4 results on '"Conceição, L. E. C."'
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2. Optimal dietary protein levels in juvenile Senegalese sole ( Solea senegalensis).
- Author
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Rema, P., Conceição, L. E. C., Evers, F., Castro-Cunha, M., Dinis, M. T., and Dias, J.
- Subjects
- *
LOW-protein diet , *BODY weight , *AQUACULTURE , *WEIGHT gain , *BODY composition of fish - Abstract
A study was undertaken to determine the dietary protein level for optimal growth performance and body composition of juvenile Senegalese sole. Five experimental extruded diets were formulated to contain increasing levels of protein [430, 480, 530, 570 and 600 g kg−1 dry matter (DM)] and a constant lipid level, ranging from 100 to 130 g kg−1 DM. Triplicate groups of 35 sole (initial body weight: 11.9 ± 0.5 g) were grown over 84 days in 60-L tanks supplied with recirculated seawater. Fish were fed by means of automatic feeders in eight meals per day. At the start and end of the trial, whole-body samples were withdrawn for proximate composition analysis. At the end of 84 days of experimental feeding, daily weight gain and specific growth rate in fish fed diets P43 and P48 were significantly lower than those found in fish fed higher protein level diets (P53, P57 and P59). Similarly, feed efficiency was also significantly lower in fish fed diet P43 than in fish fed all other dietary treatments. Sole juveniles fed lower protein level diets (P43 and P48) showed a significantly lower protein content than fish fed the higher dietary protein level treatments (P53, P57 and P60). Changes within the tested dietary protein levels did not affect significantly protein productive value or total nitrogen (N) losses in fish. However, daily N gain was significantly higher ( P < 0.05) in fish fed diets P53 and P60 than in fish fed the lowest protein level diet (P43). Data from the present study indicate that diets for juvenile Senegalese sole should include at least 53% crude protein to maintain a good overall growth performance. Based on a second-order polynomial regression model, the daily crude protein requirement for maximum whole-body N gain as estimated here for Senegalese sole juveniles was 6.43 g kg−1 body weight day−1 which corresponds to a value of 1.03 g N intake kg−1 body weight day−1. If the present data are expressed on a dietary crude protein concentration basis, the allowance for maximum protein accretion (N gain) would be met by a diet containing a crude protein level of 600 g kg−1. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
3. Digestion and absorption of a pure triacylglycerol and a free fatty acid by Clupea harengus L. larvae.
- Author
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Morais, S., Rojas-Garcia, C. R., Conceição, L. E. C., and Rønnestad, I.
- Subjects
FISH metabolism ,ATLANTIC herring ,FISH digestive organs ,FATTY acids ,FISH feeds ,DIGESTION - Abstract
The digestion, absorption and post absorptive metabolism of a radiolabelled triacylglycerol (TAG; triolein) and a free fatty acid (FFA; oleic acid), delivered by tube feeding, was studied in herring Clupea harengus larvae, using metabolic chambers and video analysis. In general, a large amount of the delivered lipid was evacuated. Most of the evacuation occurred between 2 and 6 h after tube feeding although a group of larvae responded by rapidly evacuating the lipid (>50% before 2 h). The volume of the tube-fed lipid affected its utilization. A small volume of triolein (9·2 nl, representing c. 6% of gut filling capacity) resulted in a lower proportion of fast evacuating larvae and improved utilization (lower evacuation and higher absorption: body incorporation and catabolism) compared with 50·6 nl ( c. 17% of gut filling capacity). Increases in the volume of tube fed triolein enhanced only marginally label absorption and led to a steep rise in evacuation. At a comparable high volume (50·6 nl), oleic acid, which does not require digestion, was better absorbed and less evacuated than triolein. The video observation of the lipid digestive process revealed a considerable gut contractile activity that appeared effective in processing the tube fed lipid. Also, the gut wall seemed very sensitive to physical pressure. Signs of chemical degradation during lipid digestion were also noted. The metabolic studies, together with video image analysis, suggested that the limiting step for the utilization of high dietary lipid levels may have been the lipid absorption into the enterocytes and transport into the body, rather than lipid digestion. The results support the notion that the rate of lipid digestion and absorption in fishes is slower than that of mammals. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
4. Ontogeny of the digestive capacity of Senegalese sole (Solea senegalensis), with respect to digestion, absorption and metabolism of amino acids from Artemia.
- Author
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Morais, S., Lacuisse, M., Conceição, L. E. C., Dinis, M. T., and Rønnestad, I.
- Subjects
LARVAE ,AMINO acids ,ARTEMIA ,ORGANIC acids ,ARTEMIIDAE ,PHYSICAL & theoretical chemistry ,NUTRITION - Abstract
Ontogenetic changes in the capacity of Senegalese sole (Solea senegalensis Kaup, 1858) larvae to digest and metabolise Artemia protein and amino acids (AA) were studied using 12, 22 and 35 days after hatching (DAH) larvae that were fed Artemia metanauplii radiolabelled with a [U-
14 C] protein hydrolysate. About 82% and 18% of the label was incorporated into the Artemia trichloroacetic acid (TCA) precipitate (mostly protein) and soluble (mainly free AA) fractions, respectively. The digestibility of Artemia was high at all tested ages, with label absorption varying between 77% and 83% at 24 h after feeding (HAF). A rapid digestion, absorption and catabolism of Artemia AA were noted, with most of the absorption into the body occurring during the first 3 HAF. Traces of label were already found in the metabolic-CO2 trap at 1 HAF. Furthermore, label was largely and almost immediately incorporated into the TCA precipitate fraction (mostly protein) of gut and body tissues. Slight differences were noted in diet utilization between larvae at different ages. At 12 DAH larvae had a lower catabolism and evacuation of the label, as well as the highest accumulation in the gut. However, except for the amount of catabolised label, the results were not significantly different from those for 35 DAH larvae. Moreover, no significant differences were found in the amount of label incorporated into the body, although it seemed to be higher in 12 DAH larvae. Taken together, these results reveal a higher absorption and a significantly higher retention of the absorbed label in 12 DAH larvae. In addition, 12 DAH larvae appeared to have a slower absorption of the label, which, in continuously feeding larvae, might result in overall lower food absorption efficiency. Therefore, it seems that young larvae have the ability to compensate for a possible lower digestive capacity with higher body retention of absorbed AA. This study confirms that sole larvae, even young stages, have a high capacity for digesting live preys. [ABSTRACT FROM AUTHOR]- Published
- 2004
- Full Text
- View/download PDF
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