78 results on '"Sternberg, Paul W."'
Search Results
2. Pristionchus pacificus, a Nematode with Only Three Juvenile Stages, Displays Major Heterochronic Changes Relative to Caenorhabditis elegans
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Felix, Marie-Anne, Hill, Russell J., Schwarz, Heinz, Sternberg, Paul W., Sudhaus, Walter, and Sommer, Ralf J.
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- 1999
3. Communication between oocytes and somatic cells regulates volatile pheromone production in Caenorhabditis elegans
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Leighton, Daniel H. W., Choe, Andrea, Wu, Shannon Y, and Sternberg, Paul W.
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- 2014
4. Sex-specific mating pheromones in the nematode Panagrellus redivivus
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Choe, Andrea, Chuman, Tatsuji, von Reuss, Stephan H., Dossey, Aaron T., Yim, Joshua J., Ajredini, Ramadan, Kolawa, Adam A., Kaplan, Fatma, Alborn, Hans T., Teal, Peter E. A., Schroeder, Frank C., Sternberg, Paul W., and Edison, Arthur S.
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- 2012
5. Nematode-Bacterium Symbioses—Cooperation and Conflict Revealed in the “Omics” Age
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MURFIN, KRISTEN E., DILLMAN, ADLER R., FOSTER, JEREMY M., BULGHERESI, SILVIA, SLATKO, BARTON E., STERNBERG, PAUL W., and GOODRICH-BLAIR, HEIDI
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- 2012
6. Receptor-type guanylate cyclase is required for carbon dioxide sensation by Caenorhabditis elegans
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Hallem, Elissa A., Spencer, W. Clay, McWhirter, Rebecca D., Zeller, Georg, Henz, Stefan R., Rätsch, Gunnar, Miller, David M., Horvitz, H. Robert, Sternberg, Paul W., and Ringstad, Niels
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- 2011
7. Acute Carbon Dioxide Avoidance in Caenorhabditis elegans
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Hallem, Elissa A. and Sternberg, Paul W.
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- 2008
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8. Expressed Sequence Tag Analysis of Gene Representation in Insect Parasitic Nematode Heterorhabditis bacteriophora
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Bai, Xiaodong, Grewal, Parwinder S., Hogenhout, Saskia A., Adams, Byron J., Ciche, Todd A., Gaugler, Randy, and Sternberg, Paul W.
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- 2007
9. Changes of Induction and Competence During the Evolution of Vulva Development in Nematodes
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Sommer, Ralf J. and Sternberg, Paul W.
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- 1994
10. Possible stochastic sex determination in Bursaphelenchus nematodes.
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Shinya, Ryoji, Sun, Simo, Dayi, Mehmet, Tsai, Isheng Jason, Miyama, Atsushi, Chen, Anthony Fu, Hasegawa, Koichi, Antoshechkin, Igor, Kikuchi, Taisei, and Sternberg, Paul W.
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SEX determination ,GENETIC sex determination ,ENVIRONMENTAL sex determination ,CAENORHABDITIS elegans ,BURSAPHELENCHUS ,X chromosome ,NEMATODES - Abstract
Sex determination mechanisms evolve surprisingly rapidly, yet little is known in the large nematode phylum other than for Caenorhabditis elegans, which relies on chromosomal XX-XO sex determination and a dosage compensation mechanism. Here we analyze by sex-specific genome sequencing and genetic analysis sex determination in two fungal feeding/plant-parasitic Bursaphelenchus nematodes and find that their sex differentiation is more likely triggered by random, epigenetic regulation than by more well-known mechanisms of chromosomal or environmental sex determination. There is no detectable difference in male and female chromosomes, nor any linkage to sexual phenotype. Moreover, the protein sets of these nematodes lack genes involved in X chromosome dosage counting or compensation. By contrast, our genetic screen for sex differentiation mutants identifies a Bursaphelenchus ortholog of tra-1, the major output of the C. elegans sex determination cascade. Nematode sex determination pathways might have evolved by "bottom-up" accretion from the most downstream regulator, tra-1. In most species, sex is determined by genetic or environmental factors. Here, the authors present evidence that sex determination in Bursaphelenchus nematodes is instead likely to be regulated by a random, epigenetic mechanism. [ABSTRACT FROM AUTHOR]
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- 2022
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11. Using Transcriptomes as Mutant Phenotypes Reveals Functional Regions of a Mediator Subunit in Caenorhabditis elegans.
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Angeles-Albores, David and Sternberg, Paul W.
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ALLELES , *ANIMAL experimentation , *CONCEPTUAL structures , *GENE expression , *GENETIC mutation , *NEMATODES , *PHENOTYPES , *WNT proteins , *GENE expression profiling , *SIGNAL peptides - Abstract
Although transcriptomes have recently been used as phenotypes with which to perform epistasis analyses, they are not yet used to study intragenic function/structure relationships. We developed a theoretical framework to study allelic series using transcriptomic phenotypes. As a proof-of-concept, we apply our methods to an allelic series of dpy-22, a highly pleiotropic Caenorhabditis elegans gene orthologous to the human gene MED12, which encodes a subunit of the Mediator complex. Our methods identify functional units within dpy-22 that modulate Mediator activity upon various genetic programs, including the Wnt and Ras modules. [ABSTRACT FROM AUTHOR]
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- 2018
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12. FMRFamide-like peptides expand the behavioral repertoire of a densely connected nervous system.
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Lee, James Siho, Pei-Yin Shih, Schaedel, Oren N., Quintero-Cadena, Porfirio, Rogers, Alicia K., and Sternberg, Paul W.
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PEPTIDES ,RNA sequencing ,NEMATODES ,NUCLEOTIDE sequence ,CAENORHABDITIS elegans genetics - Abstract
Animals, including humans, can adapt to environmental stress through phenotypic plasticity. The free-living nematode Caenorhabditis elegans can adapt to harsh environments by undergoing a whole-animal change, involving exiting reproductive development and entering the stress-resistant dauer larval stage. The dauer is a dispersal stage with dauer-specific behaviors for finding and stowing onto carrier animals, but how dauers acquire these behaviors, despite having a physically limited nervous system of 302 neurons, is poorly understood. We compared dauer and reproductive development using whole-animal RNA sequencing at fine time points and at sufficient depth to measure transcriptional changes within single cells. We detected 8,042 genes differentially expressed during dauer and reproductive development and observed striking up-regulation of neuropeptide genes during dauer entry. We knocked down neuropeptide processing using sbt-1 mutants and demonstrate that neuropeptide signaling promotes the decision to enter dauer rather than reproductive development. We also demonstrate that during dauer neuropeptides modulate the dauerspecific nictation behavior (carrier animal-hitchhiking) and are necessary for switching from repulsion to CO
2 (a carrier animal cue) in nondauers to CO2 attraction in dauers. We tested individual neuropeptides using CRISPR knockouts and existing strains and demonstrate that the combined effects of flp-10 and flp-17 mimic the effects of sbt-1 on nictation and CO2 attraction. Through meta-analysis, we discovered similar up-regulation of neuropeptides in the dauer-like infective juveniles of diverse parasitic nematodes, suggesting the antiparasitic target potential of SBT-1. Our findings reveal that, under stress, increased neuropeptide signaling in C. elegans enhances their decision-making accuracy and expands their behavioral repertoire. [ABSTRACT FROM AUTHOR]- Published
- 2017
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13. CAP protein superfamily members in Toxocara canis.
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Stroehlein, Andreas J., Young, Neil D., Hall$1, Ross S., Korhonen, Pasi K., Hofmann, Andreas, Sternberg, Paul W., Jabbar, Abdul, and Gasser, Robin B.
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PROTEINS ,CYSTEINE ,ANTIGENS ,PATHOGENIC microorganisms ,IMMUNE response - Abstract
Background: Proteins of the cysteine-rich secretory proteins, antigen 5 and pathogenesis-related 1 (CAP) superfamily are recognized or proposed to play roles in parasite development and reproduction, and in modulating host immune attack and infection processes. However, little is known about these proteins for most parasites. Results: In the present study, we explored CAP proteins of Toxocara canis, a socioeconomically important zoonotic roundworm. To do this, we mined and curated transcriptomic and genomic data, predicted and curated full-length protein sequences (n = 28), conducted analyses of these data and studied the transcription of respective genes in different developmental stages of T. canis. In addition, based on information available for Caenorhabditis elegans, we inferred that selected genes (including lon-1, vap-1, vap-2, scl-1, scl-8 and scl-11 orthologs) of T. canis and their interaction partners likely play central roles in this parasite's development and/or reproduction via TGF-beta and/or insulin-like signaling pathways, or via host interactions. Conclusion: In conclusion, this study could provide a foundation to guide future studies of CAP proteins of T. canis and related parasites, and might assist in finding new interventions against diseases caused by these parasites. [ABSTRACT FROM AUTHOR]
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- 2016
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14. The Haemonchus contortus kinome - a resource for fundamental molecular investigations and drug discovery.
