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Start Over Descriptor "Titanium surfaces" Topic osteoblasts
10 results on '"Titanium surfaces"'

1. Osteoblastic differentiating potential of dental pulp stem cells in vitro cultured on a chemically modified microrough titanium surface.

Author

DE Colli M, Radunovic M, Zizzari VL, DI Giacomo V, DI Nisio C, Piattelli A, Calvo Guirado JL, Zavan B, Cataldi A, and Zara S

Subjects
Acid Etching, Dental, Calcium chemistry, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cell Survival, Cells, Cultured, Dental Pulp metabolism, Dinoprostone metabolism, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Humans, In Vitro Techniques, Interleukin-6 metabolism, Magnesium chemistry, Materials Testing, Microscopy, Electron, Scanning, Polymerase Chain Reaction, Stem Cells metabolism, Surface Properties, Dental Pulp cytology, Osteoblasts cytology, Stem Cells cytology, Titanium chemistry
Abstract

Titanium surface modification is critical for dental implant success. Our aim was to determine surfaces influence on dental pulp stem cells (DPSCs) viability and differentiation. Implants were divided into sandblasted/acid-etched (control) and sandblasted/acid-etched coated with calcium and magnesium ions (CaMg), supplied as composite (test). Proliferation was evaluated by MTT, differentiation checking osteoblastic gene expression, PGE2 secretion and matrix formation, inflammation by Interleukin 6 (IL-6) detection. MTT and IL-6 do not modify on test. A PGE2 increase on test is recorded. BMP2 is higher on test at early experimental points, Osterix and RUNX2 augment later. Alizarin-red S reveals higher matrix production on test. These results suggest that test surface is more osteoinductive, representing a start point for in vivo studies aiming at the construction of more biocompatible dental implants, whose integration and clinical performance are improved and some undesired effects, such as implant stability loss and further surgical procedures, are reduced.

Published
2018
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2. Nd:YAG laser radiation (1.064 nm) accelerates differentiation of osteoblasts to osteocytes on smooth and rough titanium surfaces in vitro.

Author

Karoussis IK, Kyriakidou K, Psarros C, Lang NP, and Vrotsos IA

Subjects
Cell Adhesion radiation effects, Cells, Cultured, In Vitro Techniques, Microscopy, Electron, Scanning, Surface Properties, Cell Differentiation radiation effects, Cell Proliferation radiation effects, Lasers, Solid-State, Low-Level Light Therapy instrumentation, Osteoblasts radiation effects, Titanium chemistry
Abstract

Background: A number of studies revealed beneficial effects of low-level laser therapy (LLLT) regarding cell proliferation and differentiation., Aim: To investigate the effect of Nd:YAG (1.064 nm) laser radiation in the proliferation and differentiation potential of MG-63 osteoblast-like cells. Additionally, the effects of the surface configurations were to be evaluated., Material and Methods: MG-63 osteoblast cells were cultured on different surfaces: plastic tissue culture, smooth (polished) titanium-PT and rough titanium-SLA. The effects of both titanium surfaces and low-level laser therapy (LLLT) on cell adhesion were evaluated by the gene expression of molecules involved in cell proliferation and differentiation. In addition, scanning electron microscopy (SEM) and MTT proliferation assays were used to examine cell morphology and proliferation, respectively., Results: Compared to smooth (PT) surfaces, SLA surfaces favoured MG-63 cell differentiation. Following the application of Nd:YAG laser irradiation, cells yielded statistically significantly improved differentiation on both smooth and SLA surfaces compared with non-irradiated surfaces., Conclusions: The findings of this present study suggest that both surface morphology and Nd:YAG laser irradiation influence the proliferation and differentiation potential of MG-63 cells., (© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)

Published
2017
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3. Effects of Titanium Surface Microtopography and Simvastatin on Growth and Osteogenic Differentiation of Human Mesenchymal Stem Cells in Estrogen-Deprived Cell Culture.

