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SUMMARY: Few studies have modelled the nutrient requirements of breeding ostriches. Results obtained from historical studies were used here to determine the energy, protein and amino acid requirements of breeding ostriches for maintenance and egg production. Two energy scales were used to determine the energy requirement for egg production by ostrich breeders. The efficiency of metabolisable energy (ME) utilisation for energy deposition in the egg (ko) was calculated as 0.8, and the ME required for egg production (MEe) was 12.2 MJ/egg. The effective energy (EE) requirement for egg production (EEe) and maintenance (EEm) was calculated as 7.8 and 13.8 MJ/bird d, respectively. Average total daily balanced protein requirement (TPt) was calculated as 160 g/bird d. Amino acid requirements for maintenance and egg production are given together with recommendations for the daily supply of each amino acid. [ABSTRACT FROM AUTHOR]

Vocal production in birds has been the target of considerable research that mostly has focused on phylogenetically well‐nested songbirds. Anatomical descriptions and recordings of many non‐songbirds have often only focused on a single ontogenetic stage or sex. While basic morphology of the vocal organ (syrinx) of ostrich (Palaeognathae, Struthio camelus) has been known since the 1800s, descriptions of its vocal repertoire and syrinx anatomy since then have been incomplete or inconsistent. New toolkits now enable detailed qualitative description of internal anatomy and meristic data and allow it to be compared to vocal production. Here we describe the anatomy of the syrinx in Struthio camelus for three post‐hatching ontogenetic stages and both an adult male and female utilizing dissection and contrast enhanced X‐ray computed tomography (diceCT). We find changes in ring geometry and spacing through ontogeny as well as lateral labia thickness. We document a small unpaired, midline, cartilaginous structure, a "pessuliform process" at the tracheobronchial juncture present throughout ontogeny and in both males and females. Investigation of the vocal repertoire of ostriches across ontogeny using a new dataset of 77 recordings led to identification of four vocalizations not previously reported in the literature, including the simultaneous production of a hiss and tonal. We find syrinx morphology largely consistent across ontogeny and in male and female adults. Both are capable of producing long duration tonal calls, but these may be more frequent in male birds. Closed‐mouth boom calls remain unique to males. A detailed understanding of diversity in parts of early diverging clades is pivotal in attempting to estimate features of the ancestral syrinx in birds and how avian vocalization evolved. [ABSTRACT FROM AUTHOR]

Ostrich meat has a very high nutritional value, and improving the shelf life of raw ground meat is highest importance for the meat industry. The present study aims to improve the quality of minced ostrich meat using biosurfactants produced by Lactobacillus strains. For this purpose, biosurfactants were first derived from probiotic bacteria including Lactiplantibacillus plantarum, Lactobacillus pentosus, and Lactobacillus casei. Then, the antioxidant activity of biosurfactant was evaluated in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl free radicals, and antimicrobial activities studied using the diffusion method in agar wells against pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Next, biosurfactants (with a concentration of 0.05 g/ml) were evaluated as biological preservatives to improve the microbiological (TVC, PTC, E. coli, S. aureus, and mold and yeast) and physicochemical (PV, TBA, TVB-N, pH, hardness and color index) quality of minced ostrich meat during 16 days of storage at 4 °C. The data analysis was performed using SPSS statistical software. The results showed that the scavenging activity of DPPH and hydroxyl of biosurfactants at a concentration of 0.05 g/ml was 81.99–78.51% and 71.56–69.56%, respectively, and all biosurfactants had high antimicrobial activities. The results related to the shelf life of ostrich showed that the use of biosurfactants resulted in better color stability and firmer texture of the samples during the storage period. It was also the cause of delaying the chemical and microbial spoilage of the samples during the storage period. The samples containing biosurfactants had chemical, microbial, and sensory limits until the end of the storage period. Based on the results, biosurfactants can reduce all the indicators mentioned as natural antioxidants and antimicrobials, thus increasing the she lf life of minced ostrich meat. In addition, they can provide the necessary background for the practical use of these compounds in all kinds of meats and their products. [ABSTRACT FROM AUTHOR]

Copyright of Sport & Tourism Central European Journal / Sport i Turystyka Srodkowoeuropejskie Czasopismo Naukowe is the property of Jan Dlugosz University in Czestochowa and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In Egypt, ostrich farming has had a great increase during the last few years. The most common problems facing ostrich farmers are a high percentage of late embryonic mortality and low hatchability. This study was designed to survey the main causes of late in-shell ostrich embryonic death. A total of 100 freshly dead inshell ostrich embryos, all of them were in the second half of the incubation period with different ages ranging from 25 to 39 days old, were received during the breeding seasons of the period from October 2018- October 2021, from different ostrich farms in Egypt, suffered from late-stage embryonic death during artificial incubation, respiratory signs and diarrhea in breeders, moreover, the surveyed ostrich farms were near to chicken farms suffered from Newcastle Disease outbreaks. Gross findings in all embryos were recorded as anasarca, severe general congestion, and hemorrhages. In this study, 66 dead shell ostrich embryos out of 100 (66%) were immunohistochemically positive for Newcastle Disease Virus. Four Velogenic Newcastle Disease Virus strains (genotype VIIb) were molecularly characterized in 4 immune-positive ND embryos (from 4 different ostrich farms in 4 different Egyptian governorates) by RT-PCR and F gene sequencing. In addition, all embryos were negative for bacterial isolation except for one vNDV positive embryo (1%) (25 days old) that was superinfected by Citrobacter spp. The main histopathological lesions of vNDV-positive ostrich embryos were general edema, extensive congested blood vessels, hemorrhages, necrosis, syncytial cells formation in the upper respiratory tract, and leukocytic cells infiltrations. To the best of our knowledge, this study is the first report of the Velogenic Newcastle Disease Virus that immunohistochemically and molecularly characterized in dead ostrich embryos in Egypt, therefore, Newcastle Disease Virus vaccination of ostrich breeders is strongly recommended. [ABSTRACT FROM AUTHOR]

Objective: To analyze 5 types of poultry eggs (chicken, turkey, ostrich, duck and quail) to compare their nutritional characteristics. Design/Methodology/Approach: A physical analysis was performed: weight of the entire egg (weight and proportion of the albumin, yolk and shell) length and width of the entire egg, shape index, shell color, and yolk color, nutritional (determination of raw fat, protein, dry matter and ashes). Different types of egg used: chicken, turkey, ostrich, duck and quail. Results: The egg containing the most amount of protein was that of the duck (13.02 ± 0.46%), while the sample containing the lowest result was that of the ostrich (9.47 ± 0.27%). The type of egg that contained the fattest level was the duck (10.31 ± 0.75%); on the other hand, the type of egg that demonstrated the least amount of fat was that of the chicken egg (8.28 ± 0.39%). Results/Findings/Conclusion: Even though some physical differences exist in all types of eggs, they are similar and there is minimal variation in terms of their nutritional value. Therefore, these different types of eggs can be applied for consumption as substitutes for chicken eggs and as an alternative source of protein. Limitations of the study/Implications: Lack of previous research in regard to comparisons of the types of analyzed eggs. [ABSTRACT FROM AUTHOR]