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Stroehlein, Andreas J., Young, Neil D., Korhonen, Pasi K., Jabbar, Abdul, Hofmann, Andreas, Sternberg, Paul W., and Gasser, Robin B.
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PROTEIN kinases ,EUKARYOTIC evolution ,NEMATODES ,HAEMONCHUS contortus ,DRUG resistance ,ANIMAL behavior - Abstract
Background: Protein kinases regulate a plethora of essential signalling and other biological pathways in all eukaryotic organisms, but very little is known about them in most parasitic nematodes. Methods: Here, we defined, for the first time, the entire complement of protein kinases (kinome) encoded in the barber's pole worm (Haemonchus contortus) through an integrated analysis of transcriptomic and genomic datasets using an advanced bioinformatic workflow. Results: We identified, curated and classified 432 kinases representing ten groups, 103 distinct families and 98 subfamilies. A comparison of the kinomes of H. contortus and Caenorhabditis elegans (a related, free-living nematode) revealed considerable variation in the numbers of casein kinases, tyrosine kinases and Ca
2+ / calmodulin-dependent protein kinases, which likely relate to differences in biology, habitat and life cycle between these worms. Moreover, a suite of kinase genes was selectively transcribed in particular developmental stages of H. contortus, indicating central roles in developmental and reproductive processes. In addition, using a ranking system, drug targets (n = 13) and associated small-molecule effectors (n = 1517) were inferred. Conclusions: The H. contortus kinome will provide a useful resource for fundamental investigations of kinases and signalling pathways in this nematode, and should assist future anthelmintic discovery efforts; this is particularly important, given current drug resistance problems in parasitic nematodes. [ABSTRACT FROM AUTHOR]- Published
- 2015
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15. A Toolkit of Engineered Recombinational Balancers in C. elegans.
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Schwartz, Hillel T. and Sternberg, Paul W.
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CAENORHABDITIS elegans , *CRISPRS , *GENETIC code , *CHROMOSOMES , *NEMATODES - Abstract
Dejima and colleagues report using CRISPR/Cas9 to generate a new collection of greatly improved balancer chromosomes in the standard laboratory nematode Caenorhabditis elegans , using methods previously reported by the same laboratory, expanding the set of C. elegans balancers to cover nearly 90% of coding genes. [ABSTRACT FROM AUTHOR]
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- 2018
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16. The cGMP Signaling Pathway Affects Feeding Behavior in the Necromenic Nematode Pristionchus pacificus.
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Kroetz, Silvina M., Srinivasan, Jagan, Yaghoobian, Jonathan, Sternberg, Paul W., and Hong, Ray L.
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BEETLES ,NEMATODES ,COMPARATIVE studies ,CAENORHABDITIS elegans ,PHARYNGEAL bursa ,METHODOLOGY - Abstract
Background: The genetic tractability and the species-specific association with beetles make the nematode Pristionchus pacificus an exciting emerging model organism for comparative studies in development and behavior. P. pacificus differs from Caenorhabditis elegans (a bacterial feeder) by its buccal teeth and the lack of pharyngeal grinders, but almost nothing is known about which genes coordinate P. pacificus feeding behaviors, such as pharyngeal pumping rate, locomotion, and fat storage. Methodology/Principal Findings: We analyzed P. pacificus pharyngeal pumping rate and locomotion behavior on and off food, as well as on different species of bacteria (Escherichia coli, Bacillus subtilis, and Caulobacter crescentus). We found that the cGMP-dependent protein kinase G (PKG) Ppa-EGL-4 in P. pacificus plays an important role in regulating the pumping rate, mouth form dimorphism, the duration of forward locomotion, and the amount of fat stored in intestine. In addition, Ppa-EGL-4 interacts with Ppa-OBI-1, a recently identified protein involved in chemosensation, to influence feeding and locomotion behavior. We also found that C. crescentus NA1000 increased pharyngeal pumping as well as fat storage in P. pacificus. Conclusions: The PKG EGL-4 has conserved functions in regulating feeding behavior in both C. elegans and P. pacificus nematodes. The Ppa-EGL-4 also has been co-opted during evolution to regulate P. pacificus mouth form dimorphism that indirectly affect pharyngeal pumping rate. Specifically, the lack of Ppa-EGL-4 function increases pharyngeal pumping, time spent in forward locomotion, and fat storage, in part as a result of higher food intake. Ppa-OBI-1 functions upstream or parallel to Ppa-EGL-4. The beetle-associated omnivorous P. pacificus respond differently to changes in food state and food quality compared to the exclusively bacteriovorous C. elegans. [ABSTRACT FROM AUTHOR]
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- 2012
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17. An Entomopathogenic Nematode by Any Other Name.
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Dillman, Adler R., Chaston, John M., Adams, Byron J., Ciche, Todd A., Goodrich-Blair, Heidi, Stock, S. Patricia, and Sternberg, Paul W.
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ENTOMOPATHOGENIC fungi ,INSECT nematodes ,BACTERIA classification ,NEMATODES ,ARTHROPODA - Abstract
The authors provide information on entomopathogenic nematodes (EPNs). They clarify the meaning of entomopathogenic in nematology, along with the nematode-arthropod associations. They argue that EPN must rapidly kill their hosts with the help of bacterial partners in order to create future generations. The authors also discuss the history, specialization and classification of EPNs.
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- 2012
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18. WormBase: Annotating many nematode genomes.
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Howe, Kevin, Davis, Paul, Paulini, Michael, Tuli, Mary Ann, Williams, Gary, Yook, Karen, Durbin, Richard, Kersey, Paul, and Sternberg, Paul W.
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SCIENTIFIC community ,NEMATODE genetics ,CAENORHABDITIS elegans genetics ,DATABASES ,NEMATODES - Abstract
WormBase (www.wormbase.org) has been serving the scientific community for over 11 years as the central repository for genomic and genetic information for the soil nematode Caenorhabditis elegans. The resource has evolved from its beginnings as a database housing the genomic sequence and genetic and physical maps of a single species, and now represents the breadth and diversity of nematode research, currently serving genome sequence and annotation for around 20 nematodes. In this article, we focus on WormBase's role of genome sequence annotation, describing how we annotate and integrate data from a growing collection of nematode species and strains. We also review our approaches to sequence curation, and discuss the impact on annotation quality of large functional genomics projects such as modENCODE. [ABSTRACT FROM AUTHOR]
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- 2012
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19. A comparison of experience-dependent locomotory behaviors and biogenic amine neurons in nematode relatives of Caenorhabditis elegans.
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Rivard, Laura, Srinivasan, Jagan, Stone, Allison, Ochoa, Stacy, Sternberg, Paul W, and Loer, Curtis M
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ANIMAL behavior ,NERVOUS system ,NEMATODES ,NEUROTRANSMITTERS ,CAENORHABDITIS elegans ,BACTERIA - Abstract
Background: Survival of an animal depends on its ability to match its responses to environmental conditions. To generate an optimal behavioral output, the nervous system must process sensory information and generate a directed motor output in response to stimuli. The nervous system should also store information about experiences to use in the future. The diverse group of free-living nematodes provides an excellent system to study macro- and microevolution of molecular, morphological and behavioral character states associated with such nervous system function. We asked whether an adaptive behavior would vary among bacterivorous nematodes and whether differences in the neurotransmitter systems known to regulate the behavior in one species would reflect differences seen in the adaptive behavior among those species. Caenorhabditis elegans worms slow in the presence of food; this 'basal' slowing is triggered by dopaminergic mechanosensory neurons that detect bacteria. Starved worms slow more dramatically; this 'enhanced' slowing is regulated by serotonin. Results: We examined seven nematode species with known phylogenetic relationship to C. elegans for locomotory behaviors modulated by food (E. coli), and by the worm's recent history of feeding (being well-fed or starved). We found that locomotory behavior in some species was modulated by food and recent feeding experience in a manner similar to C. elegans, but not all the species tested exhibited these food-modulated behaviors. We also found that some worms had different responses to bacteria other than E. coli. Using histochemical and immunological staining, we found that dopaminergic neurons were very similar among all species. For instance, we saw likely homologs of four bilateral pairs of dopaminergic cephalic and deirid neurons known from C. elegans in all seven species examined. In contrast, there was greater variation in the patterns of serotonergic neurons. The presence of presumptive homologs of dopaminergic and serotonergic neurons in a given species did not correlate with the observed differences in locomotory behaviors. Conclusions: This study demonstrates that behaviors can differ significantly between species that appear morphologically very similar, and therefore it is important to consider factors, such as ecology of a species in the wild, when formulating hypotheses about the adaptive significance of a behavior. Our results suggest that evolutionary changes in locomotory behaviors are less likely to be caused by changes in neurotransmitter expression of neurons. Such changes could be caused either by subtle changes in neural circuitry or in the function of the signal transduction pathways mediating these behaviors. [ABSTRACT FROM AUTHOR]
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- 2010
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20. Differences in transcription between free-living and CO2-activated third-stage larvae of Haemonchus contortus.
