Search

Your search keyword '"Tau H. Lee"' showing total 2 results
Start Over Author "Tau H. Lee" Topic new zealand
2 results on '"Tau H. Lee"'

1. Whole genome analysis of cephalosporin-resistant Escherichia coli from bloodstream infections in Australia, New Zealand and Singapore: high prevalence of CMY-2 producers and ST131 carrying blaCTX-M-15 and blaCTX-M-27

Author

Alexander M. Wailan, Mark A. Schembri, Leah W. Roberts, Amy Crowe, Roland Jureen, Merino Trial Investigators, Scott A. Beatson, Nouri L. Ben Zakour, Hasan Bhally, Hosam M. Zowawi, Ezlyn Izharuddin, Mo Yin, David L. Paterson, David Looke, Paul A. Tambyah, Naomi Runnegar, David C. Lye, Patrick N A Harris, Tau H. Lee, and Benjamin A. Rogers

Subjects
0301 basic medicine, Microbiology (medical), Male, medicine.drug_class, 030106 microbiology, Cephalosporin, Bacteremia, Drug resistance, Biology, medicine.disease_cause, beta-Lactamases, Microbiology, 03 medical and health sciences, Drug Resistance, Bacterial, medicine, Escherichia coli, Prevalence, Humans, Pharmacology (medical), Blood culture, Prospective Studies, Clade, Etest, Escherichia coli Infections, Phylogeny, Aged, Randomized Controlled Trials as Topic, Pharmacology, Aged, 80 and over, Singapore, medicine.diagnostic_test, Whole Genome Sequencing, Australia, Middle Aged, Bacterial Typing Techniques, Cephalosporins, Ciprofloxacin, Infectious Diseases, Multilocus sequence typing, Female, Genome, Bacterial, medicine.drug, Multilocus Sequence Typing, New Zealand
Abstract

Objectives To characterize MDR Escherichia coli from bloodstream infections (BSIs) in Australia, New Zealand and Singapore. Methods We collected third-generation cephalosporin-resistant (3GC-R) E. coli from blood cultures in patients enrolled in a randomized controlled trial from February 2014 to August 2015. WGS was used to characterize antibiotic resistance genes, MLST, plasmids and phylogenetic relationships. Antibiotic susceptibility was determined using disc diffusion and Etest. Results A total of 70 3GC-R E. coli were included, of which the majority were ST131 (61.4%). BSI was most frequently from a urinary source (69.6%), community associated (62.9%) and in older patients (median age 71 years). The median Pitt score was 1 and ICU admission was infrequent (3.1%). ST131 possessed more acquired resistance genes than non-ST131 (P = 0.003). Clade C1/C2 ST131 predominated (30.2% and 53.5% of ST131, respectively) and these were all ciprofloxacin resistant. All clade A ST131 (n = 6) were community associated. The predominant ESBL types were blaCTX-M (80.0%) and were strongly associated with ST131 (95% carried blaCTX-M), with the majority blaCTX-M-15. Clade C1 was associated with blaCTX-M-14 and blaCTX-M-27, whereas blaCTX-M-15 predominated in clade C2. Plasmid-mediated AmpC genes (mainly blaCMY-2) were frequent (17.1%) but were more common in non-ST131 (P

Published
2017

2. Whole genome analysis of cephalosporin-resistant Escherichia coli from bloodstream infections in Australia, New Zealand and Singapore: high prevalence of CMY-2 producers and ST131 carrying blaCTX-M-15 and blaCTX-M-27.

Author

Harris, Patrick N. A., Ben Zakour, Nouri L., Roberts, Leah W., Wailan, Alexander M., Zowawi, Hosam M., Tambyah, Paul A., Lye, David C., Jureen, Roland, Tau H. Lee, Mo Yin, Izharuddin, Ezlyn, Looke, David, Runnegar, Naomi, Rogers, Benjamin, Bhally, Hasan, Crowe, Amy, Schembri, Mark A., Beatson, Scott A., Paterson, David L., and Lee, Tau H

Subjects
GENOMES, CEPHALOSPORINS, ESCHERICHIA coli, DISEASE prevalence, RANDOMIZED controlled trials, BACTEREMIA, BACTERIOPHAGE typing, CLINICAL trials, DRUG resistance in microorganisms, ESCHERICHIA coli diseases, BIOLOGICAL evolution, HYDROLASES, LONGITUDINAL method, SEQUENCE analysis, PHARMACODYNAMICS
Abstract

Objectives: To characterize MDR Escherichia coli from bloodstream infections (BSIs) in Australia, New Zealand and Singapore.Methods: We collected third-generation cephalosporin-resistant (3GC-R) E. coli from blood cultures in patients enrolled in a randomized controlled trial from February 2014 to August 2015. WGS was used to characterize antibiotic resistance genes, MLST, plasmids and phylogenetic relationships. Antibiotic susceptibility was determined using disc diffusion and Etest.Results: A total of 70 3GC-R E. coli were included, of which the majority were ST131 (61.4%). BSI was most frequently from a urinary source (69.6%), community associated (62.9%) and in older patients (median age 71 years). The median Pitt score was 1 and ICU admission was infrequent (3.1%). ST131 possessed more acquired resistance genes than non-ST131 (P = 0.003). Clade C1/C2 ST131 predominated (30.2% and 53.5% of ST131, respectively) and these were all ciprofloxacin resistant. All clade A ST131 (n = 6) were community associated. The predominant ESBL types were blaCTX-M (80.0%) and were strongly associated with ST131 (95% carried blaCTX-M), with the majority blaCTX-M-15. Clade C1 was associated with blaCTX-M-14 and blaCTX-M-27, whereas blaCTX-M-15 predominated in clade C2. Plasmid-mediated AmpC genes (mainly blaCMY-2) were frequent (17.1%) but were more common in non-ST131 (P < 0.001) isolates from Singapore and Brisbane. Two strains carried both blaCMY-2 and blaCTX-M. The majority of plasmid replicon types were IncF.Conclusions: In a prospective collection of 3GC-R E. coli causing BSI, community-associated Clade C1/C2 ST131 predominate in association with blaCTX-M ESBLs, although a significant proportion of non-ST131 strains carried blaCMY-2. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results