1. Prostaglandins have no effect on spontaneous and gonadotrophin-releasing hormone stimulated luteinizing hormone release in cultured rat pituitary cells
- Author
-
Günter Emons, Sabine Thiessen, and Ulrich Gethmann
- Subjects
medicine.medical_specialty ,Prostaglandin ,Gonadotropic cell ,chemistry.chemical_compound ,Internal medicine ,Culture Techniques ,Gonadotrophin releasing hormone ,medicine ,Animals ,Dose-Response Relationship, Drug ,business.industry ,Cellular Regulation ,Obstetrics and Gynecology ,Rats, Inbred Strains ,General Medicine ,Luteinizing Hormone ,Stimulation, Chemical ,Pituicyte ,Rats ,Rat Pituitary ,Endocrinology ,chemistry ,Pituitary Gland ,Prostaglandins ,lipids (amino acids, peptides, and proteins) ,Female ,Luteinizing hormone ,business ,Pituitary Hormone-Releasing Hormones ,Hormone - Abstract
Primary cultures of pituitary cells from adult female rats were exposed to increasing concentrations (10(-13) to 10(-6)M) of selected prostaglandins (PGE2, PGF2 alpha, PGI2, 6-keto-PGF1 alpha, and sulproston). The release of luteinizing hormone (LH) by the pituicytes was monitored. In a second series of experiments, pituitary cells were treated with prostaglandins as described above and additionally challenged with a submaximal stimulus of gonadotrophin-releasing hormone (GnRH; 10(-9) M). The spontaneous LH-release in all prostaglandin treated cultures did not differ from the controls. When stimulated with 10(-9) M GnRH, the LH-release increased approx. six-fold in all cultures, with no difference between prostaglandin treated cells and the respective controls. Thus, the prostaglandins tested - at least at concentrations less than or equal to 10(-6) M - have neither a direct positive or negative effect on pituitary LH-release, nor do they enhance or decrease the LH-releasing effect of GnRH. These prostaglandins are probably not involved in the cellular regulation of LH-release by the pituicyte.
- Published
- 1984