1. Engineered 3D-Vessels-on-Chip to study effects of dynamic fluid flow on human induced pluripotent stem cell derived endothelial cells
- Author
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Graaf, M.N.S. de, Mummery, C.L., Orvola, V.V., Passier, P.C.J.J., Berg, A. van den, Maagenberg, A.M.J.M. van den, Peters, D.J.M., Meer, A.D. van der, Mastrangeli, M., Meer, B.J. van, and Leiden University
- Subjects
Microphysiological system ,Hydrogel ,Microvasculature ,Haemodynamics ,Organ-on-Chip ,Induced pluripotent stem cell ,Endothelial cells ,Microfluidics ,Tissue engineering ,Microfabrication - Abstract
To improve the predictive capability of pre-clinical models and reduce the use of animal models in drug discovery and disease modelling, advanced in vitro models are being developed. These microphysiological systems (MPS) or “Organs-on-Chip” (OoC) are being developed to include all aspects of the human physiology to improve the in vitro cellular response. OoCs combined with differentiated human induced pluripotent stem cells (hiPSC) allow the use of cells with patient specific genotypes and aid the development of personalized and precision medicine.In this thesis, the development of tractable models of the vasculature is described. These models allow for the combination of hiPSC-derived vascular and tissue specific cells with haemodynamics to recapitulate essential stimuli of blood vessels.
- Published
- 2023