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The cerebellar cortex of PN15 wt and Npc1 nmf164 mice diplays similar densities of Bergmann glia, Purkinje cells and basket/stellate interneurons. The number of Bergmann glia, PCs and basket/stellate interneurons was determined in cerebellar sections of PN15 wt and Npc1 nmf164 mice stained with hematoxylin/eosin Y (right panel; asterisks: migrating GNs; arrows: basket/stellate interneurons; arrowheads: Bergmann glia) or processed for immunostaining with anti-parvalbumin antibody (left panel) to identify GABA-ergic neurons/interneurons. Scale bar: 50 μm. Histograms represent cell densities (mean ± SEM of all sections examined; N = 3 mice/genotype; 3–4 sections/mouse) determined in 0.04 mm2 regions randomly selected in each microscopic field of anterior (I-V) and posterior (VI-X) lobules of wt and Npc1 nmf164 mouse cerebella, stained with hematoxylin/eosin Y (right) or anti-parvalbumin antibody (left). Since any significant difference was found between counts of anterior and posterior lobules, values were averaged. Comparisons were performed by unpaired two-tailed Student’s t test. (PDF 3404 kb)

A Western blot analysis of GFAP protein expression in cerebella of PN11 wt and Npc1 nmf164 mice. B Histograms indicate the abundance (mean ± SEM) of each isoform determined by densitometry of protein bands obtained in at least 3 independent experiments taking β-actin as internal reference. (PDF 95 kb)

The cerebellar cortex of PN15 wt and Npc1 nmf164 mice diplays similar densities of Bergmann glia, Purkinje cells and basket/stellate interneurons. The number of Bergmann glia, PCs and basket/stellate interneurons was determined in cerebellar sections of PN15 wt and Npc1 nmf164 mice stained with hematoxylin/eosin Y (right panel; asterisks: migrating GNs; arrows: basket/stellate interneurons; arrowheads: Bergmann glia) or processed for immunostaining with anti-parvalbumin antibody (left panel) to identify GABA-ergic neurons/interneurons. Scale bar: 50 μm. Histograms represent cell densities (mean ± SEM of all sections examined; N = 3 mice/genotype; 3–4 sections/mouse) determined in 0.04 mm2 regions randomly selected in each microscopic field of anterior (I-V) and posterior (VI-X) lobules of wt and Npc1 nmf164 mouse cerebella, stained with hematoxylin/eosin Y (right) or anti-parvalbumin antibody (left). Since any significant difference was found between counts of anterior and posterior lobules, values were averaged. Comparisons were performed by unpaired two-tailed Student’s t test. (PDF 3404 kb)

A Western blot analysis of GFAP protein expression in cerebella of PN11 wt and Npc1 nmf164 mice. B Histograms indicate the abundance (mean ± SEM) of each isoform determined by densitometry of protein bands obtained in at least 3 independent experiments taking β-actin as internal reference. (PDF 95 kb)