1. [Mechanism of Ca2+ on the hyperthermia-induced apoptosis of rat hippocampal neurons in vitro].
- Author
-
Chen GZ, Luo BD, Chen XH, Zhao QP, Zou F, and Li TL
- Subjects
- Animals, Animals, Newborn, Cells, Cultured, Female, Hippocampus drug effects, Male, Neurons drug effects, Rats, Rats, Sprague-Dawley, Temperature, Apoptosis drug effects, Calcium metabolism, Calcium Channel Blockers pharmacology, Dantrolene pharmacology, Hippocampus cytology, Neurons metabolism
- Abstract
Objective: To study the mechanism of Ca(2+) on the apoptosis induced by hyperthermia in neonate rat hippocampal neurons to provide the applicative evidence of dantrolene for preventing brain injuries., Methods: Dantrolene, Ca(2+) specific blocking agent, was used in the hyperthermia-induced apoptosis of primary hippocampal neurons in vitro to observe its effect on the apoptosis, fluorescent intensity, and dynamic change of Ca(2+) by flowcytometry and laser confocal microscopy., Results: The rate of apoptosis was decreased significantly after hyperthermia treatment by dantrolene sodium. The intracellular Ca(2+) fluorescent intensity in 42 degrees C treatment group (107.35 +/- 6.0) was significantly lower than that in control group (159.12 +/- 33.8). The concentration of Ca(2+) began to decrease 20 approximately 25 s after adding dantrolene sodium, and reached the lowest level about 50 s later, and then kept lower than the basal level., Conclusion: Dantrolene sodium has an important protective effect on hippocampal neurons apoptosis induced by hyperthermia and may have some applicative value of preventing heat-induced brain injury.
- Published
- 2005