Copyright of MESO is the property of Zadruzna Stampa D.D. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Few studies describe the sequence of morphological events that characterize spermiogenesis in birds. In this paper, the clearly observable steps of spermiogenesis are described and illustrated for the first time in a commercially important ratite, the ostrich, based on light microscopy of toluidine blue-stained plastic sections. Findings were supplemented and supported by ultrastructural observations, PNA labeling of acrosome development, and immunocytochemical labeling of isolated spermatogenic cells. Spermiogenesis in the ostrich followed the general pattern described in non-passerine birds. Eight steps were identified based on changes in nuclear shape and contents, positioning of the centriolar complex, and acrosome development. Only two steps could be recognized with certainty during development of the round spermatid which contributed to the fewer steps recorded for the ostrich compared to that described in some other bird species. The only lectin that displayed acrosome reactivity was PNA and only for the first three steps of spermiogenesis. This suggests that organizational and/or compositional changes may occur in the acrosome during development and merits further investigation. Immunological labeling provided additional evidence to support the finding of previous studies that the tip of the nucleus in the ostrich is shaped by the forming acrosome and not by the microtubular manchette. To our knowledge, this is the first complete description of spermiogenesis in ostrich and one of few in any avian species. In addition to comparative reproduction and animal science, this work has implications for evolutionary biology as the reported germ cell features provide a bridge between reptile and ratite-avian spermatogenesis. [ABSTRACT FROM AUTHOR]

Examinations on ostrich chick vitality can help to improve the effectiveness of Hungarian ostrich husbandry and hatching technology. The investigations were carried out on an ostrich breeder farm in Eastern Hungary. For the analyses, the Tona et al. (2003) scoring system was applied presenting eight main criteria (activity, feather, condition, eyesight, the condition of navel and leg, the amount of the remaining shell membrane and egg content, and the size and tenderness of the abdomen). Most chicks (98.08%) were vigilant and had clean and dry feathers. There was no difference between the average hatching weights between May and August but a decreasing tendency was shown during the laying season. The vitality total score was above 90 in each evaluated month and was not affected by the hatching month. Our results revealed that the vitality of chicks was not affected by either the hatching month (P=0.51) or the weight category (P=0.11). Neither the hatching weight of chicks and leg condition were correlated (P=0.79). Results showed that the Tona scoring system with small modifications can be well applied to ostrich chicks. Practical on-farm usage of the system can be suggested as an aid in day-old chick evaluation. After individual marking of chicks, the investigation of growth and culling rate could be carried out for more precise conclusions, involving more farms and laying seasons. [ABSTRACT FROM AUTHOR]

Background: The chicken has been a representative model organism to study embryonic development in birds, however important differences exist among this class of species. As a representative of one of oldest existing clades of birds, the African ostrich (Struthio camelus), has the largest body among birds, and has two toes. Our purpose is to establish the corresponding stages in ostrich embryo development that match the well‐established HH system of the chicken to facilitate comparative studies between the ostrich and other birds to better understand differences in development. Results: Here we describe in detail the middle period of embryonic development using microscopic images and skeletal staining. We found that clear morphological differentiation between the ostrich and the chicken begins at stage 26. Bird limb cartilage first form in stage 25, while the development of the limb skeletons differs after stage 31. Calcification of limb skeletons in the chicken was completed faster. The first and second toes of the ostrich disappear at stages 36 and 38, respectively. Conclusions: This study should greatly aid ostrich‐related developmental and morphological research and provide a reference for studying the development and evolution of avian limb skeletons, including molecular research. Questions that can now be addressed include studies into the fusion of tarsometatarsal skeleton, ossification, and digit loss. Key Findings: The stages of ostrich development are defined.Comparison of embryonic development between ostrich and chicken.Continuously record and describe limb bone development in the ostrich and chicken.Limb cartilage first forms in both species at stage 25.The unique degenerated first and second toes of the ostrich is completed by stages 36 and 38, respectively. [ABSTRACT FROM AUTHOR]

Ostriches are known to be the fastest bipedal animal alive; to accomplish such an achievement, their anatomy evolved to sustain the stresses imposed by running at such velocities. Ostriches represent an excellent case study due to the fact that their locomotor kinematics have been extensively studied for their running capabilities. The shape and structure of ostrich bones are also known to be optimized to sustain the stresses imposed by the body mass and accelerations to which the bones are subjected during movements. This study focuses on the limb bones, investigating the structure of the bones as well as the material properties, and how both the structure and material evolved to maximise the performance while minimising the stresses applied to the bones themselves. The femoral shaft is hollowed and it presents an imbricate structure of fused bone ridges connected to the walls of the marrow cavity, while the tibial shaft is subdivided into regions having different mechanical characteristics. These adaptations indicate the optimization of both the structure and the material to bear the stresses. The regionalization of the material highlighted by the mechanical tests represents the capability of the bone to adapt to external stimuli during the life of an individual, optimizing not only the structure of the bone but the material itself. [ABSTRACT FROM AUTHOR]

Copyright of Journal of the Chinese Society of Animal Science is the property of Chinese Society of Animal Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

By applying a sinusoidal function (as a trigonometric model), this study aimed to introduce this function into ostrich weight development research, using ostrich growth data from the literature and comparing it with some routinely used growth models such as monomolecular, Bridges, Janoschek, logistic, Von Bertalanffy, Richards, Schumacher, Morgan, Chanter, and Weibull. During the fitting of nonlinear regression curves, model performance was evaluated and model behavior was examined. Body weight data of the domestic ostriches used in this study were reported in the Blue Mountain Ostrich Nutrition e-bulletin from three different studies (data sets 1 to 3). In all data sets, body weight was measured monthly from one to twelve months of age. The adjusted coefficient of determination, root mean square error, Akaike's information criterion, and Bayesian information criterion were used to evaluate each model's overall goodness-of-fit to different data profiles. Based on the goodness-of-fit criteria, the sinusoidal model was determined to be the most suitable function for fitting the growth curve of ostriches in data sets 1 and 2. However, both monomolecular and logistic models had the worst fit to the growth curve of ostriches in these data sets. For data set 3, the Weibull model provided the best fit of the growth curve of ostriches, but the sinusoidal function had the worst fit. Absolute growth rate (AGR), calculated using the first derivative of the best model with time showed that AGR values ​​increased with age until days 174, 90, and 68 for data sets 1 to 3, respectively, and then decreased. Overall, this study offers implications for advancing research on ostrich production systems and providing insightful information on the application of alternative nonlinear models in modeling ostrich growth. [ABSTRACT FROM AUTHOR]

A complete ostrich femur from the Late Pleistocene deposits of the Upper Cave at Zhoukoudian (China) was referred by Shaw to Struthio anderssoni in the 1930s, but its present whereabouts are unknown. A good quality plaster cast of the missing specimen has been found in the collections of the Natural History Museum (London). This cast provides interesting information about the morphology of this large ostrich femur, which had previously been only summarily described and not illustrated. Although smaller than the femora of the Early Pleistocene giant ostrich Pachystruthio, the robust femur from Zhoukoudian shows morphological similarities with them, and it is suggested that 'Struthio' anderssoni should be placed in the genus Pachystruthio. The importance of old palaeontological casts is emphasized, as well as the need to preserve and curate them properly. [ABSTRACT FROM AUTHOR]

