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Alpha and beta-hCG subunits were recombined generating a hybrid hCG preparation (AB1ER-CR-2XY) which met the required specifications of a pharmaceutical product. The biologic activity contained in each vial of AB1ER-CR-2XY was equivalent to 10 000IU of hCG-IS. This preparation was given as a single dose of 10 000IU by the i.m. route to four female subjects presenting unexplained infertility. The hCG hybrid was demonstrated to effect gonadal stimulation in humans.

Tannates of ovine pituitary follicle stimulating hormone (NIH-FSH-S1 and NIH-FSH-S2) were prepared and assayed simultaneously with equal amounts of untannated FSH. Three assay systems were employed-a general gonadotropin assay utilizing both ovarian and uterine weights of intact, immature female rats, the standard augmentation reaction assay, and modifications of both assays conducted at different time intervals with either single or multiple injection schedules. FSH activity as determined by standard or modified assay systems was not potentiated by tannation. (Endocrinology 79: 149, 1966)

Tannate complexes of human pituitary gonadotropin (HPG) of urinary origin were assayed by 3 general gonadotropin assay methods-the rat ovarian and uterine weight methods and the mouse uterine weight method. Assays were also performed by injecting single doses · of tannated and untannated HPG and determining the magnitude of the response at daily intervals. It was found that tannation did not alter the potency of HPG as measured in these 3 assay systems. The results obtained with single-dose assays confirmed those obtained by conventional assays. (Endocrinology 77: 766,1965