1. End-specific covalent photo-dependent immobilisation of synthetic DNA to paramagnetic beads.
- Author
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Penchovsky R, Birch-Hirschfeld E, and McCaskill JS
- Subjects
- Chromatography, High Pressure Liquid, Cross-Linking Reagents chemistry, Nucleic Acid Hybridization, Oligonucleotide Probes genetics, Oligonucleotides genetics, Photochemistry, Polystyrenes, Silica Gel, Silicon Dioxide, Nitrophenols chemistry, Oligonucleotides chemistry, Pyruvates chemistry
- Abstract
A novel approach for light-dependent covalent immobilisation of synthetic DNA oligomers to amino-coated paramagnetic beads is described. A hetero-bifunctional photo-reactive cross-linking chemical, 4-nitrophenyl 3-diazopyruvate, is applied to attach 5' amino-modified DNA to both silica and polystyrene paramagnetic beads. The coupling yields are comparable with similar methods in which no photo-reactive chemicals are used. The immobilised DNA on the polystyrene and silica beads was used efficiently in hybridisation experiments. An extension of this approach to light-directed immobilisation of specific DNA to beads, located at different positions in micro-flow reactors, opens up a range of integrated applications to complex diagnostics, evolutionary biotechnology and novel areas such as DNA computing.
- Published
- 2000
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