Background: Evaluating the prevalence of vancomycin resistance genes (van genes) in enterococcal isolates from food‐producing animals is an important public health issue because of the possibility of resistance genes spread to human. Objectives: The present study aimed to determine the occurrence of vancomycin resistance genes among Enterococcus species obtained from ostrich faecal samples. Methods: One hundred and twenty‐five faecal samples of apparently healthy ostriches from five different farms were investigated. Genes encoding vancomycin resistance were studied by multiplex‐PCR, and susceptibility to six antibiotics was evaluated by disk‐diffusion method. Results: In total, 107 Enterococcus spp. isolates were obtained and confirmed by biochemical and molecular tests. Enterococcus faecium was the prevailing species (56 isolates of 107; 52.3%), followed by E. hirae (24 isolates; 22.4%) and E. gallinarum (12 isolates; 11.2%). Of the 107 recovered isolates, 44% harboured at least a type of van genes. vanA, vanC2/3 and vanC1 were identified in 34 (31.7%), 13 isolates (12.1%) and 4 (3.7%) isolates respectively. Additionally, four isolates (E. gallinarum, E. rafinosus) co‐harboured the the vanA and vanC1 or vanA and vanC2/3. Enterococcus faecium and Enterococcus hirae strains with the vanA genotype were the most frequent van‐carrying enterococci from ostrich faecal samples. Among van‐carrying enterococcal isolates, 23.4% were phenotypically resistant to vancomycin. This study revealed a relatively high prevalence (44%) of van‐carrying enterococci in ostrich faecal samples. Conclusions: Results of the present study suggest that ostrich faeces could be considered as a reservoir of vancomycin resistance genes, especially vanA containing enterococci that could be potentially transferred to human through the food chain. [ABSTRACT FROM AUTHOR]

Field surveys over the past decade in the Sperrgebiet, Namibia, have led to the recovery of additional fossil remains of the Early Miocene ostrich, Struthio coppensi Mourer-Chauviré et al. 1996, including three fossils from a locality, Grillental VI, from which the species had not previously been recorded. Among the new material, there are two pedal phalanges and a cervical vertebra, as well as additional leg bones (tibiotarsus, tarsometatarsus). Descriptions and illustrations are provided of the material, which conform in dimensions and morphology with what would be expected for Struthio coppensi, which had ca 65% the dimensions of the extant ostrich, Struthio camelus. [ABSTRACT FROM AUTHOR]

To look for the reason why the biped animal in nature can run with such high speed and to design a bionic biped prototype which can behave the high speed running and jumping ability, this paper takes the fastest bipedal animal in nature: ostrich as the research subject. Firstly, the body structure and motion characteristics of ostrich are investigated. Secondly, a simple mechanical structure of bionic ostrich robot is designed based on the above biological investigated results. The robot is under-actuated with one actuator each leg, with a spring on the tarsometatarsus and a torsion spring on the metatarsophalangeal joint at the foot end. And then the mechanical design of leg structure is optimized. Finally, the high-speed running and jumping running gait is planned, and comparative simulations are implemented with different design requirements among pure rigid and rigid-flexible coupling scheme, which are rigid, only with spring, only with torsion spring, and with spring and torsion spring both, in detail. Simulation results show that the rigid-flexible coupling design scheme and whole body motion coordination can achieve better high speed performance. It provides an insight for the design and control of legged robots. [ABSTRACT FROM AUTHOR]

Recent studies have revealed the dynamic and complex evolution of CLCA1 gene homologues in and between mammals and birds with a particularly high diversity in mammals. In contrast, CLCA2 has only been found as a single copy gene in mammals, to date. Furthermore, CLCA2 has only been investigated in few mammalian species but not in birds. Here, we established core genomic, protein biochemical and expressional properties of CLCA2 in several bird species and compared them with mammalian CLCA2. Chicken, turkey, quail and ostrich CLCA2 were compared to their mammalian orthologues using in silico, biochemical and expressional analyses. CLCA2 was found highly conserved not only at the level of genomic and exon architecture but also in terms of the canonical CLCA2 protein domain organization. The putatively prototypical galline CLCA2 (gCLCA2) was cloned and immunoblotting as well as immunofluorescence analyses of heterologously expressed gCLCA2 revealed protein cleavage, glycosylation patterns and anchoring in the plasma membrane similar to those of most mammalian CLCA2 orthologues. Immunohistochemistry found highly conserved CLCA2 expression in epidermal keratinocytes in all birds and mammals investigated. Our results suggest a highly conserved and likely evolutionarily indispensable role of CLCA2 in keratinocyte function. Its high degree of conservation on the genomic, biochemical and expressional levels stands in contrast to the dynamic structural complexities and proposed functional diversifications between mammalian and avian CLCA1 homologues, insinuating a significant degree of negative selection of CLCA2 orthologues among birds and mammals. Finally, and again in contrast to CLCA1, the high conservation of CLCA2 makes it a strong candidate for studying basic properties of the functionally still widely unresolved CLCA gene family. [ABSTRACT FROM AUTHOR]

The first fossil ostrich to have been named, by Milne-Edwards in 1869–1871, was Struthio asiaticus, a taxon based on a specimen collected by Colonel Colvin in the Siwaliks of India, consisting of associated postcranial elements (vertebrae, forelimb elements, a fragmentary tarsometatarsus and first phalanx of the third toe). Although it was described as least twice in some detail in the 19th century, the specimen has since then been interpreted in conflicting ways. A revision of the type material and its history shows that it came in all likelihood from the Siwaliks of present-day India, not Pakistan. The exact locality is unknown and the stratigraphic position of the specimen is uncertain (the frequent attribution to the Dhok Pathan Formation is not based on solid evidence). Contrary to what has sometimes been claimed, Struthio asiaticus was neither a small nor an especially large ostrich. It was apparently comparable in size with the modern S. camelus, but had slightly more robust cervical vertebrae and carpal bones. It seems to have been relatively short-toed. Because of the incompleteness of the material and uncertainties about its geological age, it is difficult to assess the place of Struthio asiaticus in the evolutionary history of the ostriches. Reports of Struthio asiaticus from Africa and north-eastern Asia are based on misinterpretations and should be discarded. [ABSTRACT FROM AUTHOR]