Author

Arpornmaeklong, Premjit, Pripatnanont, Prisana, Chookiatsiri, Chonticha, and Tangtrakulwanich, Boonsin

Subjects
SIMVASTATIN, STEM cells, OSTEOBLASTS, TITANIUM, ESTROGEN, GENE expression, PHYSIOLOGY, CELL differentiation, POLYMERASE chain reaction
Abstract

Purpose: This study aimed to investigate the effects of titanium surface topography and simvastatin on growth and osteogenic differentiation of human bone marrow stromal cells (hBMSCs) in estrogen-deprived (ED) cell culture. Materials and Methods: Human BMSCs were seeded on cell culture plates, smooth-surface titanium (Ti) disks, and sandblasted with large grits and acid etched (SLA)–surface Ti disks; and subsequently cultured in regular (fetal bovine serum [FBS]), ED, and ED-with 100 nM simvastatin (ED-SIM) culture media for 14 to 21 days. Live/dead cell staining, scanning electron microscope examination, and cell viability assay were performed to determine cell attachment, morphology, and growth. Expression levels of osteoblast-associated genes, Runx2 and bone sialoprotein and levels of alkaline phosphatase (ALP) activity, calcium content, and osteocalcin in culture media were measured to determine osteoblastic differentiation. Expression levels of bone morphogenetic protein-2 (BMP-2) were investigated to examine stimulating effects of simvastatin (n = 4 to 5, mean ± SD). In vitro mineralization was verified by calcein staining. Results: Human BMSCs exhibited different attachment and shapes on smooth and SLA titanium surfaces. Estrogen-deprived cell culture decreased cell attachment and growth, particularly on the SLA titanium surface, but cells were able to grow to reach confluence on day 21 in the ED-osteogenic (OS) culture medium. Promoting effects of the SLA titanium surface in ED-OS were significantly decreased. Simvastatin significantly increased osteogenic differentiation of human BMSCs on the SLA titanium surface in the ED-OS medium, and the promoting effects of simvastatin corresponded with the increasing of BMP-2 gene expression on the SLA titanium surface in ED-OS-SIM culture medium. Conclusion: The ED cell culture model provided a well-defined platform for investigating the effects of hormones and growth factors on cells and titanium surface interaction. Titanium, the SLA surface, and simvastatin synergistically promoted osteoblastic differentiation of hBMSCs in ED condition and might be useful to promote osteointegration in osteoporotic bone. [ABSTRACT FROM AUTHOR]

Published
2017
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4. Nd: YAG laser radiation (1.064 nm) accelerates differentiation of osteoblasts to osteocytes on smooth and rough titanium surfaces in vitro.

Author

Karoussis, Ioannis K., Kyriakidou, Kyriaki, Psarros, Costas, Lang, Niklaus P., and Vrotsos, Ioannis A.

Subjects
LOW-level radiation, CELL differentiation, OSTEOBLASTS, GENE expression, CELL adhesion, OSSEOINTEGRATED dental implants
Abstract

Background A number of studies revealed beneficial effects of low-level laser therapy ( LLLT) regarding cell proliferation and differentiation. Aim To investigate the effect of Nd: YAG (1.064 nm) laser radiation in the proliferation and differentiation potential of MG-63 osteoblast-like cells. Additionally, the effects of the surface configurations were to be evaluated. Material and methods MG-63 osteoblast cells were cultured on different surfaces: plastic tissue culture, smooth (polished) titanium- PT and rough titanium- SLA. The effects of both titanium surfaces and low-level laser therapy ( LLLT) on cell adhesion were evaluated by the gene expression of molecules involved in cell proliferation and differentiation. In addition, scanning electron microscopy ( SEM) and MTT proliferation assays were used to examine cell morphology and proliferation, respectively. Results Compared to smooth ( PT) surfaces, SLA surfaces favoured MG-63 cell differentiation. Following the application of Nd: YAG laser irradiation, cells yielded statistically significantly improved differentiation on both smooth and SLA surfaces compared with non-irradiated surfaces. Conclusions The findings of this present study suggest that both surface morphology and Nd: YAG laser irradiation influence the proliferation and differentiation potential of MG-63 cells. [ABSTRACT FROM AUTHOR]

Published
2017
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5. A Key Role of Autophagy in Osteoblast Differentiation on Titanium-Based Dental Implants.

Author

Kaluđerović, Milena R., Mojić, Marija, Schreckenbach, Joachim P., Maksimović-Ivanić, Danijela, Graf, Hans-Ludwig, and Mijatović, Sanja

Subjects
*OSTEOBLASTS, *AUTOPHAGY, *TITANIUM, *DENTAL implants, *HOMEOSTASIS, *FLOW cytometry, *BIOMINERALIZATION
Abstract

