1. One Week of CDAHFD Induces Steatohepatitis and Mitochondrial Dysfunction with Oxidative Stress in Liver.
- Author
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Sugasawa T, Ono S, Yonamine M, Fujita SI, Matsumoto Y, Aoki K, Nakano T, Tamai S, Yoshida Y, Kawakami Y, and Takekoshi K
- Subjects
- Animals, Biomarkers, Disease Models, Animal, Disease Susceptibility, Gluconeogenesis, Inflammation Mediators metabolism, Lipid Droplets metabolism, Lipid Metabolism, Lipogenesis, Liver Cirrhosis etiology, Liver Cirrhosis metabolism, Liver Cirrhosis pathology, Mice, Non-alcoholic Fatty Liver Disease pathology, Phenotype, Choline Deficiency complications, Diet, High-Fat adverse effects, Mitochondria drug effects, Mitochondria metabolism, Non-alcoholic Fatty Liver Disease etiology, Non-alcoholic Fatty Liver Disease metabolism, Oxidative Stress
- Abstract
The prevalence of nonalcoholic fatty liver disease (NAFLD) has been rapidly increasing worldwide. A choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) has been used to create a mouse model of nonalcoholic steatohepatitis (NASH). There are some reports on the effects on mice of being fed a CDAHFD for long periods of 1 to 3 months. However, the effect of this diet over a short period is unknown. Therefore, we examined the effect of 1-week CDAHFD feeding on the mouse liver. Feeding a CDAHFD diet for only 1-week induced lipid droplet deposition in the liver with increasing activity of liver-derived enzymes in the plasma. On the other hand, it did not induce fibrosis or cirrhosis. Additionally, it was demonstrated that CDAHFD significantly impaired mitochondrial respiration with severe oxidative stress to the liver, which is associated with a decreasing mitochondrial DNA copy number and complex proteins. In the gene expression analysis of the liver, inflammatory and oxidative stress markers were significantly increased by CDAHFD. These results demonstrated that 1 week of feeding CDAHFD to mice induces steatohepatitis with mitochondrial dysfunction and severe oxidative stress, without fibrosis, which can partially mimic the early stage of NASH in humans.
- Published
- 2021
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