The study aimed to investigate the effect of the combination of year and season of breeding period, egg weight, the annual hatching order of chicks and the hatchability of eggs on the relative risk of total loss for chicks during their 48-week life period. The examination was conducted on one of the largest farms in Hungary working with sixty breeding birds, kept in trios (one male and two females). The research covered the growing information of 1606 chicks hatched in 2019, 2020 and 2021. The highest relative risk of total loss was revealed in autumn 2020 and 2021. In comparison with the reference group (2021 summer), the relative risk of total loss was significantly lower in summer 2019 (p < 0.05) and spring 2020 (p = 0.0049) 2020 (p = 0.0000) and 2021 (p = 0.0348) spring. Both between years and between seasons the tendency of relative risk was the same. The relative risk of total loss increased until the end of the third quart of the incubation period, then remained unchanged. Chicks from groups having weak hatchability (≤50%) had the highest relative risk (p < 0.05). [ABSTRACT FROM AUTHOR]

Ostriches and emus are among the largest extant birds and are frequently used as modern analogs for the growth dynamics of non‐avian theropod dinosaurs. These ratites quickly reach adult size in under 1 year, and as such do not typically exhibit annually deposited growth marks. Growth marks, commonly classified as annuli or lines of arrested growth (LAGs), represent reduced or halted osteogenesis, respectively, and their presence demonstrates varying degrees of developmental plasticity. Growth marks have not yet been reported from ostriches and emus, prompting authors to suggest that they have lost the plasticity required to deposit them. Here we observe the hind limb bone histology of three captive juvenile emus and one captive adult ostrich. Two of the three juvenile emus exhibit typical bone histology but the third emu, a 4.5‐month‐old juvenile, exhibits a regional arc of avascular tissue, which we interpret as a growth mark. As this mark is not present in the other two emus from the same cohort and it co‐occurs with a contralateral broken fibula, we suggest variable biomechanical load as a potential cause. The ostrich exhibits a complete ring of avascular, hypermineralized bone with sparse, flattened osteocyte lacunae. We identify this as an annulus and interpret it as slowing of growth. In the absence of other growth marks and lacking the animal's life history, the timing and cause of this ostrich's reduced growth are unclear. Even so, these findings demonstrate that both taxa retain the ancestral developmental plasticity required to temporarily slow growth. We also discuss the potential challenges of identifying growth marks using incomplete population data sets and partial cortical sampling. [ABSTRACT FROM AUTHOR]

We propose a wire-driven robotic arm inspired by the ostrich neck. It can pick up a small piece of feed from the ground while colliding with it. This arm is named robostrich arm (shortened form of robotic ostrich arm). It consists of a serial chain of 18 rigid bodies connected by free rotational joints that are designed to have angle limitations similar to the bones of a real ostrich. It moves in a vertical plane and is driven by two DC motors through antagonistic wires. The task considered in this study was to lift the arm tip (the "head" of the robostrich arm). The experimental results indicate that the tensioner balance and timing between the two wires are important for achieving the head-up task. This paper indicates the contribution of antagonist muscles to the performance of head-up tasks by high-degree-of-freedom underactuated manipulators in robotics and ostrich necks in biological studies. [ABSTRACT FROM AUTHOR]

Background: Rigorous testing is a prerequisite to prove freedom of notifiable influenza A virus infections in commercially farmed ostriches, as is the isolation and identification of circulating strains. Pooling 5 ostrich tracheal swabs in a 50 % v/v phosphate-buffered saline (PBS): glycerol transport medium (without antibiotics) is the current standard practice to increase reverse transcription real time PCR (RT-rtPCR) testing throughput and simultaneously reduce the test costs. In this study we investigated whether doubling ostrich tracheal swabs to 10 per pool would affect the sensitivity of detection of H5N8 high pathogenicity avian influenza virus (HPAIV) and H7N1 low pathogenicity avian influenza virus (LPAIV) by quantitative RT-rtPCR, and we also compared the effect of a protein-rich, brain heart infusion broth (BHI) virus transport media containing broad spectrum antimicrobials (VTM) on the efficacy of isolating the H5N8 and H7N1 viruses from ostrich tracheas, since the historical isolation success rate from these birds has been poor. Results: Increasing the ostrich swabs from 5 to 10 per pool in 3 mls of transport medium had no detrimental effect on the sensitivity of the RT-rtPCR assay in detecting H5N8 HPAIV or H7N1 LPAIV; and doubling of the swab pool size even seemed to improve the sensitivity of virus detection at levels that were statistically significant (p less than or equal to 0.05) in medium and low doses of spiked H5N8 HPAIV and at high levels of spiked H7N1 LPAIV. On virus isolation, more samples were positive when swabs were stored in a protein-rich viral transport medium supplemented with antimicrobials in PBS: glycerol (10/18 vs. 7/18 for H5N8 HPAI); although the differences were not statistically significant, overall higher virus titres were detected (106.7 – 103.0 vs. 106.6 - 103.1 EID50 for H5N8 HPAIV and 105.5 - 101.4 vs. 105.1 – 101.3 EID50 for H7N1 LPAIV); and fewer passages were required with less filtration for both H5N8 HPAI and H7N1 LPAI strains. Conclusion: Ostrich tracheal swab pool size could be increased from 5 to 10 in 3mls of VTM with no loss in sensitivity of the RT-rtPCR assay in detecting HPAI or LPAI viruses, and HPAI virus could be isolated from a greater proportion of swabs stored in VTM compared to PBS: glycerol without antibiotics. [ABSTRACT FROM AUTHOR]

Copyright of Brazilian Journal of Biology is the property of Instituto Internacional de Ecologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Traumatic ventriculitis or hardware disease is the gizzard perforation by sharp foreign bodies (metallic or sharp object) that leads to ulcerative lesions. The present Case Report was to investigate through post mortem examination the cause of African ostrich death. Necropsy was conducted on a dead male adult African Ostrich presented to the post mortem unit of the Department of Veterinary Pathology, University of Ilorin, Ilorin, Nigeria. The gross lesions showed multifocal ecchymotic and petechial haemorrhages and lacerations on the left shank and thigh as well as the cloaca and vent of the bird. A perforation of the proventriculus with adhesion of a pointed nail-like metallic sharp object of 9.4 cm in length was observed. Histopathological findings revealed obliteration of the tubules and vascular congestion which predisposed the animal to metabolic disorder. [ABSTRACT FROM AUTHOR]

Avian influenza surveillance is a requirement for commercial trade in ostrich products, but influenza A viruses (IAVs) have proven difficult to isolate from ostrich tracheal swabs that test positive using molecular methods. We hypothesized that microbes unique to the ostrich trachea propagate in the transport medium after sampling and affect viral viability. We cultured tracheal swabs from 50 ostriches on 4 farms in South Africa, and recovered and identified 13 bacterial, 1 yeast, and 2 fungal species. Dietzia sp. had not been identified previously in the oropharyngeal tract of a bird, to our knowledge. The bacteria were tested for antimicrobial susceptibility, and most aerobic species, except for Streptococcus sp. and Pseudomonas sp., were sensitive to enrofloxacin; all were susceptible to sulfonamide. Virus inhibition experiments determined that ostrich-source Streptococcus sp., Pantoea sp., and Citrobacter freundii produced extracellular metabolites that caused a substantial reduction in the IAV titers of 99.9%. Streptomyces, Corynebacterium, Staphylococcus, Arthrobacter gandavensis, Pseudomonas putida, and Acinetobacter spp. similarly reduced the viability of IAV from 77.6% to 24.1%. Dietzia appeared to have no effect, but Rothia dentocariosa, Rhodotorula spp., and Clostridium spp. slightly increased the viability of IAV by 25.9, 34.9, and 58.5%, respectively. [ABSTRACT FROM AUTHOR]