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Cantacessi, Cinzia, Campbell, Bronwyn E., Young, Neil D., Jex, Aaron R., Hall, Ross S., Presidente, Paul J. A., Zawadzki, Jodi L., Weiwei Zhong, Aleman-Meza, Boanerges, Loukas, Alex, Sternberg, Paul W., and Gasser, Robin B.
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HAEMONCHUS contortus ,NEMATODES ,RUMINANTS ,GENETIC transcription ,LARVAE - Abstract
Background: The disease caused by Haemonchus contortus, a blood-feeding nematode of small ruminants, is of major economic importance worldwide. The infective third-stage larva (L3) of this gastric nematode is enclosed in a cuticle (sheath) and, once ingested with herbage by the host, undergoes an exsheathment process that marks the transition from the free-living (L3) to the parasitic (xL3) stage. This study explored changes in gene transcription associated with this transition and predicted, based on comparative analysis, functional roles for key transcripts in the metabolic pathways linked to larval development. Results: Totals of 101,305 (L3) and 105,553 (xL3) expressed sequence tags (ESTs) were determined using 454 sequencing technology, and then assembled and annotated; the most abundant transcripts encoded transthyretinlike, calcium-binding EF-hand, NAD(P)-binding and nucleotide-binding proteins as well as homologues of Ancylostoma-secreted proteins (ASPs). Using an in silico-subtractive analysis, 560 and 685 sequences were shown to be uniquely represented in the L3 and xL3 stages, respectively; the transcripts encoded ribosomal proteins, collagens and elongation factors (in L3), and mainly peptidases and other enzymes of amino acid catabolism (in xL3). Caenorhabditis elegans orthologues of transcripts that were uniquely transcribed in each L3 and xL3 were predicted to interact with a total of 535 other genes, all of which were involved in embryonic development. Conclusion: The present study indicated that some key transcriptional alterations taking place during the transition from the L3 to the xL3 stage of H. contortus involve genes predicted to be linked to the development of neuronal tissue (L3 and xL3), formation of the cuticle (L3) and digestion of host haemoglobin (xL3). Future efforts using nextgeneration sequencing and bioinformatic technologies should provide the efficiency and depth of coverage required for the determination of the complete transcriptomes of different developmental stages and/or tissues of H. contortus as well as the genome of this important parasitic nematode. Such advances should lead to a significantly improved understanding of the molecular biology of H. contortus and, from an applied perspective, to novel methods of intervention. [ABSTRACT FROM AUTHOR]
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- 2010
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21. A shortcut to identifying small molecule signals that regulate behavior and development in Caenorhabditis elegans.
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Pungaliya, Chirag, Srinivasan, Jagan, Fox, Bennett W., Malik, Rabia U., Ludewig, Andreas H., Sternberg, Paul W., and Schroeder, Frank C.
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CAENORHABDITIS elegans genetics ,GLYCOSIDES ,PHEROMONES ,NEMATODES ,METABOLITES ,NUCLEAR magnetic resonance spectroscopy - Abstract
Small molecule metabolites play important roles in Caenorhabditis e!egans biology, but effective approaches for identifying their chemical structures are lacking. Recent studies revealed that a family of glycosides, the ascarosides, differentially regulate C. elegans development and behavior. Low concentrations of ascarosides attract males and thus appear to be part of the C. elegans sex pheromone, whereas higher concentrations induce developmental arrest at the dauer stage, an alternative, nonaging larval stage. The ascarosides act synergistically, which presented challenges for their identification via traditional activity-guided fractionation. As a result the chemical characterization of the dauer and male attracting pheromones remained incomplete. Here, we describe the identification of several additional pheromone components by using a recently developed NMR-spectroscopic approach, differential analysis by 2D NMR spectroscopy (DANS), which simplifies linking small molecule metabolites with their biological function. DANS-based comparison of wild-type C. el- egans and a signaling-deficient mutant, daf-22, enabled identification of 3 known and 4 previously undescribed ascarosides, including a compound that features a p-aminobenzoic acid subunit. Biological testing of synthetic samples of these compounds revealed additional evidence for synergy and provided insights into structure-activity relationships. Using a combination of the three most active ascarosides allowed full reconstitution of the male-attracting activity of wild-type pheromone extract. Our results highlight the efficacy of DANS as a method for identifying small-molecule metabolites and placing them within a specific genetic context. This study further supports the hypothesis that ascaro-sides represent a structurally diverse set of nematode signaling molecules regulating major life history traits. [ABSTRACT FROM AUTHOR]
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- 2009
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22. Transcriptomic analysis of the entomopathogenic nematode Heterorhabditis bacteriophora TTO1.
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Xiaodong Bai, Adams, Byron J., Ciche, Todd A., Clifton, Sandra, Gaugler, Randy, Hogenhout, Saskia A., Spieth, John, Sternberg, Paul W., Wilson, Richard K., and Grewal, Parwinder S.
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HETERORHABDITIS ,NEMATODES ,BACTERIAL genomes ,GENE expression ,INSECT nematodes - Abstract
Background: The entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterium, Photorhabdus luminescens, are important biological control agents of insect pests. This nematode-bacterium-insect association represents an emerging tripartite model for research on mutualistic and parasitic symbioses. Elucidation of mechanisms underlying these biological processes may serve as a foundation for improving the biological control potential of the nematode-bacterium complex. This large-scale expressed sequence tag (EST) analysis effort enables gene discovery and development of microsatellite markers. These ESTs will also aid in the annotation of the upcoming complete genome sequence of H. bacteriophora. Results: A total of 31,485 high quality ESTs were generated from cDNA libraries of the adult H. bacteriophora TTO1 strain. Cluster analysis revealed the presence of 3,051 contigs and 7,835 singletons, representing 10,886 distinct EST sequences. About 72% of the distinct EST sequences had significant matches (E value < 1e-5) to proteins in GenBank's non-redundant (nr) and Wormpep190 databases. We have identified 12 ESTs corresponding to 8 genes potentially involved in RNA interference, 22 ESTs corresponding to 14 genes potentially involved in dauer-related processes, and 51 ESTs corresponding to 27 genes potentially involved in defense and stress responses. Comparison to ESTs and proteins of free-living nematodes led to the identification of 554 parasitic nematode-specific ESTs in H. bacteriophora, among which are those encoding F-box-like/WDrepeat protein theromacin, Bax inhibitor-1-like protein, and PAZ domain containing protein. Gene Ontology terms were assigned to 6,685 of the 10,886 ESTs. A total of 168 microsatellite loci were identified with primers designable for 141 loci. Conclusion: A total of 10,886 distinct EST sequences were identified from adult H. bacteriophora cDNA libraries. BLAST searches revealed ESTs potentially involved in parasitism, RNA interference, defense responses, stress responses, and dauerrelated processes. The putative microsatellite markers identified in H. bacteriophora ESTs will enable genetic mapping and population genetic studies. These genomic resources provide the material base necessary for genome annotation, microarray development, and in-depth gene functional analysis. [ABSTRACT FROM AUTHOR]
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- 2009
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23. The Caenorhabditis elegans vulva: A post-embryonic gene regulatory network controlling organogenesis.
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Ririe, Ted O., Fernandes, Jolene S., and Sternberg, Paul W.
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GENETIC regulation ,GENE expression ,PROTEINS ,CELLS ,CAENORHABDITIS elegans ,MORPHOGENESIS ,TRANSCRIPTION factors ,NEMATODES ,ANIMAL morphology - Abstract
The Caenorhabditis elegans vulva is an elegant model for dissecting a gene regulatory network (GRN) that directs postembryonic organogenesis. The mature vulva comprises seven cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), each with its own unique pattern of spatial and temporal gene expression. The mechanisms that specify these cell types in a precise spatial pattern are not well understood. Using reverse genetic screens, we identified novel components of the vulval GRN, including nhr-113 in vulA. Several transcription factors (lin-11, lin-29, cog-1, egl-38, and nhr-67) interact with each other and act in concert to regulate target gene expression in the diverse vulval cell types. For example, egl-38 (Pax2/5/8) stabilizes the vulF fate by positively regulating vulF characteristics and by inhibiting characteristics associated with the neighboring vulE cells. nhr-67 and egl-38 regulate cog-1, helping restrict its expression to vulE. Computational approaches have been successfully used to identify functional cis-regulatory motifs in the zmp-1 (zinc metalloproteinase) promoter. These results provide an overview of the regulatory network architecture for each vulval cell type. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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24. EXPRESSED SEQUENCE TAG ANALYSIS OF GENE REPRESENTATION IN INSECT PARASITIC NEMATODE HETERORHABDITIS BACTERIOPHORA.
