1. NB-02 Protects Neurons and Astrocytes from Oligomeric Amyloid-β-Mediated Damage.
- Author
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Miller MR, Lariviere L, Pagnier GJ, Aygar S, Wieckiewicz N, Maesako M, Bacskai BJ, and Kastanenka KV
- Subjects
- Animals, Mice, Cells, Cultured, Calcium metabolism, Peptide Fragments toxicity, Peptide Fragments pharmacology, Humans, Astrocytes drug effects, Astrocytes metabolism, Amyloid beta-Peptides metabolism, Amyloid beta-Peptides toxicity, Neurons drug effects, Neurons metabolism, Neurons pathology, Coculture Techniques, Neuroprotective Agents pharmacology
- Abstract
Alzheimer's disease (AD) is a progressive neurodegenerative disease with limited therapeutic strategies. NB-02 is a novel botanical drug that has shown promise as a protective and therapeutic treatment for AD in an APP/PS1 preclinical mouse model. In this paper, we investigate the underlying mechanisms by which NB-02 provides these therapeutic advantages using in vitro neuron-astrocyte co-cultures. Pretreatment with NB-02 prevented pathological calcium elevations in neurons and astrocytes after application of toxic soluble amyloid-β (Aβ) oligomers. NB-02 also prevented cell death associated with the addition of soluble Aβ oligomers suggesting NB-02 is effective at protecting both neurons and astrocytes from Aβ-mediated damage.
- Published
- 2024
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