1. A Fluorescent Probe for Neural Stem/Progenitor Cells with High Differentiation Capability into Neurons.
- Author
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Kim B, Feng S, Yun SW, Leong C, Satapathy R, Wan SY, and Chang YT
- Subjects
- ATP Binding Cassette Transporter, Subfamily G, Member 2 antagonists & inhibitors, ATP Binding Cassette Transporter, Subfamily G, Member 2 genetics, ATP Binding Cassette Transporter, Subfamily G, Member 2 metabolism, Animals, Benzamides metabolism, Biomarkers metabolism, Brain cytology, Brain metabolism, Cell Differentiation, Cells, Cultured, Embryo, Mammalian cytology, Humans, Mice, Microscopy, Fluorescence, Neoplasm Proteins antagonists & inhibitors, Neoplasm Proteins genetics, Neoplasm Proteins metabolism, Neural Stem Cells chemistry, Neural Stem Cells cytology, Neurons cytology, Propionates chemistry, Propionates metabolism, Quinolines chemistry, Quinolines metabolism, RNA Interference, RNA, Small Interfering metabolism, Xanthenes metabolism, Benzamides chemistry, Fluorescent Dyes chemistry, Neural Stem Cells metabolism, Neurons metabolism, Xanthenes chemistry
- Abstract
Selection of a specific neural stem/progenitor cells (NSPCs) has attracted broad attention in regenerative medicine for neurological disorders. Here, we report a fluorescent probe, CDg13, and its application for isolating strong neurogenic NSPCs. In comparison to the NSPCs isolated by other biomarkers, CDg13-stained NSPCs showed higher capability to differentiate into neurons. Target identification revealed that the fluorescence intensity of the probe within cells is inversely proportional to the expression levels of mouse and human Abcg2 transporters. These findings suggest that low Abcg2 expression is a biomarker for neurogenic NSPCs in mouse brain. Furthermore, CDg13 can be used to isolate Abcg2
low cells from heterogeneous cell populations., (© 2016 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)- Published
- 2016
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