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Xiaodong Bai, Grewal, Parwinder S., Hogenhout, Saskia A., Adams, Byron J., Ciche, Todd A., RandyvGaugler, and Sternberg, Paul W.
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INSECT parasites ,NEMATODES ,HETERORHABDITIS ,PARASITISM ,HOST-parasite relationships - Abstract
We compared Heterorhabditis bacteriophora GPS11 expressed sequence tags (ESTs) to the ESTs of animal-parasitic, human-parasitic, plant-parasitic, and free-living nematodes. We identified 127 previously nondescribed ESTs of which 119 had homologs in ESTs and 8 had homologs in proteins of free-living nematodes. These ESTs were assigned putative functions in transcription, signal transduction, cell cycle control, metabolism, information processing, and cellular processes, thereby providing better insight into H. bacteriophora metabolism, sex determination, and signal transduction. We also identified 36 H. bacteriophora ESTs that had significant similarities to ESTs of parasitic nematodes, but not to ESTs or proteins of free-living nematodes species. Among these are the ESTs encoding a centrin, an ankyrin-repeat containing protein, and a nuclear hormone receptor. Our analysis also revealed that parasitic nematode-specific ESTs in this H. bacteriophora data set had more homologs in animalparasitic nematodes than those parasitizing humans or plants. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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25. The versatile worm: genetic and genomic resources for Caenorhabditis elegans research.
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Antoshechkin, Igor and Sternberg, Paul W.
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WORMS , *CAENORHABDITIS elegans , *RHABDITIDAE , *NEMATODES , *ORGANISMS - Abstract
Since its establishment as a model organism, Caenorhabditis elegans has been an invaluable tool for biological research. An immense spectrum of questions can be addressed using this small nematode, making it one of the most versatile and exciting model organisms. Although the many tools and resources developed by the C. elegans community greatly facilitate new discoveries, they can also overwhelm newcomers to the field. This Review aims to familiarize new worm researchers with the main resources, and help them to select the tools that are best suited for their needs. We also hope that it will be helpful in identifying new research opportunities and will promote the development of additional resources. [ABSTRACT FROM AUTHOR]
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- 2007
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26. Pathway to RAS.
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Sternberg, Paul W.
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CELL membranes , *RAS oncogenes , *GUANOSINE triphosphatase genes , *CAENORHABDITIS elegans , *NEMATODES , *WORMS - Abstract
The article examines the initial generic pathway from a cell surface receptor to the ras proto-oncogene let-60 in Caenorhabditis elegans. It was found that the vulvaless mutants that mimic the ablation of the anchor cell and the multivulva mutants in the precursor cells generate vulval tissue even in the absence of the anchor cell. These results imply the presence of a signaling pathway from the anchor cell to the precursor cells.
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- 2006
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27. An automated system for measuring parameters of nematode sinusoidal movement.
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Cronin, Christopher J, Mendel, Jane E, Mukhtar, Saleem, Young-Mee Kim, Stirbl, Robert C, Bruck, Jehoshua, and Sternberg, Paul W
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NEMATODES ,PHENOTYPES ,GENES ,PHYSIOLOGY ,SPEED - Abstract
Background: Nematode sinusoidal movement has been used as a phenotype in many studies of C. elegans development, behavior and physiology. A thorough understanding of the ways in which genes control these aspects of biology depends, in part, on the accuracy of phenotypic analysis. While worms that move poorly are relatively easy to describe, description of hyperactive movement and movement modulation presents more of a challenge. An enhanced capability to analyze all the complexities of nematode movement will thus help our understanding of how genes control behavior. Results: We have developed a user-friendly system to analyze nematode movement in an automated and quantitative manner. In this system nematodes are automatically recognized and a computer-controlled microscope stage ensures that the nematode is kept within the camera field of view while video images from the camera are stored on videotape. In a second step, the images from the videotapes are processed to recognize the worm and to extract its changing position and posture over time. From this information, a variety of movement parameters are calculated. These parameters include the velocity of the worm's centroid, the velocity of the worm along its track, the extent and frequency of body bending, the amplitude and wavelength of the sinusoidal movement, and the propagation of the contraction wave along the body. The length of the worm is also determined and used to normalize the amplitude and wavelength measurements. To demonstrate the utility of this system, we report here a comparison of movement parameters for a small set of mutants affecting the Go/Gq mediated signaling network that controls acetylcholine release at the neuromuscular junction. The system allows comparison of distinct genotypes that affect movement similarly (activation of Gq-alpha versus loss of Go-alpha function), as well as of different mutant alleles at a single locus (null and dominant negative alleles of the goa-1 gene, which encodes Goalpha). We also demonstrate the use of this system for analyzing the effects of toxic agents. Concentration-response curves for the toxicants arsenite and aldicarb, both of which affect motility, were determined for wild-type and several mutant strains, identifying P-glycoprotein mutants as not significantly more sensitive to either compound, while cat-4 mutants are more sensitive to arsenite but not aldicarb. Conclusions: Automated analysis of nematode movement facilitates a broad spectrum of experiments. Detailed genetic analysis of multiple alleles and of distinct genes in a regulatory network is now possible. These studies will facilitate quantitative modeling of C. elegans movement, as well as a comparison of gene function. Concentration-response curves will allow rigorous analysis of toxic agents as well as of pharmacological agents. This type of system thus represents a powerful analytical tool that can be readily coupled with the molecular genetics of nematodes. [ABSTRACT FROM AUTHOR]
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- 2005
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28. An imaging system for standardized quantitative analysis of C. elegans behavior.
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Zhaoyang Feng, Cronin, Christopher J., Wittig Jr., John H., Sternberg, Paul W., and Schafer, William R.
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IMAGING systems ,CAENORHABDITIS elegans ,NEMATODES ,PHENOTYPES ,CAENORHABDITIS - Abstract
Background: The nematode Caenorhabditis elegans is widely used for the genetic analysis of neuronal cell biology, development, and behavior. Because traditional methods for evaluating behavioral phenotypes are qualitative and imprecise, there is a need for tools that allow quantitation and standardization of C. elegans behavioral assays. Results: Here we describe a tracking and imaging system for the automated analysis of C. elegans morphology and behavior. Using this system, it is possible to record the behavior of individual nematodes over long time periods and quantify 144 specific phenotypic parameters. Conclusions: These tools for phenotypic analysis will provide reliable, comprehensive scoring of a wide range of behavioral abnormalities, and will make it possible to standardize assays such that behavioral data from different labs can readily be compared. In addition, this system will facilitate high-throughput collection of phenotypic data that can ultimately be used to generate a comprehensive database of C. elegans phenotypic information. Availability: The hardware configuration and software for the system are available from wschafer@ucsd.edu. [ABSTRACT FROM AUTHOR]
- Published
- 2004
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29. Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae).
- Author
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Ley, Paul De, Felix, Marie-Anne, Frisse, Linda M., Nadler, Steven A., Sternberg, Paul W., and Thomas, W. Kelley
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NEMATODES ,RHABDITIDA ,WORMS ,MORPHOLOGY - Abstract
Detailed descriptions are given of the amphimictic nematode strains PS1158, PS2052 and PS2160, which are unusual in that they only differ in predominant body handedness. Although these strains are morphologically identical in all other respects, published reproductive data and new DNA sequence data of the D2/D3 region of the large subunit rRNA gene show that they do represent two separate species. On the basis of comparison with type material, the left-handed strains PS1158 and PS2160 are identified as Acrobeloides bodenheimeri, and the right-handed strain PS2052 as A. camberenensis, which is re-instated as a valid species. A. bodenheimeri and its relatives exhibit various types of diagnostic and taxonomic problems at species level, and it is shown that D2/D3 sequence data provide an important new diagnostic tool for addressing these problems. Phylogenetic analysis shows that two right-handed parthenogenetic strains identified as A. maximus represent a third species which is more closely related to A. camberenensis than to A. bodenheimeri. Caracterisation morphologique et moleculaire de deux especes intersteriles de chiralite contraire (Nematoda: Cephalobidae) - Une description detaillee est donnee des souches amphimictiques de nematodes PS1158, PS2052 et PS2160, souches inhabituelles car differant par la chiralite du corps. Bien que ces souches soient morphologiquement identiques sous tout autre rapport, les resultats publies de tests de croisement et de nouvelles donnees concernant la sequence d'ADN de la region D2/D3 du gene de la grosse sous-unite d'ARN ribosomal montrent qu'elles representent en fait deux especes distinctes. Se fondant sur une comparaison avec le materiel type, les souches sinistres PS1158 et PS2160 sont identifiees comme Acrobeloides bodenheimeri et la souche dextre PS2052 comme A. camberenensis, ainsi retabli comme espece valide. A. bodenheimeri et les especes proches posent differents problemes diagnostiques et taxinomiques au niveau specifique, et nous montrons que les donnees de sequence D2/D3 fournissent un nouvel outil diagnostique important pour aborder ces problemes. L'analyse phylogenetique montre que deux souches parthenogenetiques dextres identifiees comme A. maximus representent en fait une troisieme espece, plus proche de A. camberenensis que de A. bodenheimeri. [ABSTRACT FROM AUTHOR]
- Published
- 1999
30. THE GENETIC CONTROL OF CELL LINEAGE DURING NEMATODE DEVELOPMENT.
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Sternberg, Paul W. and Horvitz, H. Robert
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GENETIC regulation , *GENE expression , *NEMATODES , *WORMS , *GENETICS , *ANIMAL genetics - Abstract
Explains the genetic control of cell lineage during the developmental stages of nematodes. Patterns of cell divisions and cell fates; Invariance and cell autonomy; Discussion of cell lineage mutants.
