1. Phospholipase A 2 Inhibitor from Crotalus durissus terrificus rattlesnake: Effects on human peripheral blood mononuclear cells and human neutrophils cells.
- Author
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Xavier CV, da S Setúbal S, Lacouth-Silva F, Pontes AS, Nery NM, de Castro OB, Fernandes CFC, Soares AM, Fortes-Dias CL, and Zuliani JP
- Subjects
- Animals, Cell Proliferation drug effects, Cell Survival drug effects, Chemotaxis drug effects, Cytokines metabolism, Cytosol enzymology, Humans, Interleukin-10 metabolism, Interleukin-2 metabolism, Interleukin-8 metabolism, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear metabolism, Leukotriene B4 biosynthesis, Neutrophils cytology, Neutrophils metabolism, Peroxidase metabolism, Superoxides metabolism, Tumor Necrosis Factor-alpha metabolism, Crotalus, Leukocytes, Mononuclear drug effects, Neutrophils drug effects, Phospholipase A2 Inhibitors pharmacology
- Abstract
Crotalus Neutralizing Factor (CNF) is an inhibitor of phospholipase A
2 (PLA2 ), present in the blood plasma of Crotalus durissus terrificus snake. This inhibitor neutralizes the lethal and enzymatic activity of crotoxin, the main neurotoxin from this venom. In this study, we investigated the effects of CNF on the functionality of human peripheral blood mononuclear cells (PBMCs) and human neutrophils. The following parameters were evaluated: viability and proliferation, chemotaxis, cytokines and LTB4 production, cytosolic PLA2 s activity, myeloperoxidase (MPO) and superoxide anion (O2 ) production. CNF showed no toxicity on PBMCs or neutrophils, and acts by stimulating the release of TNF-α and LTB- ) production. CNF showed no toxicity on PBMCs or neutrophils, and acts by stimulating the release of TNF-α and LTB4 release. In neutrophils, CNF induces chemotaxis but does not induce the release of both MPO and O2 - release. In neutrophils, CNF induces chemotaxis but does not induce the release of both MPO and O2 production, and on neutrophils, by stimulating chemotaxis and LTB- . However, it induces LTB4 and IL-8 production. These data show the influence of CNF on PBMCs' function by inducing TNF-α and LTB4 production, and on neutrophils, by stimulating chemotaxis and LTB4 production, via cytosolic PLA2 activity, and IL-8 release. The inflammatory profile produced by CNF is shown for the first time. Our present results suggest that CNF has a role in activation of leukocytes and exert proinflammatory effects on these cell., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2017
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