Autophagy plays an important role in embryogenesis, for the maintenance of tissue homeostasis and the elimination of damaged subcellular structures. Furthermore, autophagy could be a mode of physiological cell death and also be implicated in cell differentiation. Thus, we hypothesized that autophagy may have an impact on the differentiation of osteoblast cells influenced by various titanium-based surfaces. Interactions between smooth, commercially available pure titanium (Ti cp), rough Ticer, acid-etched Ti cp (SS) and M1-M3 (comprised of the monoclinic phase of sodium-titanium oxides and rutile; M2 contains amorphous calcium phosphates) and human osteoblast cells were investigated. Immunofluorescent staining was used for detecting autophagy, cell cluster formation and collagen type I (Col-1) expression. Flow cytometry was employed to identify autophagy, the production of endogenous nitric oxide (NO) and the size and granularity of the cells. Rough surfaces caused osteoblast differentiation via the autophagic-dependent PI3/Akt signalling pathway. These surfaces induced the formation of discrete populations of large, granular cells, i.e. mature osteoblasts. In addition, M1-M3 provoked the development of a third population of small, granular cells, responsible for cell cluster formation, which are important for the formation of bone noduli and mineralisation. The same surfaces induced faster osteoblast maturation and enhanced NO production, a hallmark of the already mentioned processes. Neither the mature osteoblasts nor the small cells appeared after the inhibition of autophagy. Inhibition of autophagy also prevented cell cluster formation. We demonstrate that autophagy plays an essential role in the osteoblast differentiation on titanium-based surfaces with rough topography. © 2015 S. Karger AG, Basel [ABSTRACT FROM AUTHOR]

Published
2015
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6. Behavior of SaOS-2 Cells Cultured on Different Titanium Surfaces.

Author

Postiglione, L., Domenico, G. Di, Ramaglia, L., Montagnani, S., Salzano, S., Meglio, F. Di, Sbordone, L., Vitale, M., and Rossi, G.

Subjects
DENTAL metallurgy, GROWTH factors, DENTAL implants, TITANIUM, BONE cells, DENTAL matrices
Abstract

Surface properties may affect the clinical outcome of titanium dental implants. The aim of the present study was to investigate the effects of 3 different titanium surfaces—smooth (S), sandblasted (SB), and titanium plasma-sprayed (TPS)—on proliferation, differentiation, and apoptosis of human osteoblast-like cells, SaOS-2. Cell proliferation was significantly (p < 0.05) higher on the S surface, and synthesis of extracellular matrix proteins was more abundant on TPS and SB than on S surfaces. Analysis of integrin receptors showed a higher expression of α2, α5, αVβ3, and ß1 on TPS as compared with SB and S surfaces. An increase in alkaline phosphatase activity was detected only on SB and TPS surfaces. Analysis of cell apoptosis did not demonstrate any significant difference among the 3 different surfaces. The results indicate that titanium surface topography affects proliferation and differentiation of osteoblast-like SaOS-2 cells, suggesting that surface properties might be important for bone response around dental implants in vivo. [ABSTRACT FROM AUTHOR]

Published
2003
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7. Titanium surfaces with adherent selenium nanoclusters as a novel anticancer orthopedic material

Author

Phong A. Tran, Thomas J. Webster, Robert H. Hurt, and Love Sarin

Subjects
Nanostructure, implant, cancer inhibition, X ray photoelectron spectroscopy, Metal Nanoparticles, Implant-tissue interfaces, Cancer growth, In-vitro, Orthopedic implant, Mice, Titanium substrates, bone cell, Neoplasms, Materials Testing, Bone cell, Bone cancer, Nanotechnology, Chemopreventive, Cancer, Titanium, atomic force microscopy, Osteoblast, biomaterial, Metals and Alloys, Biomaterial, Nanoclusters, scanning electron microscope, Stress shielding, protein content, enzyme activity, Surfaces, medicine.anatomical_structure, material coating, Bone cells, Alkaline phosphatase activity, AFM, Cell death, in vitro study, Materials science, Titanium surfaces, Biomedical Engineering, chemistry.chemical_element, Antineoplastic Agents, antineoplastic activity, Bone and Bones, Osseointegration, sodium selenite, Glutathiones, Biomaterials, Selenium, Antieoplastic Agents, Elemental selenium, XPS, Cell Adhesion, medicine, Calcium deposition, Animals, Humans, controlled study, human, Bone, Active agents, Cell Proliferation, cell culture, calcium, Osteoblasts, human cell, SEM image, Phosphatases, 090000 ENGINEERING, Orthopedic, Biological materials, Water, Alkaline Phosphatase, 090300 BIOMEDICAL ENGINEERING, Orthopedic materials, Nanostructured composites, cell differentiation, Orthopedics, chemistry, Wear debris, 090301 Biomaterials, High-density, Microscopy, Electron, Scanning, Ceramics and Composites, cell function, Debris, Surface density, cell density, Biomedical engineering
Abstract