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- 1984
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31. Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematode Steinernema hermaphroditum.
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Schwartz, Hillel T, Tan, Chieh-Hsiang, Peraza, Jackeline, Raymundo, Krystal Louise T, and Sternberg, Paul W
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NEMATODES , *NEMATODE infections , *SEQUENCE analysis , *CAENORHABDITIS elegans , *PHENOMENOLOGICAL biology , *ANIMAL experimentation , *RESEARCH funding , *OXIDOREDUCTASES , *GENETIC techniques , *CRISPRS , *BODY size - Abstract
The entomopathogenic nematode Steinernema hermaphroditum was recently rediscovered and is being developed as a genetically tractable experimental system for the study of previously unexplored biology, including parasitism of its insect hosts and mutualism with its bacterial endosymbiont Xenorhabdus griffiniae. Through whole-genome re-sequencing and genetic mapping we have for the first time molecularly identified the gene responsible for a mutationally defined phenotypic locus in an entomopathogenic nematode. In the process we observed an unexpected mutational spectrum following ethyl methansulfonate mutagenesis in this species. We find that the ortholog of the essential Caenorhabditis elegans peroxidase gene skpo-2 controls body size and shape in S. hermaphroditum. We confirmed this identification by generating additional loss-of-function mutations in the gene using CRISPR-Cas9. We propose that the identification of skpo-2 will accelerate gene targeting in other Steinernema entomopathogenic nematodes used commercially in pest control, as skpo-2 is X-linked and males hemizygous for loss of its function can mate, making skpo-2 an easily recognized and maintained marker for use in co-CRISPR. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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32. Pristionchus pacificus: an appropriate fondness for beetles.
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Srinivasan, Jagan and Sternberg, Paul W.
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GENETICS , *NUCLEOTIDE sequence , *PARASITISM , *BEETLES , *NEMATODES , *INSECTS - Abstract
The nematode Pristionchus pacificus associates with one particular beetle and eats its rotting corpse. The report of the genome sequence of P. pacificus, the fifth nematode to be sequenced and a useful secondary nematode genetic model system, highlights genes that may have influenced the route to parasitism. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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33. A Pattern of Precision.
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Sternberg, Paul W.
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CELL determination , *CAENORHABDITIS elegans , *NEMATODES , *EPIDERMAL growth factor , *FEMALE reproductive organs , *CELLS - Abstract
Models of cell fate patterning during development emphasize the contrast between spatial gradients of developmental signals that act at long range and cell-to-cell signaling events that act locally. Development of the vulva in the nematode Caenorhabditis elegans provides an elegant model system for examining the patterning of cell fate in an animal. There is strong evidence that two different intercellular signals contribute to the relatively simple induction of cell fate among vulval precursor cells and a long-range spatial gradient of epidermal growth factor.
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- 2004
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34. Formation and function of dauer ascarosides in the nematodes Caenorhabditis briggsae and Caenorhabditis elegans.
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Cohen, Sarah M., Wrobel, Chester J. J., Prakash, Sharan J., Schroeder, Frank C., and Sternberg, Paul W.
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CAENORHABDITIS elegans , *CAENORHABDITIS , *NEMATODES - Abstract
The biosynthetic pathways and functions of ascaroside signaling molecules in the nematode Caenorhabditis elegans have been studied to better understand complex, integrative developmental decision-making. Although it is known that ascarosides play multiple roles in the development and behavior of nematode species other than C. elegans, these parallel pheromone systems have not been well-studied. Here, we show that ascarosides in the nematode Caenorhabditis briggsae are biosynthesized in the same manner as C. elegans and act to induce the alternative developmental pathway that generates the stress-resistant dauer lifestage. We show that ascr#2 is the primary component of crude dauer pheromone in C. briggsae; in contrast, C. elegans dauer pheromone relies on a combination of ascr#2, ascr#3, and several other components. We further demonstrate that Cbr-daf-22, like its C. elegans ortholog Cel-daf-22, is necessary to produce short-chain ascarosides. Moreover, Cbr-daf-22 and Cel-daf-22 mutants produce an ascaroside-independent metabolite that acts antagonistically to crude dauer pheromone and inhibits dauer formation. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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35. The entomopathogenic nematode Steinernema hermaphroditum is a self-fertilizing hermaphrodite and a genetically tractable system for the study of parasitic and mutualistic symbiosis.
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Mengyi Cao, Schwartz, Hillel T., Chieh-Hsiang Tan, and Sternberg, Paul W.
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INSECT microbiology , *GUT microbiome , *IN vitro studies , *BIOLOGICAL models , *CHROMOSOMES , *NEMATODES , *GENETICS , *PARASITOLOGY , *GENETIC mutation , *IN vivo studies , *CONCEPTION , *PHENOMENOLOGICAL biology , *ANIMAL experimentation , *SEX chromosomes , *GENETIC testing , *ENTEROBACTERIACEAE , *GENETIC markers , *DESCRIPTIVE statistics , *PARASITES , *CRYOPRESERVATION of organs, tissues, etc. , *PHENOTYPES - Abstract
Entomopathogenic nematodes (EPNs), including Heterorhabditis and Steinernema, are parasitic to insects and contain mutualistically symbiotic bacteria in their intestines (Photorhabdus and Xenorhabdus, respectively) and therefore offer opportunities to study both mutualistic and parasitic symbiosis. The establishment of genetic tools in EPNs has been impeded by limited genetic tractability, inconsistent growth in vitro, variable cryopreservation, and low mating efficiency. We obtained the recently described Steinernema hermaphroditum strain CS34 and optimized its in vitro growth, with a rapid generation time on a lawn of its native symbiotic bacteria Xenorhabdus griffiniae. We developed a simple and efficient cryopreservation method. Previously, S. hermaphroditum isolated from insect hosts was described as producing hermaphrodites in the first generation. We discovered that CS34, when grown in vitro, produced consecutive generations of autonomously reproducing hermaphrodites accompanied by rare males. We performed mutagenesis screens in S. hermaphroditum that produced mutant lines with visible and heritable phenotypes. Genetic analysis of the mutants demonstrated that this species reproduces by self-fertilization rather than parthenogenesis and that its sex is determined chromosomally. Genetic mapping has thus far identified markers on the X chromosome and three of four autosomes. We report that S. hermaphroditum CS34 is the first consistently hermaphroditic EPN and is suitable for genetic model development to study naturally occurring mutualistic symbiosis and insect parasitism. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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36. Elucidating the molecular and developmental biology of parasitic nematodes: Moving to a multiomics paradigm.
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Ma, Guangxu, Wang, Tao, Korhonen, Pasi K., Hofmann, Andreas, Sternberg, Paul W., Young, Neil D., and Gasser, Robin B.
