone secretion are complex and coordinated biological processes which will usually be altered during pregnancy. Ovarian function is tightly regulated by a multitude of genes, and also by some specific miRNAs. It is necessary to identify the differentially expressed miRNAs in the ovaries of pregnant and non-pregnant mammals, in order to further understand the role of miRNA-mediated posttranscriptional regulation in mammalian reproduction. Here, we performed a comprehensive search for hircine miRNAs using two small RNA sequencing libraries prepared from the ovaries of pregnant and non-pregnant goats. Results: 617 conserved and 7 putative novel miRNAs were identified in the hircine ovaries. A total of 471 conserved miRNAs (76.34%) were co-expressed in both pregnant and non-pregnant libraries, and 90 pregnancyspecific and 56 non-pregnancy-specific conserved miRNAs were identified. Additionally, 407 unique miRNAs (65.96%) were significantly differentially expressed in the pregnant and non-pregnant libraries, of which 294 were upregulated and 113 were downregulated in the pregnant library compared to the non-pregnant library. Further analysis showed that miR-143 was predicted to bind to the target sequences of Frizzled-6 and -3 receptor genes in the Wnt/beta-catenin signaling pathway, and let-7b may target the Activin receptor I and Smad 2/3 genes in the TGF-beta signaling pathway. The expression level of 5 randomly selected miRNAs were analyzed by quantitative real-time PCR (q-PCR), and the results demonstrated that the expression patterns were consistent with the Solexa sequencing results. Conclusions: The identification and characterization of differentially expressed miRNAs in the ovaries of pregnant and non-pregnant goats provides important information on the role of miRNA in the regulation of the ovarian development and function. This data will be helpful to facilitate studies on the regulation of miRNAs during mammalian reproduction. [ABSTRACT FROM AUTHOR]

Published
2013
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2. Characterization and differential expression of microRNAs in the ovaries of pregnant and non-pregnant goats (Capra hircus)

Author

Hongguo Cao, Yinghui Ling, Xiao-Dong Zhang, Xiaorong Zhang, Jianping Ding, Ya Liu, Zongjun Yin, and Yunhai Zhang

Subjects
Small RNA, Solexa sequencing, Ovary, Breeding, Biology, Conserved sequence, Pregnancy, microRNA, Genetics, medicine, Animals, Gene, Conserved Sequence, reproductive and urinary physiology, Base Sequence, Gene Expression Profiling, Goats, Reproduction, Wnt signaling pathway, High-Throughput Nucleotide Sequencing, Reproducibility of Results, MicroRNA, Anhui white goat, Gene expression profiling, MicroRNAs, medicine.anatomical_structure, Female, DNA microarray, Research Article, Biotechnology
Abstract

Background Ovarian follicular development and hormone secretion are complex and coordinated biological processes which will usually be altered during pregnancy. Ovarian function is tightly regulated by a multitude of genes, and also by some specific miRNAs. It is necessary to identify the differentially expressed miRNAs in the ovaries of pregnant and non-pregnant mammals, in order to further understand the role of miRNA-mediated post-transcriptional regulation in mammalian reproduction. Here, we performed a comprehensive search for hircine miRNAs using two small RNA sequencing libraries prepared from the ovaries of pregnant and non-pregnant goats. Results 617 conserved and 7 putative novel miRNAs were identified in the hircine ovaries. A total of 471 conserved miRNAs (76.34%) were co-expressed in both pregnant and non-pregnant libraries, and 90 pregnancy-specific and 56 non-pregnancy-specific conserved miRNAs were identified. Additionally, 407 unique miRNAs (65.96%) were significantly differentially expressed in the pregnant and non-pregnant libraries, of which 294 were upregulated and 113 were downregulated in the pregnant library compared to the non-pregnant library. Further analysis showed that miR-143 was predicted to bind to the target sequences of Frizzled-6 and -3 receptor genes in the Wnt/beta-catenin signaling pathway, and let-7b may target the Activin receptor I and Smad 2/3 genes in the TGF-beta signaling pathway. The expression level of 5 randomly selected miRNAs were analyzed by quantitative real-time PCR (q-PCR), and the results demonstrated that the expression patterns were consistent with the Solexa sequencing results. Conclusions The identification and characterization of differentially expressed miRNAs in the ovaries of pregnant and non-pregnant goats provides important information on the role of miRNA in the regulation of the ovarian development and function. This data will be helpful to facilitate studies on the regulation of miRNAs during mammalian reproduction.

Published
2013

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