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The song of a male zebra finch is a stereotyped motor sequence whose tempo varies with social context - whether or not the song is directed at a female bird - as well as with the time of day. The neural mechanisms underlying these changes in tempo are unknown. Here we show that brain temperature recorded in freely behaving male finches exhibits a global increase in response to the presentation of a female bird. This increase strongly correlates with, and largely explains, the faster tempo of songs directed at a female compared to songs produced in social isolation. Furthermore, we find that the observed diurnal variations in song tempo are also explained by natural variations in brain temperature. Our findings suggest that brain temperature is an important variable that can influence the dynamics of activity in neural circuits, as well as the temporal features of behaviors that some of these circuits generate. [ABSTRACT FROM AUTHOR]

Accurate timing is a critical aspect of motor control, yet the temporal structure of many mature behaviors emerges during learning from highly variable exploratory actions. How does a developing brain acquire the precise control of timing in behavioral sequence ? To investigate the development of timing, we analyzed the songs of young juvenile zebra finches. These highly variable vocalizations, akin to human babbling, gradually develop into temporally stereotyped adult songs. We find that the durations of syllables and silences in juvenile singing are formed by a mixture of two distinct modes of timing: a random mode producing broadly distributed durations early in development, and a stereotyped mode underlying the gradual emergence of stereotyped durations. Using lesions, inactivations, and localized brain cooling, we investigated the roles of neural dynamics within two premotor cortical areas in the production of these temporal modes. We find that LMAN (lateral magnocellular nucleus of the nidopallium) is required specifically for the generation of the random mode of timing and that mild cooling ofLMAN causes an increase in the durations produced by this mode. On the contrary, HVC (used as a proper name) is required specifically for producing the stereotyped mode of timing, and its cooling causes a slowing of all stereotyped components. These results show that two neural pathways contribute to the timing of juvenile songs and suggest an interesting organization in the forebrain, whereby different brain areas are specialized for the production of distinct forms of neural dynamic . [ABSTRACT FROM AUTHOR]

Abstract: Traditional lesion or inactivation methods are useful for determining if a given brain area is involved in the generation of a behavior, but not for determining if circuit dynamics in that area control the timing of the behavior. In contrast, localized mild cooling or heating of a brain area alters the speed of neuronal and circuit dynamics and can reveal the role of that area in the control of timing. It has been shown that miniaturized solid-state heat pumps based on the Peltier effect can be useful for analyzing brain dynamics in small freely behaving animals (). Here we present a theoretical analysis of these devices and a procedure for optimizing their design. We describe the construction and implementation of one device for cooling surface brain areas, such as cortex, and another device for cooling deep brain regions. We also present measurements of the magnitude and localization of the brain temperature changes produced by these two devices. [Copyright &y& Elsevier]

Young animals engage in variable exploratory behaviors essential for the development of neural circuitry and adult motor control, yet the neural basis of these behaviors is largely unknown. Juvenile songbirds produce subsong--a succession of primitive vocalizations akin to human babbling. We found that subsong production in zebra finches does not require HVC (high vocal center), a key premotor area for singing in adult birds, but does require LMAN (lateral magnocellular nucleus of the nidopallium), a forebrain nucleus involved in learning but not in adult singing. During babbling, neurons in LMAN exhibited premotor correlations to vocal output on a fast time scale. Thus, juvenile singing is driven by a circuit distinct from that which produces the adult behavior--a separation possibly general to other developing motor systems. [ABSTRACT FROM AUTHOR]

How can neural activity propagate through cortical networks built with weak, stochastic synapses? We find precise repetitions of spontaneous patterns of synaptic inputs in neocortical neurons in vivo and in vitro. These patterns repeat after minutes, maintaining millisecond accuracy. Calcium imaging of slices reveals reactivation of sequences of cells during the occurrence of repeated intracellular synaptic patterns. The spontaneous activity drifts with time, engaging different cells. Sequences of active neurons have distinct spatial structures and are repeated in the same order over tens of seconds, revealing modular temporal dynamics. Higher order sequences are replayed with compressed timing. [ABSTRACT FROM AUTHOR]