1. The intrinsic electrophysiological properties of neurons derived from mouse embryonic stem cells overexpressing neurogenin-1.
- Author
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Tong M, Hernandez JL, Purcell EK, Altschuler RA, and Duncan RK
- Subjects
- Action Potentials drug effects, Animals, Basic Helix-Loop-Helix Transcription Factors genetics, Calcium Channels drug effects, Calcium Channels physiology, Cell Line, Membrane Potentials drug effects, Membrane Potentials physiology, Mice, Nerve Tissue Proteins genetics, Potassium Channels, Voltage-Gated drug effects, Potassium Channels, Voltage-Gated physiology, Sodium Channels drug effects, Sodium Channels physiology, Tetrodotoxin pharmacology, Action Potentials physiology, Basic Helix-Loop-Helix Transcription Factors biosynthesis, Embryonic Stem Cells physiology, Nerve Tissue Proteins biosynthesis, Neurogenesis, Neurons physiology
- Abstract
A mouse embryonic stem (ES) cell line containing an inducible transgene for the proneural gene Neurog1 has been used to generate glutamatergic neurons at a high efficiency. The present study used in vitro electrophysiology to establish the timeline for acquiring a functional neuronal phenotype in Neurog1-induced cells exhibiting a neuronal morphology. TTX-sensitive action potentials could be evoked from over 80% of the cells after only 4.5 days in vitro (DIV). These cells uniformly showed rapidly adapting responses to current injection, firing one to three action potentials at the onset of the stimulus. In the absence of Neurog1, a limited number of ES cells adopted a neuronal morphology, but these cells displayed slow calcium depolarizations rather than sodium-based spikes. Voltage-gated Na(+), K(+), and Ca(2+) currents were present in nearly all induced cells as early as 4.5 DIV. The voltage-dependent properties of these currents changed little from 4 to 12 DIV with half-activation voltage varying by <10 mV for any current type throughout the culture period. This study demonstrates that forced expression of proneural genes can induce ES cells to quickly acquire a functional neuronal phenotype with mature electrophysiological properties. Transient overexpression of Neurog1 may be used in neural repair strategies that require the rapid induction of functional neurons from pluripotent stem cells.
- Published
- 2010
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