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- *
CAENORHABDITIS elegans , *DEVELOPMENTAL biology , *MOLECULAR biology , *NEMATODES , *HAEMONCHUS contortus , *FUNCTIONAL genomics , *HELMINTHS - Abstract
In the past two decades, significant progress has been made in the sequencing, assembly, annotation and analyses of genomes and transcriptomes of parasitic worms of socioeconomic importance. This progress has somewhat improved our knowledge and understanding of these pathogens at the molecular level. However, compared with the free-living nematode Caenorhabditis elegans, the areas of functional genomics, transcriptomics, proteomics and metabolomics of parasitic nematodes are still in their infancy, and there are major gaps in our knowledge and understanding of the molecular biology of parasitic nematodes. The information on signalling molecules, molecular pathways and microRNAs (miRNAs) that are known to be involved in developmental processes in C. elegans and the availability of some molecular resources (draft genomes, transcriptomes and some proteomes) for selected parasitic nematodes provide a basis to start exploring the developmental biology of parasitic nematodes. Indeed, some studies have identified molecules and pathways that might associate with developmental processes in related, parasitic nematodes, such as Haemonchus contortus (barber's pole worm). However, detailed information is often scant and 'omics resources are limited, preventing a proper integration of 'omic data sets and comprehensive analyses. Moreover, little is known about the functional roles of pheromones, hormones, signalling pathways and post-transcriptional/post-translational regulations in the development of key parasitic nematodes throughout their entire life cycles. Although C. elegans is an excellent model to assist molecular studies of parasitic nematodes, its use is limited when it comes to explorations of processes that are specific to parasitism within host animals. A deep understanding of parasitic nematodes, such as H. contortus, requires substantially enhanced resources and the use of integrative 'omics approaches for analyses. The improved genome and well-established in vitro larval culture system for H. contortus provide unprecedented opportunities for comprehensive studies of the transcriptomes (mRNA and miRNA), proteomes (somatic, excretory/secretory and phosphorylated proteins) and lipidomes (e.g., polar and neutral lipids) of this nematode. Such resources should enable in-depth explorations of its developmental biology at a level, not previously possible. The main aims of this review are (i) to provide a background on the development of nematodes, with a particular emphasis on the molecular aspects involved in the dauer formation and exit in C. elegans; (ii) to critically appraise the current state of knowledge of the developmental biology of parasitic nematodes and identify key knowledge gaps; (iii) to cover salient aspects of H. contortus, with a focus on the recent advances in genomics, transcriptomics, proteomics and lipidomics as well as in vitro culturing systems; (iv) to review recent advances in our knowledge and understanding of the molecular and developmental biology of H. contortus using an integrative multiomics approach, and discuss the implications of this approach for detailed explorations of signalling molecules, molecular processes and pathways likely associated with nematode development, adaptation and parasitism, and for the identification of novel intervention targets against these pathogens. Clearly, the multiomics approach established recently is readily applicable to exploring a wide range of interesting and socioeconomically significant parasitic worms (including also trematodes and cestodes) at the molecular level, and to elucidate host-parasite interactions and disease processes. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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37. Whipworm kinomes reflect a unique biology and adaptation to the host animal.
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Stroehlein, Andreas J., Young, Neil D., Korhonen, Pasi K., Chang, Bill C.H., Nejsum, Peter, Pozio, Edoardo, La Rosa, Giuseppe, Sternberg, Paul W., and Gasser, Robin B.
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WHIPWORMS , *NEMATODES , *HELMINTHS , *MOLECULAR biology , *TRICHURIDAE - Abstract
Roundworms belong to a diverse phylum (Nematoda) which is comprised of many parasitic species including whipworms (genus Trichuris ). These worms have adapted to a biological niche within the host and exhibit unique morphological characteristics compared with other nematodes. Although these adaptations are known, the underlying molecular mechanisms remain elusive. The availability of genomes and transcriptomes of some whipworms now enables detailed studies of their molecular biology. Here, we defined and curated the full complement of an important class of enzymes, the protein kinases (kinomes) of two species of Trichuris , using an advanced and integrated bioinformatic pipeline. We investigated the transcription of Trichuris suis kinase genes across developmental stages, sexes and tissues, and reveal that selectively transcribed genes can be linked to central roles in developmental and reproductive processes. We also classified and functionally annotated the curated kinomes by integrating evidence from structural modelling and pathway analyses, and compared them with other curated kinomes of phylogenetically diverse nematode species. Our findings suggest unique adaptations in signalling processes governing worm morphology and biology, and provide an important resource that should facilitate experimental investigations of kinases and the biology of signalling pathways in nematodes. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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38. The barber's pole worm CAP protein superfamily — A basis for fundamental discovery and biotechnology advances.
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Mohandas, Namitha, Young, Neil D., Jabbar, Abdul, Korhonen, Pasi K., Koehler, Anson V., Amani, Parisa, Hall, Ross S., Sternberg, Paul W., Jex, Aaron R., Hofmann, Andreas, and Gasser, Robin B.
- Subjects
- *
HAEMONCHUS contortus , *IMMUNE response , *BIOTECHNOLOGY , *HELMINTHS , *CELLULAR signal transduction , *TRANSFORMING growth factors-beta - Abstract
Parasitic worm proteins that belong to the cysteine-rich secretory proteins, antigen 5 and pathogenesis-related 1 (CAP) superfamily are proposed to play key roles in the infection process and the modulation of immune responses in host animals. However, there is limited information on these proteins for most socio-economically important worms. Here, we review the CAP protein superfamily of Haemonchus contortus (barber's pole worm), a highly significant parasitic roundworm (order Strongylida) of small ruminants. To do this, we mined genome and transcriptomic datasets, predicted and curated full-length amino acid sequences (n = 45), undertook systematic phylogenetic analyses of these data and investigated transcription throughout the life cycle of H. contortus . We inferred functions for selected Caenorhabditis elegans orthologs (including vap-1 , vap-2 , scl-5 and lon-1 ) based on genetic networking and by integrating data and published information, and were able to infer that a subset of orthologs and their interaction partners play pivotal roles in growth and development via the insulin-like and/or the TGF-beta signalling pathways. The identification of the important and conserved growth regulator LON-1 led us to appraise the three-dimensional structure of this CAP protein by comparative modelling. This model revealed the presence of different topological moieties on the canonical fold of the CAP domain, which coincide with an overall charge separation as indicated by the electrostatic surface potential map. These observations suggest the existence of separate sites for effector binding and receptor interactions, and thus support the proposal that these worm molecules act in similar ways as venoms act as ligands for chemokine receptors or G protein-coupled receptor effectors. In conclusion, this review should guide future molecular studies of these molecules, and could support the development of novel interventions against haemonchosis. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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39. Cracking the nodule worm code advances knowledge of parasite biology and biotechnology to tackle major diseases of livestock.
- Author
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Tyagi, Rahul, Joachim, Anja, Ruttkowski, Bärbel, Rosa, Bruce A., Martin, John C., Hallsworth-Pepin, Kymberlie, Zhang, Xu, Ozersky, Philip, Wilson, Richard K., Ranganathan, Shoba, Sternberg, Paul W., Gasser, Robin B., and Mitreva, Makedonka
- Subjects
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BIOTECHNOLOGY research , *COMMUNICABLE diseases , *MOLECULAR biology , *NEMATODES , *ANIMAL health , *LIVESTOCK , *ANIMAL behavior ,PARASITE physiology - Abstract
Many infectious diseases caused by eukaryotic pathogens have a devastating, long-term impact on animal health and welfare. Hundreds of millions of animals are affected by parasitic nematodes of the order Strongylida. Unlocking the molecular biology of representatives of this order, and understanding nematode–host interactions, drug resistance and disease using advanced technologies could lead to entirely new ways of controlling the diseases that they cause. Oesophagostomum dentatum (nodule worm; superfamily Strongyloidea) is an economically important strongylid nematode parasite of swine worldwide. The present article reports recent advances made in biology and animal biotechnology through the draft genome and developmental transcriptome of O. dentatum , in order to support biological research of this and related parasitic nematodes as well as the search for new and improved interventions. This first genome of any member of the Strongyloidea is 443 Mb in size and predicted to encode 25,291 protein-coding genes. Here, we review the dynamics of transcription throughout the life cycle of O. dentatum , describe double-stranded RNA interference (RNAi) machinery and infer molecules involved in development and reproduction, and in inducing or modulating immune responses or disease. The secretome predicted for O. dentatum is particularly rich in peptidases linked to interactions with host tissues and/or feeding activity, and a diverse array of molecules likely involved in immune responses. This research progress provides an important resource for future comparative genomic and molecular biological investigations as well as for biotechnological research toward new anthelmintics, vaccines and diagnostic tests. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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40. Bioinformatic exploration of RIO protein kinases of parasitic and free-living nematodes.
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Breugelmans, Bert, Jex, Aaron R., Korhonen, Pasi K., Mangiola, Stefano, Young, Neil D., Sternberg, Paul W., Boag, Peter R., Hofmann, Andreas, and Gasser, Robin B.
- Subjects
- *
OPEN reading frames (Genetics) , *PROTEIN kinases , *NEMATODES , *AMINO acid sequence , *BIOINFORMATICS , *THREE-dimensional imaging - Abstract
Despite right open reading frame kinases (RIOKs) being essential for life, their functions, substrates and cellular pathways remain enigmatic. In the present study, gene structures were characterised for 26 RIOKs from draft genomes of parasitic and free-living nematodes. RNA-seq transcription profiles of riok genes were investigated for selected parasitic nematodes and showed that these kinases are transcribed in developmental stages that infect their mammalian host. Three-dimensional structural models of Caenorhabditis elegans RIOKs were predicted, and elucidated functional domains and conserved regions in nematode homologs. These findings provide prospects for functional studies of riok genes in C. elegans , and an opportunity for the design and validation of nematode-specific inhibitors of these enzymes in socioeconomic parasitic worms. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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41. Insights into the immuno-molecular biology of Angiostrongylus vasorum through transcriptomics—Prospects for new interventions.