This study describes the ostrich reproduction behavior under farming conditions, observed during reproduction seasons. Behavioral observations were made in an intensive farm from Romania who implemented the trio breeding system that includes a male and two ostrich females. The main categories of identified behavior at captive ostrich were: courtship, feeding behavior, drinking, lithophagia, walking, resting and aggression. Both the male and major female participating in incubation and chick care. Presence of eggs incited ostriches to sit in the nest, because of this all the eggs were collected and hatched artificially. In early spring, complex courtship rituals begin, performed by both males and females, who spend about 30% of their time. Resting behavior is observed when ostrich stopped moving from doing any activities. The time spent at rest was about 25%. Locomotion behavior includes walking and running. Walking (20%) was the most frequent locomotion behavior observed. Regarding the feeding behavior of the breeding families, the highest share were fed with green fodder about 7% while concentrated food was 6%. During the breeding period, the male is very protective and guards his territory and nest and the aggressiveness towards the caretaker is high 4% when collecting eggs, precautions must be taken during the collection of eggs and at males kept in neighboring shelters. Understanding the ostrich reproduction behavior under farming conditions is a vital step in improving their welfare. [ABSTRACT FROM AUTHOR]

The primary goal of this study was to document the gross morphological and histological structure and histomorphometrical measurements of the intestines and digestive glands of ostrich (Struthio camelus) in relation to advancing age and sex. A total of 40 clinically healthy ostriches of either sex [(20 males, 20 females comprising of two age groups, viz, immature (1 month to 2.4 years) and adult (2.5 to 10 years)] of equal size were selected. Immediately after collection, morphological studies were carried out on all organs. Histological and histometrical studies were conducted on paraffin-embedded tissue sections with Image J® analysis software. Macroscopic parameters of the intestines, liver and pancreas invariably showed a rapid increase (P<0.05) during immature age but maintained a plateau with negligible increment in an adult age group. Contrary to other avian species, the length of the small intestine in adult ostriches was 706 cm and the large intestine was 1218 cm which means the length of the large intestine is 1.73 times greater than the small intestine. The length of the colon is also larger than the comparable avian species which increases the digestion of fiber. Thicknesses of all layers of intestines and various parameters of digestive glands had a significant (P<0.05) increase in adults compared to immature ostriches. The growth and maturation of digestive organs in ostriches were not related to sex in each age group. These findings can be extremely beneficial for strategic manipulation of feed and nutrition to enhance the growth rate and diagnose pathological processes. [ABSTRACT FROM AUTHOR]

The aim of the study was to evaluate lipid and protein oxidation during refrigerated storage (0, 4, 8, 12 and 16 days) and in vitro gastrointestinal digestion of ostrich meat (M. iliofibularis), which was packed either under vacuum (VP) or modified atmosphere (MAP) in two combination of gases O2:CO2:N2, being 40:40:20 (MAP 1) and 60:30:10 (MAP 2). Lipid oxidation was assessed by malondialdehyde (MDA), 4-hydroxy-2-nonenal (4-HNE), and hexanal (HEX), and protein oxidation was evaluated by the formation of protein carbonyl content compounds (PCC). In raw ostrich meat packed under both MAP conditions, an increase of MDA and 4-HNE levels was observed during storage, while the MDA value under vacuum was generally stable during 16-day storage. The PCC concentration under MAP1 and MAP2 after in vitro digestion was at the comparable level during the storage period. In summary, packaging and storage methods influenced the development of oxidation processes affecting the final quality of ostrich meat during storage. [ABSTRACT FROM AUTHOR]

Crystal structures of hemoglobin (Hb) from two flightless birds, ostrich (Struthio camelus) and turkey (Meleagris gallopova), were determined. The ostrich Hb structure was solved to a resolution of 2.22 Å, whereas two forms of turkey Hb were solved to resolutions of 1.66 Å (turkey monoclinic structure; TMS) and 1.39 Å (turkey orthorhombic structure; TOS). Comparison of the amino‐acid sequences of ostrich and turkey Hb with those from other avian species revealed no difference in the number of charged residues, but variations were observed in the numbers of hydrophobic and polar residues. Amino‐acid‐composition‐based computation of various physical parameters, in particular their lower inverse transition temperatures and higher average hydrophobicities, indicated that the structures of ostrich and turkey Hb are likely to be highly ordered when compared with other avian Hbs. From the crystal structure analysis, the liganded state of ostrich Hb was confirmed by the presence of an oxygen molecule between the Fe atom and the proximal histidine residue in all four heme regions. In turkey Hb (both TMS and TOS), a water molecule was bound instead of an oxygen molecule in all four heme regions, thus confirming that they assumed the aqua‐met form. Analysis of tertiary‐ and quaternary‐structural features led to the conclusion that ostrich oxy Hb and turkey aqua‐met Hb adopt the R‐/RH‐state conformation. [ABSTRACT FROM AUTHOR]

The Old Testament book of Job contains a "nature sermon," directly delivered by God Himself, unto Job, in answer to Job's questions about how Job's peaceful life was suddenly interrupted by agonizing afflictions and disasters. God's answers provide a creationist theology of God's goodness, might, wisdom, and providence. In this "nature sermon," reported in Job 38-41, God highlights His control over the physical cosmos, then He describes His control over the world of living creatures. Beginning in Job 38:39, God emphasizes how He has caringly planned for and carefully fitted resources for critical needs of His animal creatures, including reproductive success, dietary necessities, resources to facilitate terrestrial and aerial mobility, etc. Accordingly, (a) Job should recognize God's kind providence as it is wonderfully applied to God's lesser creatures; (b) Job should recognize that his own humanity is more valuable to God than the lives of wild animals; and (c) Job should patiently depend upon God to work out good outcomes for his life, here and hereafter. To teach these truths (which are later summarized in 1 Peter 4:19) unto Job, God provides these paired illustrations of various animal's needs and activities: (1) lion and raven; (2) wild mountain goat and cervid doe; (3) wild donkey and rhinoceros; (4) ostrich and horse; (5) hawk and eagle; (6) Behemoth and Leviathan. [ABSTRACT FROM AUTHOR]

The ostrich (Struthio camelus) is an herbivorous bird with a long and developed hindgut. In the hindgut, there is a dense and highly diverse population of anaerobic bacteria, and active fermentation produces high concentrations of short-chain fatty acids. Bacteria in the hindgut of the ostrich are considered vital for both their nutritional contribution and health benefits, such as benefits to the immune and defense system of the host. We attempted to isolate Lactobacillaceae, which might be involved in improving immune function and in inhibiting pathogens. The number of colonies from ostrich feces observed on LBS agar medium was 3.64×10³ per gram of feces. Three strains of Lactobacillaceae were isolated from the feces. Nearly the entire length of the 16S ribosomal RNA gene of these isolates was sequenced, and a homology search showed high identity with L. brevis (identity＝99.93%), L. coryniformis (98.39%), and L. paracasei (100.0%). These isolates may be deemed potential probiotics for the ostrich. [ABSTRACT FROM AUTHOR]

