1. Nested epistasis enhancer networks for robust genome regulation.
- Author
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Xueqiu Lin, Yanxia Liu, Shuai Liu, Xiang Zhu, Lingling Wu, Yanyu Zhu, Dehua Zhao, Xiaoshu Xu, Chemparathy, Augustine, Haifeng Wang, Yaqiang Cao, Muneaki Nakamura, Noordermeer, Jasprina N., Russa, Marie La, Wing Hung Wong, Keji Zhao, and Qi, Lei S.
- Subjects
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EPISTASIS (Genetics) , *GENOMES , *GENE expression , *GENE enhancers , *NUCLEIC acid regulatory sequences - Abstract
Mammalian genomes have multiple enhancers spanning an ultralong distance (>megabases) to modulate important genes, but it is unclear how these enhancers coordinate to achieve this task. We combine multiplexed CRISPRi screening with machine learning to define quantitative enhancer-enhancer interactions. We find that the ultralong distance enhancer network has a nested multilayer architecture that confers functional robustness of gene expression. Experimental characterization reveals that enhancer epistasis is maintained by three-dimensional chromosomal interactions and BRD4 condensation. Machine learning prediction of synergistic enhancers provides an effective strategy to identify noncoding variant pairs associated with pathogenic genes in diseases beyond genome-wide association studies analysis. Our work unveils nested epistasis enhancer networks, which can better explain enhancer functions within cells and in diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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