Current orthopedic implants have several problems that include poor osseointegration for extended periods of time, stress shielding and wear debris-associated bone cell death. In addition, numerous patients receive orthopedic implants as a result of bone cancer resection, yet current orthopedic materials were not designed to prevent either the occurrence or reoccurrence of cancer. The objective of this in vitro study was to create a new biomaterial which can both restore bone and prevent cancer growth at the implant-tissue interface. Elemental selenium was chosen as the biologically active agent in this study because of its known chemopreventive and chemotherapeutic properties. It was found that when selenite salts were reduced by glutathione in the presence of an immersed titanium substrate, elemental selenium nucleated and grew into adherent, hemispherical nanoclusters that formed a nanostructured composite surface. Three types of surfaces with different selenium surface densities on titanium were fabricated and confirmed by SEM images, AFM, and XPS profiles. Compared to conventional untreated titanium, a high-density selenium-doped surface inhibited cancerous bone cell proliferation while promoting healthy bone cell functions (including adhesion, proliferation, alkaline phosphatase activity and calcium deposition). These findings showed for the first time the potential of selenium nanoclusters as a chemopreventive titanium orthopedic material coating that can also promote healthy bone cell functions.

Published
2009
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8. Comparison of human mandibular osteoblasts grown on two commercially available titanium implant surfaces

Author

Stefano Guizzardi, Desiree Martini, Guido Maria Macaluso, Giovanni Mauro, Carlo Galli, Giovanni Passeri, Anna Tinti, C. Galli, S. Guizzardi, G. Passeri, D. Martini, A. Tinti, G. Mauro, and G.M. Macaluso

Subjects
Time Factors, Surface Properties, Osteocalcin, chemistry.chemical_element, Dentistry, Receptors, Cytoplasmic and Nuclear, Mandible, Cell morphology, Spectrum Analysis, Raman, Receptors, Tumor Necrosis Factor, Dental Materials, Osteoprotegerin, Acid Etching, Dental, Coated Materials, Biocompatible, Cell Adhesion, Humans, Cell adhesion, TITANIUM SURFACES, Cell Shape, Cell Proliferation, Glycoproteins, Dental Implants, Titanium, RAMAN MICROSPECTROSCOPY, Osteoblasts, biology, Chemistry, business.industry, Proteins, Cell Differentiation, Adhesion, Alkaline Phosphatase, Air Abrasion, Dental, SEM, biology.protein, Microscopy, Electron, Scanning, Periodontics, Alkaline phosphatase, Adsorption, business, Protein adsorption, Biomedical engineering
Abstract

Surface characteristics play a major role in determining tissue response to implants and therefore their clinical outcome. The aim of the present study was to compare two commercially available titanium surfaces: plasma sprayed (TPS) and sand-blasted, acid-etched surface (SLA). METHODS: The surfaces were characterized by roughness testing, scanning electronic microscopy (SEM), Raman spectroscopy, and protein adsorption to determine their microtopographic and chemical properties. The effect of the surfaces on human mandibular osteoblasts was then studied in terms of cell morphology, adhesion, proliferation, and differentiation. Human osteoblasts from the mandible were cultured on these two surfaces and evaluated at 3, 6, 24, and 48 hours to determine cell attachment and morphology. Growth and differentiation kinetics were subsequently investigated by evaluating cell growth, alkaline phosphatase activity, osteocalcin and osteoprotegerin production at 7, 14, and 21 days. RESULTS: Although roughness was quite similar, the two surfaces presented strong differences in their topography, and cell morphology varied as a consequence. Osteoblasts on SLA appeared more elongated and spindle shaped than those on TPS, and their adhesion at 3 and 6 hours was weaker, but reached that of cells on TPS at hour 24. Cell proliferation was greater on SLA surfaces but differentiation parameters; i.e., alkaline phosphatase and osteocalcin, provided better results on TPS surfaces. Osteoprotegerin production was enhanced on TPS surfaces at days 14 and 21. CONCLUSION: Although cells grown on both surfaces exhibited good adhesion capabilities, a well-differentiated osteoblastic phenotype, and maintained a clear proliferation potential, our study suggests that plasma-sprayed treatment offers a better performance than SLA by creating, at least in the early phases, better conditions for tissue healing.