The Vercelli map has never been accorded its due place in the mappaemundi canon. Faced with problems of illegibility, few scholars have dared to research it. The availability of multispectral imaging (MSI), however, has provoked closer investigation. In this short note, attention is limited to the question of its date. Attempting to discover the genesis of a medieval map remains largely a matter of reasoned guesswork in stages. In this article, based on new assessments, a process of evolution is suggested that may enable us to reconcile the dating proposed more than hundred years ago by Carlo Errerà (1270–1285), half a century ago by Carlo Felice Capello (1191–1218) and, more recently, by Dan Terkla (1217). It is hoped that further MSI will challenge scholars to detect additional layers in the map. [ABSTRACT FROM AUTHOR]

A 25-year-old, male, sanctuary-owned, South African ostrich (Struthio camelus australis) was evaluated for orbital emphysema after evisceration of a nonvisual and chronically irritated eye. On initial ophthalmic examination, the ostrich's left eye displayed severe corneal fibrosis, broad anterior synechia, and a shallow anterior chamber, all suggestive of a previous corneal perforation. Conjunctival hyperemia and eyelid crusts were also present, reportedly associated with chronic rubbing. Evisceration of the left eye was performed by excising the eyelid margins, conjunctiva, nictitans, cornea, and intraocular contents. Four weeks postoperatively, a nonpainful, fluctuant swelling of the surgical site was noted. Trocarization of the surgical site verified orbital emphysema and served to temporarily decompress the orbit. Orbital emphysema reoccurred within 48 hours but gradually regressed without intervention over the subsequent 9 months. A Jones test was performed in the healthy, right eye and demonstrated clear communication to the oropharynx. To our knowledge, this is the first reported case of an evisceration performed on an ostrich and the first reported case of orbital emphysema in any avian species. It is probable that the emphysema noted in this case was secondary to nasolacrimal duct-oropharynx communication. [ABSTRACT FROM AUTHOR]

A 4-year-old female ostrich (Struthio camelus) developed knuckling on its left foot after a traumatic event. Conservative treatment, rest, and traditional splinting methods failed to resolve the gait abnormality or improve mobility. A dynamic multicomponent orthotic shoe was then custom designed and fabricated with an ultra-high–molecular weight, low-pressure polyethylene polymer (St 1000 DIN16972 TG2) to correct the foot malposition. The affected foot and leg were wrapped with soft padding and the orthosis was attached and secured by nylon belts (Nylon PA [polyamide] 6, a semicrystalline polyamide polymer) with self-locking modified wrench straps. The orthosis immediately prevented knuckling and improved ambulation. After 2 weeks, this shoe was replaced with a modified orthotic shoe of lighter weight to improve kinetics and ease of use by the bird. The modified orthosis dramatically improved ambulation and was left attached to the affected leg for 7 more weeks. Thereafter, the bird was able to ambulate comfortably without the aid of the shoe, even though permanent anatomical changes to the affected leg seemed to restrict its speed of locomotion. The ostrich had another unfortunate traumatic event 10 weeks later, the result of which was knuckling on the contralateral (right) foot. After routine medical and supportive care, the orthosis was then applied to the right foot for a period of 9 weeks. During this period the bird was able to ambulate well, despite having to support the bulk of its weight on the previously affected left limb. At the end of 9 weeks, the orthosis was removed, and the bird was able to ambulate without aid, although the bird's speed of locomotion was considerably reduced. After recovery of its leg problems, the ostrich was confined to a relatively small paddock for the rest of its life. [ABSTRACT FROM AUTHOR]

The study includes five adult male ostrich (Strothiue camelus). It was divided into proventriculus and gizzard the proventriculus was highly distended due to swallow a large mass of food anatomically the proventriculus had two regions papillary and non-papillary regions the proventriculus received food from esophagus via cardiac orifice. The wall of proventriculus was sharply marked off from the esophagus by color and nature of its wall by thickened ridge these structure characterized by colorless and slightly roughened the main function of proventriculus was production of gastric juice and propulsion of the food with these juice in the gizzard. The proventriculus connected with gizzard by region called isthmus. The gizzard was large muscular situated immediately to the keel bone resembled biconvex lence located between proventriculus and duodenum gizzard responsible for grinding of food. The inner surface of the gizzard is clearly distinguished by Horney lining cuticle. [ABSTRACT FROM AUTHOR]

The ostrich is the largest living bird and has unique characteristics in its locomotor system that differentiate it from other bird species. The purpose of this study was to provide a reference atlas of reference-interval computed tomography (CT) and cross-sectional anatomy of the tarsometatarsal region and digits in the ostrich (Struthio camelus). The pelvic limbs of 25 ostriches were used for this imaging study. The transverse CT images were obtained from the middle third of the tarsometatarsal bone to the distal end of the third digit. The specimens were frozen and sectioned with an electric band saw at 1.5–2 cm intervals. The CT images were compared with the corresponding frozen cross sections. The bones, ligaments, and tendons were identified and labeled at each 1.5–2 cm intervals. The CT images provided anatomic details of the tendons and ligaments in the tarsometatarsal region and digits of the ostrich. The transverse CT images provided an excellent depiction of the anatomic structures of the leg and foot when compared with the corresponding frozen cross sections. The information presented in this study may be used as an initial reference when evaluating the CT images of an ostrich's tarsometatarsal region and digits. Moreover, the information provided in this report may be helpful in determining definitive diagnoses of musculoskeletal disorders affecting the lower leg in this species. [ABSTRACT FROM AUTHOR]

This study describes the persistence of an embryonal structure through to sub‐adulthood in the ostrich and emu. Mandibles from sub‐adult ostrich and emu were subjected to special staining, light microscopy and dissected to reveal and describe Meckel's cartilage. Meckel's cartilage, composed of hyaline cartilage, was present within the neurovascular canal of both species. The persistence through to sub‐adulthood of Meckel's cartilage in the ostrich and emu is a feature not previously reported in any other avian species. The proximal end of Meckel's cartilage was ossified in the region of the articular bone and the distal end was ossified in some specimens. Although this structure may ossify at a much later stage in life, the function in young and sub‐adult birds may be to dampen shockwaves along the intramandibular nerve that result from the action of pecking. In the ostrich, the M. pseudotemporalis superficialis tendon inserted onto the supra‐angular bone and Meckel's cartilage. In the emu, a small portion of the tendon was attached to the supra‐angular bone and the main part to Meckel's cartilage. The persistence of Meckel's cartilage in adult lepidosaurs, crocodilians and ratites represents an unusual shared trait between the extant members of the above groups. [ABSTRACT FROM AUTHOR]