- Author
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Ansell, Brendan R.E., Schnyder, Manuela, Deplazes, Peter, Korhonen, Pasi K., Young, Neil D., Hall, Ross S., Mangiola, Stefano, Boag, Peter R., Hofmann, Andreas, Sternberg, Paul W., Jex, Aaron R., and Gasser, Robin B.
- Subjects
- *
MOLECULAR biology , *NEMATODES , *CANINE heartworm disease , *IMMUNE response , *CAENORHABDITIS elegans , *NUCLEOTIDE sequence , *GENETIC code - Abstract
Abstract: Angiostrongylus vasorum is a metastrongyloid nematode of dogs and other canids of major clinical importance in many countries. In order to gain first insights into the molecular biology of this worm, we conducted the first large-scale exploration of its transcriptome, and predicted essential molecules linked to metabolic and biological processes as well as host immune responses. We also predicted and prioritized drug targets and drug candidates. Following Illumina sequencing (RNA-seq), 52.3million sequence reads representing adult A. vasorum were assembled and annotated. The assembly yielded 20,033 contigs, which encoded proteins with 11,505 homologues in Caenorhabditis elegans, and additional 2252 homologues in various other parasitic helminths for which curated data sets were publicly available. Functional annotation was achieved for 11,752 (58.6%) proteins predicted for A. vasorum, including peptidases (4.5%) and peptidase inhibitors (1.6%), protein kinases (1.7%), G protein-coupled receptors (GPCRs) (1.5%) and phosphatases (1.2%). Contigs encoding excretory/secretory and immuno-modulatory proteins represented some of the most highly transcribed molecules, and encoded enzymes that digest haemoglobin were conserved between A. vasorum and other blood-feeding nematodes. Using an essentiality-based approach, drug targets, including neurotransmitter receptors, an important chemosensory ion channel and cysteine proteinase-3 were predicted in A. vasorum, as were associated small molecular inhibitors/activators. Future transcriptomic analyses of all developmental stages of A. vasorum should facilitate deep explorations of the molecular biology of this important parasitic nematode and support the sequencing of its genome. These advances will provide a foundation for exploring immuno-molecular aspects of angiostrongylosis and have the potential to underpin the discovery of new methods of intervention. [Copyright &y& Elsevier]
- Published
- 2013
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42. Nematode-Trapping Fungi Eavesdrop on Nematode Pheromones
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Hsueh, Yen-Ping, Mahanti, Parag, Schroeder, Frank C., and Sternberg, Paul W.
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NEMATODES , *PHEROMONES , *MOLECULAR recognition , *CELLULAR recognition , *PATHOGENIC fungi , *PREDATION , *BIOLOGICAL evolution - Abstract
Summary: The recognition of molecular patterns associated with specific pathogens or food sources is fundamental to ecology and plays a major role in the evolution of predator-prey relationships [1]. Recent studies showed that nematodes produce an evolutionarily highly conserved family of small molecules, the ascarosides, which serve essential functions in regulating nematode development and behavior [2–4]. Here, we show that nematophagous fungi, natural predators of soil-dwelling nematodes [5], can detect and respond to ascarosides. Nematophagous fungi use specialized trapping devices to catch and consume nematodes, and previous studies demonstrated that most fungal species do not produce traps constitutively but rather initiate trap formation in response to their prey [6]. We found that ascarosides, which are constitutively secreted by many species of soil-dwelling nematodes, represent a conserved molecular pattern used by nematophagous fungi to detect prey and trigger trap formation. Ascaroside-induced morphogenesis is conserved in several closely related species of nematophagous fungi and occurs only under nutrient-deprived conditions. Our results demonstrate that microbial predators eavesdrop on chemical communication among their metazoan prey to regulate morphogenesis, providing a striking example of predator-prey coevolution. We anticipate that these findings will have broader implications for understanding other interkingdom interactions involving nematodes, which are found in almost any ecological niche on Earth. [Copyright &y& Elsevier]
- Published
- 2013
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43. Ascaroside Signaling Is Widely Conserved among Nematodes
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Choe, Andrea, von Reuss, Stephan H., Kogan, Dima, Gasser, Robin B., Platzer, Edward G., Schroeder, Frank C., and Sternberg, Paul W.
- Subjects
- *
NEMATODES , *PHEROMONES , *PATHOGENIC microorganisms , *CAENORHABDITIS elegans , *MASS spectrometry , *LACTONES - Abstract
Summary: Background: Nematodes are among the most successful animals on earth and include important human pathogens, yet little is known about nematode pheromone systems. A group of small molecules called ascarosides has been found to mediate mate finding, aggregation, and developmental diapause in Caenorhabditis elegans, but it is unknown whether ascaroside signaling exists outside of the genus Caenorhabditis. Results: To determine whether ascarosides are used as signaling molecules by other nematode species, we performed a mass spectrometry-based screen for ascarosides in secretions from a variety of both free-living and parasitic (plant, insect, and animal) nematodes. We found that most of the species analyzed, including nematodes from several different clades, produce species-specific ascaroside mixtures. In some cases, ascaroside biosynthesis patterns appear to correlate with phylogeny, whereas in other cases, biosynthesis seems to correlate with lifestyle and ecological niche. We further show that ascarosides mediate distinct nematode behaviors, such as retention, avoidance, and long-range attraction, and that different nematode species respond to distinct, but overlapping, sets of ascarosides. Conclusions: Our findings indicate that nematodes utilize a conserved family of signaling molecules despite having evolved to occupy diverse ecologies. Their structural features and level of conservation are evocative of bacterial quorum sensing, where acyl homoserine lactones (AHLs) are both produced and sensed by many species of gram-negative bacteria. The identification of species-specific ascaroside profiles may enable pheromone-based approaches to interfere with reproduction and survival of parasitic nematodes, which are responsible for significant agricultural losses and many human diseases worldwide. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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44. Evolutionary conservation of cell migration genes: from nematode neurons to vertebrate neural crest.
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Yun Kee, Byung Joon Hwang, Sternberg, Paul W., and Bronner-Fraser, Marianne
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CELL migration , *NEMATODES , *NEURAL crest , *CAENORHABDITIS elegans , *ANIMAL genetics , *VERTEBRATES , *INVERTEBRATES - Abstract
Because migratory cells in all animals share common properties, we hypothesized that genetic networks involved in cell migration may be conserved between nematodes and vertebrates. To explore this, we performed comparative genomic analysis to identify vertebrate orthologs of genes required for hermaphrodite-specific neuron (HSN) migration in Caenoryhabditis elegans, and then examined their expression and function in the vertebrate neural crest. The results demonstrate high conservation of regulatory components involved in long-range migrations across diverse species. Although the neural crest is a vertebrate innovation, the results suggest that its migratory properties evolved by utilizing programs already present in the common vertebrate--invertebrate ancestor. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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45. Deep insights into Dictyocaulus viviparus transcriptomes provides unique prospects for new drug targets and disease intervention
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Cantacessi, Cinzia, Gasser, Robin B., Strube, Christina, Schnieder, Thomas, Jex, Aaron R., Hall, Ross S., Campbell, Bronwyn E., Young, Neil D., Ranganathan, Shoba, Sternberg, Paul W., and Mitreva, Makedonka
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DICTYOCAULUS , *VIVIPARUS , *DRUG target , *LUNGWORMS , *BRONCHITIS , *PROTEIN kinases , *HEALTH outcome assessment , *NEMATODES , *ANCYLOSTOMA , *BIOINFORMATICS - Abstract
Abstract: The lungworm, Dictyocaulus viviparus, causes parasitic bronchitis in cattle, and is responsible for substantial economic losses in temperate regions of the world. Here, we undertake the first large-scale exploration of available transcriptomic data for this lungworm, examine differences in transcription between different stages/both genders and identify and prioritize essential molecules linked to fundamental metabolic pathways, which could represent novel drug targets. Approximately 3 million expressed sequence tags (ESTs), generated by 454 sequencing from third-stage larvae (L3s) as well as adult females and males of D. viviparus, were assembled and annotated. The assembly of these sequences yielded ~61,000 contigs, of which relatively large proportions encoded collagens (4.3%), ubiquitins (2.1%) and serine/threonine protein kinases (1.9%). Subtractive analysis in silico identified 6928 nucleotide sequences as being uniquely transcribed in L3, and 5203 and 7889 transcripts as being exclusive to the adult female and male, respectively. Most peptides predicted from the conceptual translations were nucleoplasmins (L3), serine/threonine protein kinases (female) and major sperm proteins (male). Additional analyses allowed the prediction of three drug target candidates, whose Caenorhabditis elegans homologues were linked to a lethal RNA interference phenotype. This detailed exploration, combined with future transcriptomic sequencing of all developmental stages of D. viviparus, will facilitate future investigations of the molecular biology of this parasitic nematode as well as genomic sequencing. These advances will underpin the discovery of new drug and/or vaccine targets, focused on biotechnological outcomes. [Copyright &y& Elsevier]
- Published
- 2011
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46. Atypical (RIO) protein kinases from Haemonchus contortus — Promise as new targets for nematocidal drugs
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Campbell, Bronwyn E., Boag, Peter R., Hofmann, Andreas, Cantacessi, Cinzia, Wang, Conan K., Taylor, Paul, Hu, Min, Sindhu, Zia-ud-Din, Loukas, Alex, Sternberg, Paul W., and Gasser, Robin B.
