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Aerobic organisms including the gut microbiota have an essential antioxidant status, as a result of which these bacteria protect organisms from various pathologies and diseases. The goal of the given investigation is (1) the isolation and purification of the isoforms of endogenous О2−-producing associate from gastrointestinal bacteria (Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium bifidum); (2) determination of the effective concentrations of exogenous О2− produced by a complex of NADPH-containing protein component and Fe(III) (NPC-Fe(III)) from raspberries on the growth of the gastrointestinal bacteria in a nutrient medium in vitro. Ion-exchange chromatography on cellulose DE-52 and gel filtration on Sephadex G-100 at the pH of 9.5 was used to isolate and purify the NLP-Nox isoforms. Specific maximal optical absorption spectra of the Nox isoforms were observed in a weakly opalescent aqueous solution of the NLP-Nox isoforms. The specific contents of these NLP-Nox isoforms, as well as their composition, the stationary concentration of produced О2−, and the mechanism of О2− production were determined. The stimulating effect on the growth of these gastrointestinal bacteria in the nutrient medium of MRS broth and MRS agar in vitro under the influence of О2−, as a product of a new thermostable and acid-stable complex NPC-Fe(III) was determined. The NPC-Fe(III) complex, from raspberries was determined as well. Thus, for the first time, the isolation and purification of О2−- producing thermostable NADPH-containing lipoprotein–NADPH oxidase (NLP-Nox) associate from gastrointestinal bacteria membranes (continuously producing О2− under the aerobic conditions), and the stimulation of these bacteria growth by О2− formed by the complex from raspberries were demonstrated. The О2−-producing associate NLP-Nox was isolated and purified from the gut microbiota. NLP-Nox associate produces О2− by using a protein-bound non-free NADPH as a substrate. The NPC-Fe(III) isolated from raspberries generates О2−. The effective quantities of О2− promotes the growth and development of bacteria. [ABSTRACT FROM AUTHOR]