The ostrich meat is considered healthy as it contains low cholesterol and fat levels. Foodborne diseases caused by the bacterium Salmonellaare a significant public health concern. The study was conducted to determine the prevalence, the risk factors involved, and antimicrobial susceptibility patterns of Salmonella isolates recovered from captive ostrich feces in the district of Lahore, Pakistan. There is not a single research on this issue in Pakistan up to date in ostrich. A total of 100 ostrich fecal samples were collected-and specifically cultured on Salmonella Shigella agar. The antimicrobial susceptibility was checked for different antibiotics throughthe Kirby-Bauer disc diffusion method. The 21% fecal samples were found positive for Salmonella. The disease was found to besignificantly(p<0.05) associated with risk factors; water type(p=0.027), bird's capacity(p=0.042), cage hygiene(p=0.024), age of bird(p=0.041) andother bird species around (p=0.031) while enclosure size and diet were found non-significant. The bacterium was found highly susceptible to Amoxicillin followed by Chloramphenicol, Gentamicin, Cefotaxime, andEnrofloxacin respectively.Further Salmonella species-level diagnostic studies along with susceptibility checks of more therapeutic agents are suggested to future researchers. [ABSTRACT FROM AUTHOR]

Accurate diet formulations are required to fulfil the nutrient requirements of birds in order to achieve optimal production. Knowing how the skin, nodule and feather production characteristics vary with diets of different nutrient densities will help in least-cost modelling. Feather growth and nodule development are factors that were previously neglected in ostrich diet formulation, both of which are essential for the development of a predictive production model. In this trial, 120 birds were placed in 15 pens. Varying energy regimes (high, medium and low) and accompanying protein and amino acid profile levels (level 1–5) were assigned ad libitum to each pen. A randomly selected bird from each pen was slaughtered at 1, 35, 63, 103, 159, 168 and 244 days of age. During the slaughter, each bird was weighed, stunned, exsanguinated, defeathered and eviscerated. Feathers from four regions of the skin were plucked and weighed. The shaft diameter of the wing feathers was measured. The nodule size of the tanned skin was measured for each slaughter age. The data were transformed to natural logarithms and regressed against the total feather weight and the total featherless empty body protein weight to set up allometric growth equations. A prediction equation to determine nodule size of the live bird was proposed. Feed cost optimisation is paramount, and results from this study will aid in setting up least-cost optimisation (simulation) formulation models. [ABSTRACT FROM AUTHOR]

This study describes a sudden death in an ostrich (Struthio camelus) with a big neck. Grossly, the air sacs were thickened. Yellow to white, round or coalescent material was scattered on the air sacs. However, the cervical air sac was normal. Histopathologically, multinucleated giant cells, heterophils, and macrophages had infiltrated the air sacs, and many hyphae were seen in the air sacs and on their surfaces. Pyogranulomatous inflammation with intralesional hyphae was observed throughout the lungs. Aspergillus fumigatus was identified microbiologically. In conclusion, the affected animal died because of the respiratory disturbance caused by mycotic airsacculitis and pneumonia. [ABSTRACT FROM AUTHOR]

Morphological and morphometrical study of pelvic limb bones from adult birds viz ostrich (n=4), emu (n=6), domestic fowl (n=8) and duck (n=8) were carried out. Pubic symphysis was a distinctive feature of os coxae in ostrich. Medial surface of ilium was united to lumbosacral mass in ostrich and emu, whereas, only medial border made fusion in domestic fowl and duck. Post acetabular ilium was an elongated triangle in ostrich and emu, whereas, quadrilateral plate in domestic fowl and duck. Iliac fossae for kidneys were lacking in ostrich and emu. Ischium was a long rod in ostrich and emu. Ilioischiatic notch was present in ostrich and emu, while, ilio-ischiatic foramen was found in domestic fowl and duck. Caudal extremity of pubis was free in all the species studied excluding ostrich. Ostrich and emu exhibited a distinct pneumatic foramen on femur which was lacking in domestic fowl and duck. Length ratio of femur to tibiotarsus was 1:1.75, 1:1.88, 1:1.38 and 1:1.69 in ostrich, emu, domestic fowl and duck correspondingly. Lateral cnemial crest was a large tuberosity in ostrich; whereas, in other species it was a thick curved spine. Hypotarsus was in the form of spine in ostrich and emu. Bony spur of tarsometatarsus observed in male domestic fowl was lacking in the other species. Distal extremity showed two trochleae in ostrich, whereas, three trochleae, in the other species. Ostrich is the only didactyl bird with two digits. Emu possessed three digits, while, domestic fowl and duck possessed four. Our present study revealed pneumatic femur, longer tibiotarsus and tarsometatarsus and less number of digits in ostrich and emu. Reduction in number of phalanges also led to reduce the number of muscles and tendons associated to digits. The above mentioned features may aid ostrich and emu for high-speed locomotion. [ABSTRACT FROM AUTHOR]

Energy state and environmental impacts of production systems are of main criteria to reach sustainability. In the present research, ostrich was compared with a broiler production system based on the criteria. The required data was gathered in Boukan Township, West Azerbaijan, Iran, by a questioner method. The total input energy in ostrich and broiler systems was calculated as 150,419.81 MJ (1 ton of bird year)−1 and 344,579.58 MJ (1 ton of bird year)−1, respectively. Diesel fuel and feed (41.39% and 36.95%, respectively) in broiler and electricity (45.87%) in the ostrich production system had the highest energy shares. Eleven impact categories were estimated by SimaPro software in the studied systems. Except for human toxicity, fresh water aquatic ecotoxicity, and terrestrial ecotoxicity, the amounts of other environmental impacts in the ostrich production system were lower than those of the poultry production system. The amount of global warming indicator for the production of 1 ton of broiler chicken was 17,400 kg CO2 eq year−1 that was equal to 4350 kg CO2 eq period−1. This value was obtained as 1.68 × 104 kg CO2 eq year−1 for the production of ostrich meat. In both production systems, feed was the main factor responsible for almost all studied environmental impacts. [ABSTRACT FROM AUTHOR]

In this study, the ultrastructural development of germ cells in the ostrich embryo was analysed. The nuclear organisation and morphological characteristics of cytoplasm in the developing germ cells, on embryonic days 20, 26, and 36 and the day of hatching (5 samples from each stage) was analysed using transmission electron microscopy (TEM). Germ cells located in the cortex of left ovaries were identified by their large size and centrally located nucleus, with a conspicuous nucleolus. In these cells, the cytoplasm contained an abundance of mitochondria and free ribosomes. The structure of Balbiani body, a villous-like elevation in wide intercellular space and desmosome junction between two adjacent germ cells was also studied. The germ cells during embryonic development showed structural differences in both the nucleus and cytoplasm. [ABSTRACT FROM AUTHOR]

