1. Varifocal Metalens for Optical Sectioning Fluorescence Microscopy
- Author
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Yi-You Huang, Din Ping Tsai, Hiroaki Misawa, Xu Shi, Cheng Hung Chu, Yu Hsin Chia, Mu Ku Chen, Sunil Vyas, Yuan Luo, Takuo Tanaka, and Hsin Yu Kuo
- Subjects
Fluorescence-lifetime imaging microscopy ,Materials science ,Optical sectioning ,Bioengineering ,02 engineering and technology ,law.invention ,Mice ,Optics ,law ,Microscopy ,Fluorescence microscope ,Animals ,Focal length ,General Materials Science ,Lighting ,Lenses ,business.industry ,Mechanical Engineering ,Endoscopy ,General Chemistry ,Moiré pattern ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Lens (optics) ,Spherical aberration ,Microscopy, Fluorescence ,0210 nano-technology ,business - Abstract
Fluorescence microscopy with optical sectioning capabilities is extensively utilized in biological research to obtain three-dimensional structural images of volumetric samples. Tunable lenses have been applied in microscopy for axial scanning to acquire multiplane images. However, images acquired by conventional tunable lenses suffer from spherical aberration and distortions. Here, we design, fabricate, and implement a dielectric Moiré metalens for fluorescence imaging. The Moiré metalens consists of two complementary phase metasurfaces, with variable focal length, ranging from ∼10 to ∼125 mm at 532 nm by tuning mutual angles. In addition, a telecentric configuration using the Moiré metalens is designed for high-contrast multiplane fluorescence imaging. The performance of our system is evaluated by optically sectioned images obtained from HiLo illumination of fluorescently labeled beads, as well as ex vivo mice intestine tissue samples. The compact design of the varifocal metalens may find important applications in fluorescence microscopy and endoscopy for clinical purposes.
- Published
- 2021
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