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PROTEIN kinases , *HAEMONCHUS contortus , *ORGANISMS , *PARASITES , *NEMATODES , *CAENORHABDITIS elegans , *EUKARYOTIC cells , *RUMINANTS , *TARGETED drug delivery - Abstract
Abstract: Almost nothing is known about atypical kinases in multicellular organisms, including parasites. Supported by information and data available for the free-living nematode, Caenorhabditis elegans, and other eukaryotes, the present article describes three RIO kinase genes, riok-1, riok-2 and riok-3, from Haemonchus contortus, one of the most important parasitic nematodes of small ruminants. Analyses of these genes and their products predict that they each play critical roles in the developmental pathways of parasitic nematodes. The findings of this review indicate prospects for functional studies of these genes in C. elegans (as a surrogate) and opportunities for the design of a novel class of nematode-specific inhibitors of RIO kinases. The latter aspect is of paramount importance, given the serious problems linked to anthelmintic resistance in parasitic nematode populations of livestock. [Copyright &y& Elsevier]
- Published
- 2011
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47. A Sensory Code for Host Seeking in Parasitic Nematodes
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Hallem, Elissa A., Dillman, Adler R., Hong, Annie V., Zhang, Yuanjun, Yano, Jessica M., DeMarco, Stephanie F., and Sternberg, Paul W.
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NEMATODES , *HOSTS (Biology) , *SENSE organs , *PARASITES , *HETERORHABDITIS , *STEINERNEMATIDAE , *CARBON dioxide - Abstract
Summary: Parasitic nematode species often display highly specialized host-seeking behaviors that reflect their specific host preferences. Many such behaviors are triggered by host odors, but little is known about either the specific olfactory cues that trigger these behaviors or the underlying neural circuits. Heterorhabditis bacteriophora and Steinernema carpocapsae are phylogenetically distant insect-parasitic nematodes whose host-seeking and host-invasion behavior resembles that of some devastating human- and plant-parasitic nematodes. We compare the olfactory responses of Heterorhabditis and Steinernema infective juveniles (IJs) to those of Caenorhabditis elegans dauers, which are analogous life stages []. The broad host range of these parasites results from their ability to respond to the universally produced signal carbon dioxide (CO2), as well as a wide array of odors, including host-specific odors that we identified using thermal desorption-gas chromatography-mass spectroscopy. We find that CO2 is attractive for the parasitic IJs and C. elegans dauers despite being repulsive for C. elegans adults [], and we identify a sensory neuron that mediates CO2 response in both parasitic and free-living species, regardless of whether CO2 is attractive or repulsive. The parasites'' odor response profiles are more similar to each other than to that of C. elegans despite their greater phylogenetic distance, likely reflecting evolutionary convergence to insect parasitism. [Copyright &y& Elsevier]
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- 2011
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48. Elucidating ANTs in worms using genomic and bioinformatic tools — Biotechnological prospects?
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Hu, Min, Zhong, Weiwei, Campbell, Bronwyn E., Sternberg, Paul W., Pellegrino, Mark W., and Gasser, Robin B.
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BIOINFORMATICS , *BIOTECHNOLOGY , *ADENINE nucleotides , *LARVAE , *POLYMERASE chain reaction , *ANTISENSE DNA , *NEMATODES , *CAENORHABDITIS elegans - Abstract
Abstract: Adenine nucleotide translocators (ANTs) belong to the mitochondrial carrier family (MCF) of proteins. ATP production and consumption are tightly linked to ANTs, the kinetics of which have been proposed to play a key regulatory role in mitochondrial oxidative phosphorylation. ANTs are also recognized as a central component of the mitochondrial permeability transition pore associated with apoptosis. Although ANTs have been investigated in a range of vertebrates, including human, mouse and cattle, and invertebrates, such as Drosophila melanogaster (vinegar fly), Saccharomyces cerevisiae (yeast) and Caenorhabditis elegans (free-living nematode), there has been a void of information on these molecules for parasitic nematodes of socio-economic importance. Exploring ANTs in nematodes has the potential lead to a better understanding of their fundamental roles in key biological pathways and might provide an avenue for the identification of targets for the rational design of nematocidal drugs. In the present article, we describe the discovery of an ANT from Haemonchus contortus (one of the most economically important parasitic nematodes of sheep and goats), conduct a comparative analysis of key ANTs and their genes (particularly ant-1.1) in nematodes and other organisms, predict the functional roles utilizing a combined genomic-bioinformatic approach and propose ANTs and associated molecules as possible drug targets, with the potential for biotechnological outcomes. [Copyright &y& Elsevier]
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- 2010
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49. Wnt and EGF pathways act together to induce C. elegans male hook development
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Yu, Hui, Seah, Adeline, Herman, Michael A., Ferguson, Edwin L., Horvitz, H. Robert, and Sternberg, Paul W.
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DEVELOPMENTAL genetics , *VULVA , *EPIDERMAL growth factor , *CAENORHABDITIS elegans , *INTERSEXUALITY in animals , *COMPARATIVE studies , *DEVELOPMENTAL biology , *NEMATODES , *PHYSIOLOGY - Abstract
Abstract: Comparative studies of vulva development between Caenorhabditis elegans and other nematode species have provided some insight into the evolution of patterning networks. However, molecular genetic details are available only in C. elegans and Pristionchus pacificus. To extend our knowledge on the evolution of patterning networks, we studied the C. elegans male hook competence group (HCG), an equivalence group that has similar developmental origins to the vulval precursor cells (VPCs), which generate the vulva in the hermaphrodite. Similar to VPC fate specification, each HCG cell adopts one of three fates (1°, 2°, 3°), and 2° HCG fate specification is mediated by LIN-12/Notch. We show that 2° HCG specification depends on the presence of a cell with the 1° fate. We also provide evidence that Wnt signaling via the Frizzled-like Wnt receptor LIN-17 acts to specify the 1° and 2° HCG fate. A requirement for EGF signaling during 1° fate specification is seen only when LIN-17 activity is compromised. In addition, activation of the EGF pathway decreases dependence on LIN-17 and causes ectopic hook development. Our results suggest that WNT plays a more significant role than EGF signaling in specifying HCG fates, whereas in VPC specification EGF signaling is the major inductive signal. Nonetheless, the overall logic is similar in the VPCs and the HCG: EGF and/or WNT induce a 1° lineage, and LIN-12/NOTCH induces a 2° lineage. Wnt signaling is also required for execution of the 1° and 2° HCG lineages. lin-17 and bar-1/β-catenin are preferentially expressed in the presumptive 1° cell P11.p. The dynamic subcellular localization of BAR-1–GFP in P11.p is concordant with the timing of HCG fate determination. [Copyright &y& Elsevier]
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- 2009
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50. Nematodes, Bacteria, and Flies: A Tripartite Model for Nematode Parasitism
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Hallem, Elissa A., Rengarajan, Michelle, Ciche, Todd A., and Sternberg, Paul W.
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NEMATODES , *PARASITES , *DROSOPHILA , *PARASITOLOGY - Abstract
Summary: More than a quarter of the world''s population is infected with nematode parasites, and more than a hundred species of nematodes are parasites of humans . Despite extensive morbidity and mortality caused by nematode parasites, the biological mechanisms of host-parasite interactions are poorly understood, largely because of the lack of genetically tractable model systems. We have demonstrated that the insect parasitic nematode Heterorhabditis bacteriophora, its bacterial symbiont Photorhabdus luminescens, and the fruit fly Drosophila melanogaster constitute a tripartite model for nematode parasitism and parasitic infection. We find that infective juveniles (IJs) of Heterorhabditis, which contain Photorhabdus in their gut, can infect and kill Drosophila larvae. We show that infection activates an immune response in Drosophila that results in the temporally dynamic expression of a subset of antimicrobial peptide (AMP) genes, and that this immune response is induced specifically by Photorhabdus. We also investigated the cellular and molecular mechanisms underlying IJ recovery, the developmental process that occurs in parasitic nematodes upon host invasion and that is necessary for successful parasitism. We find that the chemosensory neurons and signaling pathways that control dauer recovery in Caenorhabditis elegans also control IJ recovery in Heterorhabditis, suggesting conservation of these developmental processes across free-living and parasitic nematodes. [Copyright &y& Elsevier]
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- 2007
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