Ostrich (Struthio camelus) has become one of the valuable agricultural enterprises in many countries of the world. China has become Asian's number one ostrich species raiser with about 20,000 ostrich bred farms. China started in 1992 to import some ostrich species from South Africa. Ostrich was raised globely since the 1980s for its feathers, meat, egg, hair and bone are all of high economic value. China's progress in producing ostriches has improved it's rank from fifth to the second globely in recent years, due to the following factors: The area of pastures which reached 40% of the area of China.Large amounts of agricultural wasts. China has been producing iron and thus has produced the cheapest agricultural machines, such as irrigation machines, cutting and pressing the feed. China achieved progress in the electronical industries which have enabled it to produce hatchery machines to promote the poultry industry. China is making use of solar energy and producing cheap energy used to irrigate agricultural land and operate farm machinery. Thus, the price of feed is cheap and therefore it costs to feed the ostriches as little as possible.Use of seawater desalination technology and cultivation of saline-tolerant fodder crops such as fodder beat, bonicam and blubank .The use of leather processing tectology and production of the finest leather industries of shoes and bags .China has imported large amounts of ostriches from South Africa and used the best breeding and ostrich production systems in South Africa. The most important factors leading to the success of the ostrich industry in china are limited into three factors. First: Nutrition and cheap feed should be provided by planting enough alfalfa. Using solar energy to irrigate these lands. Sowing saline and drought tolerant genotypes, such as blubanks and bonecam, as well as machines for cutting and making pellet for green feed. Second: Hatching Third: Health. [ABSTRACT FROM AUTHOR]

Nematodes of the genus Libyostrongylus parasitize ostriches, causing high mortality rates. These nematodes are found in the proventriculus and ventriculus of ostriches, but little is known about their distribution and the possible anatomopathological changes they cause in the various regions of these organs. This paper describes the distribution and quantification of Libyostrongylus and pathological changes found in regions of the proventriculus and ventriculus of ostriches with high and low levels of both natural and experimental infection. Ostriches were necropsied and tissue samples from the distinct regions of both organs were analysed based on nematode counts and histopathology after staining with haematoxylin and eosin, Masson's trichrome or Alcian blue/PAS. The cranial and glandular regions of the proventriculus were the most parasitized. The ventriculus contained more nematodes in the caudal region. No macro- or microscopic pathological changes were observed in either of these organs of experimentally-infected birds. However, naturally-infected birds with high levels of infection presented proventriculus with macroscopic lesions and heterophilic infiltrates surrounding nematodes. In the glandular region of this organ, nematodes were located in the adenomeres of the secretory ducts, causing altered architecture and erosions and ulcerative lesions with damaged epithelium. Nematode eggs were found in the koilin layer of the middle and caudal regions of the ventriculus only of these birds. The pH of the regions assessed by Alcian blue/PAS staining changed from acidic in the proventriculus to more alkaline in the caudal region of the ventriculus. These data add knowledge to the biology of Libyostrongylus. RESEARCH HIGHLIGHTS The most parasitized areas were the cranial and glandular regions of the proventriculus. Naturally-infected birds with high levels of infection presented macro lesions in the proventriculus and damaged epithelium. Nematode eggs were found in the ventriculus. The proventriculus had an acidic pH, which turned alkaline towards the ventriculus. [ABSTRACT FROM AUTHOR]

In rapidly growing animals there are numerous selective pressures and developmental constraints underpinning the ontogenetic development of muscle-tendon morphology and mechanical properties. Muscle force generating capacity, tendon stiffness, elastic energy storage capacity and efficiency were calculated from muscle and tendon morphological parameters and in-vitro tendon mechanical properties, obtained from a growth series of ostrich cadavers. Ontogenetic scaling relationships were established using reduced major axis regression analysis. Ostrich pelvic limb muscle mass and crosssectional area broadly scaled with positive allometry, indicating maintained or relatively greater capacity for maximum isometric force generation in bigger animals. The length of distal limb tendons was found to scale with positive allometry in several tendons associated with antigravity support and elastic energy storage during locomotion. Distal limb tendon stiffness scaled with negative allometry with respect to body mass, with tendons being relatively more compliant in larger birds. Tendon material properties also appeared to be size-dependent, suggesting the relative increased compliance of tendons in larger ostriches is due in part to compensatory distortions in tendon material properties during maturation and development, not simply from ontogenetic changes in tendon geometry. Our results suggest that the previously reported increase in locomotor economy through ontogeny in the ostrich is likely due to greater potential for elastic energy storage with increasing body size. In fact, the rate of this increase may be somewhat greater than the conservative predictions of previous studies thus illustrating the biological importance of elastic tendon structures in adult ostriches. [ABSTRACT FROM AUTHOR]

In this study the morphological features of the optic tectum in ostrich embryo were studied macroscopically and microscopically. After gross anatomical study, fixed specimens of the optic lobes in 30th, 36th and 40th embryonic days were processed for paraffin sections. Sections were stained by Harris haematoxylin and eosin (H&E), Luxol Fast Blue/Cresyl Echt Violet and Malory PTAH dyes. The optic lobes had large volumes even on the 30th embryonic day and increased proportionally to age. The optic lobe consisted of two parts: gray matter (outer) and white matter (inner). The first external layer of the optic lobe e.g. molecular layer consisted of neural fibres, neuroglia and scarce small neurons. The most common appearance of the optic lobes was characterised by small to medium-sized neurons (rounded to pyramid-shaped with large and pale nucleus consistong of obvious nucleoli arranged in three layers whose thickness increased in the deeper one) supported by neuroglia. Larger size neurons and occasionally multipolar neurons were presented in the interior compared with these layers. The lateral mesencephalic nucleus was detectable in the optic lobe base even on 30th embryonic day and was composed of few multipolar neurons supported by neuroglia. The tectal ventricles were lined with simple cuboidal ciliated ependymal cells in the embryonic period. As embryonic age increased, the ratio of tectal ventricle volume to its thickness decreased. Special stainings showed that Nissl bodies and myelin fibres, also glial fibres were available from the 30th embryonic day and that their density, especially myelin fibres density, increased with age. [ABSTRACT FROM AUTHOR]

In ostriches, the population densities resulting from intensive rearing increases susceptibility to pathogens such as mycoplasmas. In addition to good management practices, vaccination offers an attractive alternative for controlling mycoplasma infections in food animals, instead of using antibiotics, which often leave unacceptable residues. The use of live attenuated vaccines, however, carry the concern of reversion to virulence or genetic recombination with field strains. Currently there are no commercially available vaccines against ostrich-infecting mycoplasmas and this study therefore set out to develop and evaluate the use of a DNA vaccine against mycoplasma infections in ostriches using an OppA protein as antigen. To this end, the oppA gene of " Mycoplasma nasistruthionis sp. nov." str. Ms03 was cloned into two DNA vaccine expression vectors after codon correction by site-directed mutagenesis. Three-months-old ostriches were then vaccinated intramuscularly at different doses followed by a booster vaccination after 6 weeks. The ability of the DNA vaccines to elicit an anti-OppA antibody response was evaluated by ELISA using the recombinant OppA protein of Ms03 as coating antigen. A statistically significant anti-OppA antibody response could be detected after administration of a booster vaccination indicating that the OppA protein was successfully immunogenic. The responses were also both dose and vector dependent. In conclusion, the DNA vaccines were able to elicit an immune response in ostriches and can therefore be viewed as an option for the development of vaccines against mycoplasma infections. [ABSTRACT FROM AUTHOR]