1. Structural and Functional Analysis of the Spermatogenic Epithelium in Rats Exposed to Titanium Dioxide Nanoparticles.
- Author
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Sharafutdinova LA, Fedorova AM, Bashkatov SA, Sinel'nikov KN, and Valiullin VV
- Subjects
- Administration, Oral, Animals, Biomarkers metabolism, Cell Differentiation drug effects, Cell Proliferation drug effects, Ki-67 Antigen metabolism, Leydig Cells metabolism, Leydig Cells ultrastructure, Male, Nanoparticles chemistry, Rats, Rats, Wistar, Seminiferous Tubules metabolism, Seminiferous Tubules ultrastructure, Sertoli Cells metabolism, Sertoli Cells ultrastructure, Sperm Count, Spermatozoa cytology, Spermatozoa drug effects, Leydig Cells drug effects, Nanoparticles administration & dosage, Seminiferous Tubules drug effects, Sertoli Cells drug effects, Spermatogenesis drug effects, Titanium administration & dosage
- Abstract
We studied immunohistochemical and morphometric characteristics of the spermatogenic epithelium in rats against the background of peroral administration of nanoparticles containing titanium dioxide. Substantial degenerative changes in the spermatogenic epithelium were revealed: thinning, disorganization of layers, and detachment of sperm cells from the basement membrane. Immunohistochemical analysis revealed reduced proliferative activity and differentiation potential of epithelial cells, which was confirmed by changes in the expression of Ki-67 and c-kit markers. Our data attest to unfavorable effects of titanium dioxide nanoparticles on the structural and functional characteristics of the reproductive system in male rats leading to spermatogenesis disturbances.
- Published
- 2018
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