1. Delivery of miRNAs Using Porous Silicon Nanoparticles Incorporated into 3D Hydrogels Enhances MSC Osteogenesis by Modulation of Fatty Acid Signaling and Silicon Degradation.
- Author
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Shrestha S, Tieu T, Wojnilowicz M, Voelcker NH, Forsythe JS, and Frith JE
- Subjects
- Humans, Porosity, Dendrimers chemistry, Fatty Acid Synthase, Type I metabolism, Fatty Acid Synthase, Type I genetics, Core Binding Factor Alpha 1 Subunit metabolism, Core Binding Factor Alpha 1 Subunit genetics, Signal Transduction drug effects, MicroRNAs genetics, MicroRNAs metabolism, Mesenchymal Stem Cells metabolism, Mesenchymal Stem Cells cytology, Mesenchymal Stem Cells drug effects, Osteogenesis drug effects, Silicon chemistry, Hydrogels chemistry, Nanoparticles chemistry, Fatty Acids chemistry
- Abstract
Strategies incorporating mesenchymal stromal cells (MSC), hydrogels and osteoinductive signals offer promise for bone repair. Osteoinductive signals such as growth factors face challenges in clinical translation due to their high cost, low stability and immunogenicity leading to interest in microRNAs as a simple, inexpensive and powerful alternative. The selection of appropriate miRNA candidates and their efficient delivery must be optimised to make this a reality. This study evaluated pro-osteogenic miRNAs and used porous silicon nanoparticles modified with polyamidoamine dendrimers (PAMAM-pSiNP) to deliver these to MSC encapsulated within gelatin-PEG hydrogels. miR-29b-3p, miR-101-3p and miR-125b-5p are strongly pro-osteogenic and are shown to target FASN and ELOVL4 in the fatty acid biosynthesis pathway to modulate MSC osteogenesis. Hydrogel delivery of miRNA:PAMAM-pSiNP complexes enhanced transfection compared to 2D. The osteogenic potential of hBMSC in hydrogels with miR125b:PAMAM-pSiNP complexes is evaluated. Importantly, a dual-effect on osteogenesis occurred, with miRNAs increasing expression of alkaline phosphatase (ALP) and Runt-related transcription factor 2 (RUNX2) whilst the pSiNPs enhanced mineralisation, likely via degradation into silicic acid. Overall, this work presents insights into the role of miRNAs and fatty acid signalling in osteogenesis, providing future targets to improve bone formation and a promising system to enhance bone tissue engineering., (© 2024 The Authors. Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
- Published
- 2024
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