1. Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression.
- Author
-
Spiegel H, Schillberg S, and Nölke G
- Subjects
- Plant Leaves metabolism, Plants, Genetically Modified metabolism, Recombinant Proteins metabolism, Agrobacterium tumefaciens genetics, Agrobacterium tumefaciens metabolism, Nicotiana metabolism
- Abstract
The agroinfiltration of plant tissue is a robust method that allows the rapid and transient expression of recombinant proteins. Using wild-type plants as biomass, agroinfiltration exploits the ability of plants to synthesize even complex multimeric proteins that require oxidative folding and/or post-translational modifications, while avoiding the expensive and time-consuming creation of stably transformed plant lines. Here we describe a generic method for the transient expression of recombinant proteins in Nicotiana benthamiana at the small to medium laboratory scale, including appropriate binary vectors, the design and cloning of expression constructs, the transformation, selection, and cultivation of recombinant Agrobacterium tumefaciens, the infiltration of plants using a syringe or vacuum device, and finally the extraction of recombinant proteins from plant tissues., (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2022
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