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Abstract: Objective: This is the first attempt for an efficient plant regeneration protocol through in vitro direct organogenesis for a valuable medicinal plant, Dipteracanthus prostratus using nodal segment. Methods: Multiple shoots were induced from nodal explants cultured on Murashige and Skoog medium supplemented with kinetin (KIN), 6-benzylaminopurine (BAP) and 1-Naphthaleneacetic acid (NAA). Results: Maximum shoot responses (80%) were obtained with kinetin at 1.0 mg−1. The rate of shoot multiplication was maintained in subsequent subculture on similar fresh culture medium. The highest shoot length (3.96cm) was obtained with seventy three percentages of shoots at 0.2 mg−1 NAA along with 1.0 mg−1 kinetin. Maximum length of root (3.63cm) was formed at 0.5 mg−1 IBA with significant responses (80%). Rooted plantlets were then transferred to perforated plastic cups and grown in the green house at 80% survival rate. Conclusions: The highest survival rate was noticed and this plant developmental protocol could be used for large— scale regeneration of D. prostratus. [Copyright &y& Elsevier]