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Start Over Author "Heer AH" Topic osteoarthritis
2 results on '"Heer AH"'

1. Comparative analysis of cathepsin L, cathepsin D, and collagenase messenger RNA expression in synovial tissues of patients with rheumatoid arthritis and osteoarthritis, by in situ hybridization.

Author

Keyszer GM, Heer AH, Kriegsmann J, Geiler T, Trabandt A, Keysser M, Gay RE, and Gay S

Subjects
Arthritis, Rheumatoid metabolism, Arthritis, Rheumatoid pathology, Cartilage, Articular pathology, Cathepsin D biosynthesis, Cathepsin D genetics, Cathepsin L, Cathepsins biosynthesis, Cell Adhesion, Collagenases biosynthesis, Cysteine Endopeptidases biosynthesis, Cysteine Endopeptidases genetics, Gene Expression Regulation, Enzymologic, Humans, In Situ Hybridization, Osteoarthritis metabolism, Osteoarthritis pathology, RNA, Messenger analysis, RNA, Messenger biosynthesis, RNA, Messenger genetics, Synovial Membrane pathology, Synovial Membrane physiology, Arthritis, Rheumatoid enzymology, Cathepsins genetics, Collagenases genetics, Endopeptidases, Osteoarthritis enzymology, Synovial Membrane enzymology
Abstract

Objective: To compare the expression of cathepsin L, cathepsin D, and collagenase messenger RNA (mRNA) in synovial specimens from patients with rheumatoid arthritis (RA) and osteoarthritis (OA)., Methods: The expression of cathepsins L and D as well as collagenase mRNA in synovial tissues from 8 patients with RA, 6 patients with OA, and 2 patients with noninflamed joints was evaluated using in situ hybridization with digoxigenin-labeled RNA probes., Results: Both RA and OA synovial tissue expressed cathepsins L and D as well as collagenase mRNA. The expression of the cathepsins was markedly higher in interstitial regions and, to some extent, in perivascular infiltrates of RA synovial tissue compared with OA specimens., Conclusion: Cathepsins L and D mRNA are expressed differently in RA and OA synovial tissues, supporting the concept that these enzymes may contribute to the influx of mononuclear cells into RA synovium. Moreover, the data reveal that the expression of collagenase and cathepsins in RA and OA synovial lining is otherwise largely similar, and suggest that the adhesion of synovial cells to cartilage mediates the invasive destructive process in RA.

Published
1995
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2. Detection of insulin-like growth factor I and II in synovial tissue specimens of patients with rheumatoid arthritis and osteoarthritis by in situ hybridization.

Author

Keyszer GM, Heer AH, Kriegsmann J, Geiler T, Keysser C, Gay RE, and Gay S

Subjects
Arthritis, Rheumatoid pathology, Humans, In Situ Hybridization, Insulin-Like Growth Factor I genetics, Insulin-Like Growth Factor II genetics, RNA, Messenger metabolism, Synovial Membrane pathology, Arthritis, Rheumatoid metabolism, Insulin-Like Growth Factor I metabolism, Insulin-Like Growth Factor II metabolism, Osteoarthritis metabolism, Synovial Membrane metabolism
Abstract

Objective: To study the expression of insulin-like growth factor I and II (IGF I and II) in synovial tissue specimen of patients with rheumatoid arthritis (RA) and osteoarthritis (OA)., Methods: Synovial tissue sections were examined for the expression of IGF I and II by in situ hybridization using digoxigenin labeled antisense and sense RNA probes., Results: The antisense probe of IGF I reacted with all specimens. IGF II mRNA was expressed in 7/7 RA and 4/5 OA tissues. Cells of the synovial lining and subsynovial layer bound both antisense probes, whereas inflammatory infiltrates of RA tissues were labeled rarely., Conclusion: The significant number of cells in the synovium that express IGF I and II mRNA suggests a role of IGF in repair mechanisms of articular cartilage in response to injury and effects on fibroblast growth within the synovium.

Published
1995

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