Published
2005

9. Bone response to a Ca- and P-Enriched titanium surface obtained by anodization

Author

Adalberto Luiz Rosa, Paulo Tambasco de Oliveira, Rosemeire de Lordo Franco, Roberto Chiesa, and Marcio Mateus Beloti

Subjects
anodization, implant, Medullary cavity, Surface Properties, Connective tissue, chemistry.chemical_element, Osteoclasts, Matrix (biology), bone, Bone Response, Dental Materials, Dogs, Coated Materials, Biocompatible, Bone Marrow, Osseointegration, Osteogenesis, medicine, Animals, General Dentistry, Dental Implants, Titanium, Osteoblasts, Anodizing, Chemistry, Phosphorus, Humerus, Electroplating, calcium-phosphate coating, titanium surfaces, Oxygen, medicine.anatomical_structure, Dental Prosthesis Design, Connective Tissue, Models, Animal, Microscopy, Electron, Scanning, Titanium surface, Calcium, Implant, Bone Remodeling, Collagen, Porosity, Biomedical engineering, Electron Probe Microanalysis
Abstract

This study evaluated bone response to a Ca- and P- enriched titanium (Ti) surface treated by a multiphase anodic spark deposition coating (BSP-AK). Two mongrel dogs received bilateral implantation of 3 Ti cylinders (4.1 x 12 mm) in the humerus, being either BSP-AK treated or untreated (machined - control). At 8 weeks postimplantation, bone fragments containing the implants were harvested and processed for histologic and histomorphometric analyses. Bone formation was observed in cortical area and towards the medullary canal associated to approximately 1/3 of implant extension. In most cases, in the medullary area, collagen fiber bundles were detected adjacent and oriented parallel to Ti surfaces. Such connective tissue formation exhibited focal areas of mineralized matrix lined by active osteoblasts. The mean percentages of bone-to-implant contact were 2.3 (0.0-7.2 range) for BSP-AK and 0.4 (0.0-1.3 range) for control. Although the Mann-Whitney test did not detect statistically significant differences between groups, these results indicate a trend of BSP-AK treated surfaces to support contact osteogenesis in an experimental model that produces low bone-to-implant contact values. O objetivo desse estudo foi avaliar a resposta do tecido ósseo à superfície de titânio (Ti) enriquecida com Ca e P obtida por anodização (BSP-AK). Três cilindros de Ti (4,1 x 12 mm) BSP-AK ou usinado (controle) foram implantados bilateralmente nos úmeros de dois cães de raça indefinida. Oito semanas após a implantação, os fragmentos ósseos contendo os implantes foram removidos e processados para análises histológica e histomorfométrica. A formação óssea foi observada na região cortical e no canal medular até aproximadamente um terço da extensão do implante. Na maioria dos casos, feixes de fibras colágenas dispostos paralelamente à superfície do implante foram observados na região medular. Nessa região observaram-se também áreas focais de formação de matriz mineralizada e osteoblastos ativos. Os implantes do grupo BSP-AK apresentaram média de contato osso-implante 2,3%, com medidas variando de 0,0 a 7,2% e os do grupo controle tiveram média 0,4%, com medidas variando de 0,0 a 1,3%. Apesar do teste de Mann-Whitney não mostrar diferença estatisticamente significante entre os grupos, nossos resultados indicaram uma tendência para a ocorrência de osteogênese de contato na superfície BSP-AK em um modelo experimental que resulta em baixos valores de contato osso-implante.

10. Titanium surface decorated with selenium nanoclusters - A novel promising material for orthopedic application

Author

Sarin, L., Phong Tran, Webster, T. J., and Hurt, R. H.

Subjects
Nanostructure, implant, cancer inhibition, X ray photoelectron spectroscopy, Metal Nanoparticles, Implant-tissue interfaces, In-vitro, Orthopedic implant, Biological meterials, Mice, bone cell, Neoplasms, Bone cancer, Nanotechnology, Sodium selenite, Chemopreventive, Cell proliferation, Cancer, Titanium, atomic force microscopy, Osteoblast, biomaterial, Nanoclusters, scanning electron microscope, protein content, enzyme activity, Surfaces, material coating, Bone cells, Alkaline phosphatase activity, AFM, alkaline phosphatase, Cell death, in vitro study, Titanium surfaces, Antineoplastic Agents, antineoplastic activity, Stress shielding, Bone and Bones, Glutathiones, Selenium, Osseointegration, Elemental selenium, XPS, Cell Adhesion, Calcium deposition, Animals, controlled study, human, Bone, Active agents, cell culture, calcium, Osteoblasts, human cell, SEM image, Ttianium substrates, Phosphatases, 090000 ENGINEERING, Orthopedic, Water, 090300 BIOMEDICAL ENGINEERING, Orthopedic materials, Nanostructured composites, cell differentiation, Orthopedics, Wear debris, 090301 Biomaterials, High-density, Microscopy, Electron, Scanning, cell function, Debris, Surface density, cell density, Materials testing

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